Human CCL20/MIP-3 alpha Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 66.2 | 180 | 372 | 68.7 | 186 | 357 |
Standard Deviation | 1.84 | 3.83 | 15.1 | 4.51 | 13 | 28.8 |
CV% | 2.8 | 2.1 | 4.1 | 6.6 | 6.9 | 8.1 |
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 55.9 | 161 | 323 | 72.5 | 200 | 386 |
Standard Deviation | 1.62 | 4.28 | 9.1 | 6.05 | 17.9 | 35.3 |
CV% | 2.9 | 2.7 | 2.8 | 8.3 | 8.9 | 9.1 |
Recovery
The recovery of MIP-3 alpha spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=8) | 101 | 88-115 |
EDTA Plasma (n=5) | 104 | 97-115 |
Heparin Plasma (n=5) | 104 | 98-112 |
Serum (n=5) | 103 | 94-112 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: CCL20/MIP-3 alpha
CCL20, also known as MIP-3 alpha, is a chemokine that plays an important role in dendritic cell trafficking and recruitment and activation of T cells. It is upregulated in monocytes, T cells, endothelial cells, epithelial cells, and fibroblasts following inflammatory stimulation. CCL20 signals through CCR6 which is expressed on memory T cells, B cells and bone marrow-derived dendritic cells.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human CCL20/MIP-3 alpha Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Activation of the ?7nAChR by GTS-21 mitigates septic tubular cell injury and modulates macrophage infiltration
Authors: Yang, A;Wu, CH;Matsuo, S;Umene, R;Nakamura, Y;Inoue, T;
International immunopharmacology
Species: Human
Sample Types: Plasma
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Single cell transcriptomics identifies distinct profiles in pediatric acute respiratory distress syndrome
Authors: Flerlage, T;Crawford, JC;Allen, EK;Severns, D;Tan, S;Surman, S;Ridout, G;Novak, T;Randolph, A;West, AN;Thomas, PG;
Nature communications
Species: Human
Sample Types: Tracheal Aspirate
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Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells
Authors: F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber
bioRxiv : the preprint server for biology, 2023-02-13;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Anti-Psoriatic Effect of Rheum palmatum L. and Its Underlying Molecular Mechanisms
Authors: LTH Nguyen, SH Ahn, HM Shin, IJ Yang
International Journal of Molecular Sciences, 2022-12-15;23(24):.
Species: Human
Sample Types: Tissue Lysate
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Tumor Necrosis Factor-alpha-Induced C-C Motif Chemokine Ligand 20 Expression through TNF Receptor 1-Dependent Activation of EGFR/p38 MAPK and JNK1/2/FoxO1 or the NF-kappaB Pathway in Human Cardiac Fibroblasts
Authors: CM Yang, CC Yang, WH Hsu, LD Hsiao, HC Tseng, YF Shih
International Journal of Molecular Sciences, 2022-08-13;23(16):.
Species: Human
Sample Types: Cell Culture Supernates
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Single cell transcriptomic analysis of human amnion identifies cell-specific signatures associated with membrane rupture and parturition
Authors: Wang W, Lin Y, Zhang F et al.
Cell Biosci.
Species: Human
Sample Types: Tissue Homogenates
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3, 3'- diindolylmethane hinders IL-17A/IL-17RA interaction and mitigates imiquimod-induced psoriasiform in mice
Authors: M Srikanth, M Rasool
International immunopharmacology, 2022-04-26;109(0):108795.
Species: Human
Sample Types: Serum
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EBV+ tumors exploit tumor cell-intrinsic and -extrinsic mechanisms to produce regulatory T cell-recruiting chemokines CCL17 and CCL22
Authors: A Jorapur, LA Marshall, S Jacobson, M Xu, S Marubayash, M Zibinsky, DX Hu, O Robles, JJ Jackson, V Baloche, P Busson, D Wustrow, DG Brockstedt, O Talay, PD Kassner, G Cutler
PloS Pathogens, 2022-01-13;18(1):e1010200.
Species: Human
Sample Types: Cell Culture Supernates
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PERK signaling through C/EBPdelta contributes to ER stress-induced expression of immunomodulatory and tumor promoting chemokines by cancer cells
Authors: N Sheshadri, DK Poria, S Sharan, Y Hu, C Yan, VN Koparde, K Balamuruga, E Sterneck
Cell Death & Disease, 2021-11-01;12(11):1038.
Species: Human
Sample Types: Cell Culture Supernates
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Systemic and mucosal immune profiling in asymptomatic and symptomatic SARS-CoV-2-infected individuals reveal unlinked immune signatures
Authors: S Ravichandr, G Grubbs, J Tang, Y Lee, C Huang, H Golding, S Khurana
Science Advances, 2021-10-13;7(42):eabi6533.
Species: Human
Sample Types: Nasal Lavage Fluid
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FOXO1 promotes tumor progression by increased M2 macrophage infiltration in esophageal squamous cell carcinoma
Authors: Y Wang, Z Lyu, Y Qin, X Wang, L Sun, Y Zhang, L Gong, S Wu, S Han, Y Tang, Y Jia, DL Kwong, N Kam, XY Guan
Theranostics, 2020-09-16;10(25):11535-11548.
Species: Human
Sample Types: Cell Culture Supernates
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Mucosal-associated invariant T cells redistribute to the peritoneal cavity during spontaneous bacterial peritonitis and contribute to peritoneal inflammation
Authors: O Ibidapo-Ob, S Stengel, N Köse-Vogel, S Quickert, PA Reuken, M Busch, M Bauer, A Stallmach, T Bruns
Cell Mol Gastroenterol Hepatol, 2020-01-15;0(0):.
Species: Human
Sample Types: Ascites Fluid
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The EGFR-ERK/JNK-CCL20 Pathway in Scratched Keratinocytes May Underpin Koebnerization in Psoriasis Patients
Authors: K Furue, T Ito, Y Tanaka, A Hashimoto-, M Takemura, M Murata, M Kido-Nakah, G Tsuji, T Nakahara, M Furue
Int J Mol Sci, 2020-01-09;21(2):.
Species: Human
Sample Types: Cell Culture Supernatants
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Epigenetic downregulation of STAT6 increases HIF-1α expression via mTOR/S6K/S6, leading to enhanced hypoxic viability of glioma cells
Authors: SJ Park, H Kim, SH Kim, EH Joe, I Jou
Acta Neuropathol Commun, 2019-09-17;7(1):149.
Species: Human
Sample Types: Cell Culture Supernates
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Cyto/chemokine profile of in vitro scratched keratinocyte model: Implications of significant upregulation of CCL20, CXCL8 and IL36G in Koebner phenomenon
Authors: K Furue, T Ito, Y Tanaka, A Yumine, A Hashimoto-, M Takemura, M Murata, K Yamamura, G Tsuji, M Furue
J. Dermatol. Sci., 2019-04-13;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Staphylococcal Superantigens Stimulate Epithelial Cells through CD40 To Produce Chemokines
Authors: PM Schlievert, MP Cahill, BS Hostager, AJ Brosnahan, AJ Klingelhut, FA Gourronc, GA Bishop, DYM Leung
MBio, 2019-03-19;10(2):.
Species: Human
Sample Types: Cell Culture Supernates
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Th17-associated cytokines multiplex testing indicates the potential of macrophage inflammatory protein-3 alpha in the diagnosis of biliary atresia
Authors: P Chen, Z Zhong, H Jiang, H Chen, J Lyu, L Zhou
Cytokine, 2019-01-23;116(0):21-26.
Species: Human
Sample Types: Serum
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Effects of modified FOLFOX-6 chemotherapy on cellular immune function in patients with gastric cancer
Authors: L Wang, D Zhou, H Ren, Y Chen
Oncol Lett, 2018-03-28;15(6):8635-8640.
Species: Human
Sample Types: Cell Culture Supernates
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CCL20 secreted from IgA1-stimulated human mesangial cells recruits inflammatory Th17 cells in IgA nephropathy
Authors: G Lu, X Zhang, L Shen, Q Qiao, Y Li, J Sun, J Zhang
PLoS ONE, 2017-05-26;12(5):e0178352.
Species: Human
Sample Types: Serum
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Fusobacterium nucleatum subspecies animalis influences pro-inflammatory cytokine expression and monocyte activation in human colorectal tumors
Authors: X Ye, R Wang, R Bhattachar, D Boulbes, F Fan, L Xia, H Adoni, NJ Ajami, MC Wong, DP Smith, JF Petrosino, S Venable, W Qiao, V Baladanday, DM Maru, LM Ellis
Cancer Prev Res (Phila), 2017-05-08;0(0):.
Species: Human
Sample Types: Tissue Homogenates
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Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization
Authors: Valentina Cecchinato
J. Immunol, 2016-11-28;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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CCL20 mediates RANK/RANKL-induced epithelial-mesenchymal transition in endometrial cancer cells
Oncotarget, 2016-05-03;7(18):25328-39.
Species: Human
Sample Types: Cell Culture Supernates, Serum
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CCL20-CCR6 Cytokine Network Facilitate Treg Activity in Advanced Grades and Metastatic Variants of Hepatocellular Carcinoma.
Authors: Li W, Liu H
Scand J Immunol, 2016-01-01;83(1):33-7.
Species: Human
Sample Types: Tissue Homogenates
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Plasticity of airway epithelial cell transcriptome in response to flagellin.
Authors: Clark J, Kim K, Basom R, Gharib S
PLoS ONE, 2015-02-10;10(2):e0115486.
Species: Human
Sample Types: Cell Culture Supernates
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Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination.
Authors: Zhan Z, Xie X, Cao H, Zhou X, Zhang X, Fan H, Liu Z
Autophagy, 2013-12-04;10(2):257-68.
Species: Human
Sample Types: Cell Culture Supernates
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IL-7 licenses activation of human liver intrasinusoidal mucosal-associated invariant T cells.
Authors: Tang, Xin-Zi, Jo, Juandy, Tan, Anthony, Sandalova, Elena, Chia, Adeline, Tan, Kai Chah, Lee, Kang Hoe, Gehring, Adam J, De Libero, Gennaro, Bertoletti, Antonio
J Immunol, 2013-02-27;190(7):3142-52.
Species: Human
Sample Types: Whole Cells
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Selective impact of HIV disease progression on the innate immune system in the human female reproductive tract.
Authors: Lahey T, Ghosh M, Fahey JV, Shen Z, Mukura LR, Song Y, Cu-Uvin S, Mayer KH, Wright PF, Kappes JC, Ochsenbauer C, Wira CR
PLoS ONE, 2012-06-04;7(6):e38100.
Species: Human
Sample Types: Vaginal Lavage Fluid
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IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
Clin. Cancer Res., 2011-11-02;17(24):7529-38.
Species: Human
Sample Types: Serum
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A randomized trial to assess anti-HIV activity in female genital tract secretions and soluble mucosal immunity following application of 1% tenofovir gel.
Authors: Keller MJ, Madan RP, Torres NM
PLoS ONE, 2011-01-25;6(1):e16475.
Species: Human
Sample Types: Vaginal Lavage Fluid
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Activation of the aryl hydrocarbon receptor reveals distinct requirements for IL-22 and IL-17 production by human T helper cells.
Authors: Ramirez JM, Brembilla NC, Sorg O, Chicheportiche R, Matthes T, Dayer JM, Saurat JH, Roosnek E, Chizzolini C
Eur. J. Immunol., 2010-09-01;40(9):2450-9.
Species: Human
Sample Types: Cell Culture Supernates
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Characterization of circulating dendritic cells in melanoma: role of CCR6 in plasmacytoid dendritic cell recruitment to the tumor.
Authors: Charles J, Di Domizio J, Salameire D, Bendriss-Vermare N, Aspord C, Muhammad R, Lefebvre C, Plumas J, Leccia MT, Chaperot L
J. Invest. Dermatol., 2010-03-11;130(6):1646-56.
Species: Human
Sample Types: Cell Culture Supernates
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Opposing roles of IL-17A and IL-25 in the regulation of TSLP production in human nasal epithelial cells.
Authors: Xu G, Zhang L, Wang DY, Xu R, Liu Z, Han DM, Wang XD, Zuo KJ, Li HB
Allergy, 2009-11-25;65(5):581-9.
Species: Human
Sample Types: Cell Culture Supernates
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Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
Cancer Res., 2009-07-28;69(15):6249-55.
Species: Human
Sample Types: Cell Culture Supernates
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The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis.
Authors: Narbutt J, Lesiak A, Sysa-Jedrzeiowska A, Zakrzewski M, Bogaczewicz J, Stelmach I, Kuna P
Mediators Inflamm., 2009-07-22;2009(0):269541.
Species: Human
Sample Types: Serum
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Th17 Cytokines Stimulate CCL20 Expression in Keratinocytes In Vitro and In Vivo: Implications for Psoriasis Pathogenesis.
Authors: Harper EG, Guo C, Rizzo H, Lillis JV, Kurtz SE, Skorcheva I, Purdy D, Fitch E, Iordanov M, Blauvelt A
J. Invest. Dermatol., 2009-03-19;129(0):2175.
Species: Human
Sample Types: Cell Culture Supernates
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Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling.
Authors: Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R
J. Exp. Med., 2009-03-09;206(3):535-48.
Species: Human
Sample Types: Cell Culture Supernates
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Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes.
Authors: Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P
J. Immunol., 2009-03-01;182(0):3112-20.
Species: Human
Sample Types: Cell Culture Supernates
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HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
J. Exp. Med., 2009-01-12;206(1):169-81.
Species: Human
Sample Types: Cell Culture Supernates
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Macrophage inflammatory protein-3alpha is a novel serum marker for nasopharyngeal carcinoma detection and prediction of treatment outcomes.
Authors: Chang KP, Hao SP, Chang JH, Wu CC, Tsang NM, Lee YS, Hsu CL, Ueng SH, Liu SC, Liu YL, Wei PC, Liang Y, Chang YS, Yu JS
Clin. Cancer Res., 2008-11-01;14(21):6979-87.
Species: Human
Sample Types: Serum
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Evidence for a pathogenetic role of interleukin-18 in cutaneous lupus erythematosus.
Authors: Wang D, Drenker M, Eiz-Vesper B, Werfel T, Wittmann M
Arthritis Rheum., 2008-10-01;58(10):3205-15.
Species: Human
Sample Types: Cell Culture Supernates
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Urinary fractalkine is a marker of acute rejection.
Authors: Peng W, Chen J, Jiang Y, Wu J, Shou Z, He Q, Wang Y, Chen Y, Wang H
Kidney Int., 2008-09-17;74(11):1454-60.
Species: Human
Sample Types: Urine
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Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
Authors: Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
J. Neuroimmunol., 2008-06-09;199(1):35-45.
Species: Human
Sample Types: Cell Culture Supernates
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Expression of the Epstein-Barr virus-encoded Epstein-Barr virus nuclear antigen 1 in Hodgkin's lymphoma cells mediates Up-regulation of CCL20 and the migration of regulatory T cells.
Authors: Baumforth KR, Birgersdotter A, Reynolds GM, Wei W, Kapatai G, Flavell JR, Kalk E, Piper K, Lee S, Machado L, Hadley K, Sundblad A, Sjoberg J, Bjorkholm M, Porwit AA, Yap LF, Teo S, Grundy RG, Young LS, Ernberg I, Woodman CB, Murray PG
Am. J. Pathol., 2008-05-23;173(1):195-204.
Species: Human
Sample Types: Cell Culture Supernates
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IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood.
Authors: Zrioual S, Toh ML, Tournadre A, Zhou Y, Cazalis MA, Pachot A, Miossec V, Miossec P
J. Immunol., 2008-01-01;180(1):655-63.
Species: Human
Sample Types: Cell Culture Supernates
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Airway dendritic cell phenotypes in inflammatory diseases of the human lung.
Authors: Lommatzsch M, Bratke K, Bier A, Julius P, Kuepper M, Luttmann W, Virchow JC
Eur. Respir. J., 2007-07-11;30(5):878-86.
Species: Human
Sample Types: BALF
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Specificity of immunomodulator secretion in urinary samples in response to infection by alpha-hemolysin and CNF1 bearing uropathogenic Escherichia coli.
Authors: Real JM, Munro P, Buisson-Touati C, Lemichez E, Boquet P, Landraud L
Cytokine, 2007-03-26;37(1):22-5.
Species: Human
Sample Types: Urine
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Accumulation of dendritic cells and increased CCL20 levels in the airways of patients with chronic obstructive pulmonary disease.
Authors: Demedts IK, Bracke KR, Van Pottelberge G, Testelmans D, Verleden GM, Vermassen FE, Joos GF, Brusselle GG
Am. J. Respir. Crit. Care Med., 2007-03-01;175(10):998-1005.
Species: Human
Sample Types: Sputum
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An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
J. Exp. Med., 2006-11-20;203(12):2763-77.
Species: Human
Sample Types: Cell Culture Supernates
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Trichomonas vaginalis lipophosphoglycan triggers a selective upregulation of cytokines by human female reproductive tract epithelial cells.
Authors: Fichorova RN, Trifonova RT, Gilbert RO, Costello CE, Hayes GR, Lucas JJ, Singh BN
Infect. Immun., 2006-10-01;74(10):5773-9.
Species: Human
Sample Types: Cell Culture Supernates
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Hypoxia modifies the transcriptome of primary human monocytes: modulation of novel immune-related genes and identification of CC-chemokine ligand 20 as a new hypoxia-inducible gene.
Authors: Bosco MC, Puppo M, Santangelo C, Anfosso L, Pfeffer U, Fardin P, Battaglia F, Varesio L
J. Immunol., 2006-08-01;177(3):1941-55.
Species: Human
Sample Types: Cell Culture Supernates
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Expression of CCL20 and granulocyte-macrophage colony-stimulating factor, but not Flt3-L, from modified vaccinia virus ankara enhances antiviral cellular and humoral immune responses.
Authors: Chavan R, Marfatia KA, An IC, Garber DA, Feinberg MB
J. Virol., 2006-08-01;80(15):7676-87.
Species: Human
Sample Types: Cell Culture Supernates
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Glycerol monolaurate inhibits the effects of Gram-positive select agents on eukaryotic cells.
Authors: Peterson ML, Schlievert PM
Biochemistry, 2006-02-21;45(7):2387-97.
Species: Human
Sample Types: Cell Culture Supernates
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Role for cysteinyl leukotrienes in allergen-induced change in circulating dendritic cell number in asthma.
Authors: Parameswaran K, Liang H, Fanat A, Watson R, Snider DP, O'Byrne PM
J. Allergy Clin. Immunol., 2004-07-01;114(1):73-9.
Species: Human
Sample Types: Sputum
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