Human Cathepsin V Biotinylated Antibody

Catalog #: BAF1080 Datasheet
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BAF1080
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Human Cathepsin V Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cathepsin V in Western blots. In this format, approximately 15% cross-reactivity with recombinant human (rh) Cathepsin C, rhCathepsin A, rhCathepsin D, rhCathepsin O is observed and less than 5% cross-reactivity with rhCathepsin S and rhCathepsin X/Z/P is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin V
Val18-Val334
Accession # O60911
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Cathepsin V (Catalog # 1080-CY)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin V

Cathepsin V (also known as Cathepsin L2 and U) is a cysteine protease of the papain family (1). It is expressed as a proenzyme in lysosomes, which can be autocatalytically converted to the mature form at pH 4.0 (2). Cathepsin V shows high amino acid identity with Cathepsin L (77% for the proenzymes and 80% for the mature enzymes) (3). However, Cathepsin V is specifically expressed in the thymus, testis, and corneal epithelium whereas Cathepsin L is expressed throughout the body.

References
  1. Turk, V. et al. (2001) EMBO J. 20:4629.
  2. Bromme, D. et al. (1999) Biochemistry 38:2377.
  3. Somoza, J.R. et al. (2000) Biochemistry 39:12543.
Entrez Gene IDs
1515 (Human)
Alternate Names
Cathepsin L2; Cathepsin U; Cathepsin V; CATL2; CTSL2; CTSUcathepsin L2, preproprotein; CTSVMGC125957; EC 3.4.22; EC 3.4.22.43

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