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Human CD2 Antibody

Catalog #: MAB18562 Datasheet
Catalog # Availability Size / Price Qty
MAB18562-100
MAB18562-SP
Detection of Human CD2 by Western Blot.
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Human CD2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 299808
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD2
Lys25-Asp209
Accession # P06729
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human CD2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Ramos human Burkitt's lymphoma cell line (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD2 Monoclonal Antibody (Catalog # MAB18562) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD2 at approximately 50 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry Detection of CD2 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD2 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD2 Monoclonal Antibody (Catalog # MAB18562) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002), followed by PE-conjugated anti-Mouse IgG secondary antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD2

CD2 is a type I glycoprotein belonging to the Ig superfamily and is expressed on T, NK, B, and some antigen presenting cells. Human CD2 binds human CD58 on antigen-presenting cells to induce co-stimulating signals in T cells.

Entrez Gene IDs
914 (Human); 12481 (Mouse); 102120798 (Cynomolgus Monkey)
Alternate Names
CD2 antigen (p50), sheep red blood cell receptor; CD2 antigen; CD2 molecule; CD2; Erythrocyte receptor; FLJ46032; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; Rosette receptor; SRBC; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5

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