Human CCL14/HCC-1/HCC-3 Antibody

Catalog #: MAB3241
2 Citations 3 Reviews Datasheet

Catalog #	Availability	Size / Price	Qty
MAB3241
MAB3241-SP

Chemotaxis Induced by CCL14a/HCC‑1 and Neutral-ization by Human CCL14/ HCC‑1/HCC‑3 Antibody.

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All CCL14/HCC-1/HCC-3 Antibodies

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Citations (2)

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Reviews (3)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL14/HCC-1/HCC-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL14/HCC‑1/HCC‑3 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL2, 3, 4, 6, 7, 9, 11, 12, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20 is observed.

Source

Monoclonal Rat IgG_2A Clone # 256409

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CCL14/HCC‑1/HCC‑3
Thr20-Asn109
Accession # NP_116738

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CCL14a/HCC‑1 aa 20-93 (Catalog # 324-HC)

Human CCL14a/HCC-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human CCL14a/HCC‑1 Biotinylated Antibody (Catalog # BAF324)

Standard: Recombinant Human CCL14a/HCC-1 Protein (Catalog # 324-HC)

Neutralization

Measured by its ability to neutralize CCL14a/HCC‑1 -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND₅₀) is typically 0.5-2.5 µg/mL in the presence of 10 ng/mL Recombinant Human CCL14a/HCC‑1 aa 28‑93.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL14a/HCC‑1 and Neutral-ization by Human CCL14/ HCC‑1/HCC‑3 Antibody. Recombinant Human CCL14a/ HCC-1 (Catalog # 1578-HC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL14a/HCC-1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCL14/HCC-1/ HCC-3 Monoclonal Antibody (Catalog # MAB3241). The ND₅₀ is typically 0.5-2.5 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL14/HCC-1/HCC-3

CCL14 is a chemokine that promotes chemotaxis of T cells, monocytes, and eosinophils. It occurs in two isoforms resulting from differential mRNA splicing. Following cleavage of a 19 amino acid signal peptide, mature CCL14a (aa 20‑93) is a 74 amino acid peptide that is also known as HCC-1 (Hemofiltrate CC Chemokine-1). It is a weak CCR1 agonist, however, an 8 amino acid N-terminal truncation (aa 28‑93) allows potent signaling through CCR1 and CCR5. CCL14b, also known as HCC-3, is a 90 amino acid peptide (aa 20‑109) resulting from the insertion of 16 amino acids between residues 7 and 8 of CCL14a.

Entrez Gene IDs

6358 (Human)

Alternate Names

C-C motif chemokine 14; CCL14; chemokine (C-C motif) ligand 14; Chemokine CC-1/CC-3; chemokine CC-3; CKB1; FLJ16015; HCC-1; HCC-1(1-74); HCC-1/HCC-3; HCC-3; hemofiltrate CC chemokine 1; MCIF; member 14; NCC2CC-1; NCC-2CKb1; new CC chemokine 2; SCYA14CC-3; Small-inducible cytokine A14

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human CCL14/HCC-1/HCC-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Crosstalk between macrophage-derived PGE2 and tumor UHRF1 drives hepatocellular carcinoma progression
Authors: J Zhang, H Zhang, X Ding, J Hu, Y Li, J Zhang, H Wang, S Qi, A Xie, J Shi, M Xiang, Y Bin, G Wang, L Wang, Z Wang
Theranostics, 2022-05-01;12(8):3776-3793.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Neutralization, Western Blot
Chemokines CCL2, 3, 14 stimulate macrophage bone marrow homing, proliferation, and polarization in multiple myeloma
Authors: Yi Li, Yuhuan Zheng, Tianshu Li, Qiang Wang, Jianfei Qian, Yong Lu et al.
Oncotarget