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Human CCL8/MCP-2 Antibody

Catalog #: AF-281-NA
1 Citation Datasheet
Catalog # Availability Size / Price Qty
AF-281-NA
AF-281-SP
Chemotaxis Induced by CCL8/MCP‑2 and Neutralization by Human CCL8/MCP‑2 Antibody.
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Human CCL8/MCP-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL8/MCP‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 40% cross‑reactivity with recombinant human (rh) HCC-1 is observed, less than 10% cross‑reactivity with rhEotaxin is observed, and less than 5% cross‑reactivity with rhMCP-1 is observed. Neutralizes the biological activity of rhCCL8. This antibody will not neutralize the biological activity of rhMCP-1, recombinant mouse JE, or rhMCP-3.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL8/MCP-2
Gln24-Pro99
Accession # P80075
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CCL8/MCP‑2 (Catalog # 281-CP)
Neutralization
Measured by its ability to neutralize CCL8/MCP-2-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically <20.0 µg/mL in the presence of 250 ng/mL Recombinant Human CCL8/MCP-2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization View Larger

Chemotaxis Induced by CCL8/MCP‑2 and Neutralization by Human CCL8/MCP‑2 Antibody. Human CCL8/MCP‑2 Antibody (Catalog # AF-281-NA) neutralizes CCL8/MCP-2-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically <20.0 µg/mL in the presence of 250 ng/mL Recombinant Human CCL8/MCP-2.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL8/MCP-2

MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2.

Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Entrez Gene IDs
6355 (Human); 20307 (Mouse)
Alternate Names
C-C motif chemokine 8; CCL8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; member 8 (monocyte chemotactic protein 2); monocyte chemoattractant protein 2; SCYA10; small-inducible cytokine A8

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Citation for Human CCL8/MCP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Expression and regulation of chemokines in murine and human type 1 diabetes.
    Authors: Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D
    Diabetes, 2011-12-30;61(2):436-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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