Human CCL21/6Ckine Antibody Summary
rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, or rmCCL7.
Ser24-Pro134
Accession # O00585
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL21/6Ckine
6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (Secondary Lymphoid-tissue Chemokine), CCL21 and Exodus-2, shows 21 - 33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxyl-terminal domain. Human 6Ckine cDNA encodes a 134 amino acid residue, highly basic, precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. The gene for human 6Ckine has been localized at human chromosome 9p13 rather than chromosome 17 where the genes of many human CC chemokines are clustered. The 6Ckine gene location is within a region of about 100 kb from the gene for
MIP‑3 beta /ELC, another novel CC chemokine.
Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. Recombinant mouse 6Ckine is chemotactic in vitro for thymocytes and activated T cells. Recombinant human 6Ckine has been shown to be chemotactic for some human T cell lines, resting PBL, and cultured T cells expanded with PHA and IL-2. 6Ckine has also been reported to inhibit hemopoietic progenitor colony formation in a dose-dependent manner. 6Ckine acts via a class of as yet unidentified CC receptors on both T cells and B cells that are not shared by any other CC chemokines so far tested.
- Hedrick, J.A. and A. Zlotnik (1997) J. Immunol. 159:1589.
- Hromas, R. et al. (1997) J. Immunol. 159:2554.
- Nagira, M. et al. (1997) J. Biol. Chem. 272:19518.
Product Datasheets
Citations for Human CCL21/6Ckine Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Target organ expression and biomarker characterization of chemokine CCL21 in systemic sclerosis associated pulmonary arterial hypertension
Authors: Henriette Didriksen, Øyvind Molberg, Adi Mehta, Suzana Jordan, Vyacheslav Palchevskiy, Håvard Fretheim et al.
Frontiers in Immunology
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Cancer associated fibroblasts (CAFs) are activated in cutaneous basal cell carcinoma and in the peritumoural skin
Authors: SH Omland, EE Wettergren, S Mollerup, M Asplund, T Mourier, AJ Hansen, R Gniadecki
BMC Cancer, 2017-10-07;17(1):675.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Inflammation-induced secretion of CCL21 in lymphatic endothelium is a key regulator of integrin-mediated dendritic cell transmigration.
Authors: Johnson LA, Jackson DG
Int. Immunol., 2010-08-25;22(10):839-49.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Expression of podoplanin and other lymphatic markers in the human anterior eye segment.
Authors: Birke K, Lutjen-Drecoll E, Kerjaschki D, Birke MT
Invest. Ophthalmol. Vis. Sci., 2009-09-08;51(1):344-54.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Therapeutic targeting of CC ligand 21 or CC chemokine receptor 7 abrogates pulmonary fibrosis induced by the adoptive transfer of human pulmonary fibroblasts to immunodeficient mice.
Authors: Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Flaherty KR, Martinez FJ, Hogaboam CM
Am. J. Pathol., 2007-04-01;170(4):1152-64.
Species: Complex Species Category
Sample Types: In Vivo
Applications: Neutralization
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