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Human CCL11/Eotaxin Antibody

Catalog #: MAB3201
1 Citation Datasheet
Catalog # Availability Size / Price Qty
MAB3201
MAB3201-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL11/Eotaxin Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL11/Eotaxin in Western blots. Does not cross-react with recombinant cotton rat CCL3, 4, 5, recombinant human CCL1, 3, 4, 5, 8, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 8, 9/10/MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, or recombinant rat CCL20.
Source
Monoclonal Mouse IgG1 Clone # 43915
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CCL11/Eotaxin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CCL11/Eotaxin (Catalog # 320-EO)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
WS‑1 human fetal skin fibroblast cell line treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA) and Recombinant Human IL‑4 (Catalog # 204-IL) fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL11/Eotaxin

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2 and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1-3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4).

References
  1. Kitamura, M. et al. (1996) J. Biol. Chem 271:7725.
  2. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
  3. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
  4. Choe, H. et al. (1996) Cell 85:1135.
 
Entrez Gene IDs
6356 (Human); 20292 (Mouse)
Alternate Names
C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

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Citation for Human CCL11/Eotaxin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Multi-omic longitudinal study reveals immune correlates of clinical course among hospitalized COVID-19 patients
    Authors: Joann Diray-Arce, Slim Fourati, Naresh Doni Jayavelu, Ravi Patel, Cole Maguire, Ana C. Chang et al.
    Cell Reports Medicine

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