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Human CCL5/RANTES Antibody

Catalog #: MAB2781
2 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB2781-SP
MAB2781-500
MAB2781-100
Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL5/RANTES Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human CCL1, 2, 3, 4, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 8, 9/10/MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, recombinant cotton rat CCL3, 4, 5, or recombinant rat CCL20.
Source
Monoclonal Mouse IgG1 Clone # 16411
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CCL5/RANTES
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CCL5/RANTES (Catalog # 278-RN)
Neutralization
Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 0.01 µg/mL Recombinant Human CCL5/RANTES.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody. View Larger

Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody. Recombinant Human CCL5/RANTES (Catalog # 278-RN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB2781). The ND50 is typically 0.2-0.8 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL5/RANTES

RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily and is now designated CCL5. It binds and activates the chemokine receptors CCR1, CCR3, and CCR5.

Entrez Gene IDs
6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES

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Citations for Human CCL5/RANTES Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Tick-Borne Encephalitis Virus Nonstructural Protein NS5 Induces RANTES Expression Dependent on the RNA-Dependent RNA Polymerase Activity
    Authors: Z Zheng, J Yang, X Jiang, Y Liu, X Zhang, M Li, M Zhang, M Fu, K Hu, H Wang, MH Luo, P Gong, Q Hu
    J. Immunol., 2018-05-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Treatment of Inflamed Pancreas with Enkephalin Encoding HSV-1 Recombinant Vector Reduces Inflammatory Damage and Behavioral Sequelae.
    Authors: Lu Y, McNearney TA, Lin W, Wilson SP, Yeomans DC, Westlund KN
    Mol. Ther., 2007-06-12;15(10):1812-9.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P

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