Human CCL14/HCC-1/HCC-3 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB324R Datasheet

Catalog #	Availability	Size / Price	Qty
FAB324R-100UG

All CCL14a/HCC-1 Antibodies

Product Details

FAQs

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL14/HCC-1/HCC-3 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL14/HCC‑1/HCC‑3 in direct ELISAs and Western blots. Detects both CCL14a and CCL14b. In Western blots, no cross‑reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, recombi

Source

Monoclonal Rat IgG2a Clone # 256413

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human CCL14/HCCâ€‘1/HCCâ€‘3
Thr20-Asn109
Accession # NP_116738

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CCL14a/HCC-1

CCL14 is a chemokine that promotes chemotaxis of T cells, monocytes, and eosinophils. It occurs in two isoforms resulting from differential mRNA splicing. Following cleavage of a 19 amino acid signal peptide, mature CCL14a (aa 20‑93) is a 74 amino acid peptide that is also known as HCC-1 (Hemofiltrate CC Chemokine-1). It is a weak CCR1 agonist, however, an 8 amino acid N-terminal truncation (aa 28‑93) allows potent signaling through CCR1 and CCR5. CCL14b, also called HCC-3, is a 90 amino acid peptide (aa 20‑109) resulting from the insertion of 16 amino acids between residues 7 and 8 of CCL14a.

Entrez Gene IDs

6358 (Human)

Alternate Names

C-C motif chemokine 14;CC-1;CC-3;CKB1;HCC-1;HCC-1(1-74);HCC-1/HCC-3;HCC-3;MCIF;NCC2;NCC-2;SCYA14;SCYL2;SY14; CCL14; CCL14a; HCC1; HCC-1