Human Catalase Antibody Summary
Met1-Leu527
Accession # P04040
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Catalase by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Catalase Monoclonal Antibody (Catalog # MAB3398) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Catalase at approximately 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Catalase by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for Catalase at approximately 61 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Catalase Monoclonal Antibody (Catalog # MAB3398). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Catalase in HL‑60 Human Cell Line. Catalase was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human Catalase Monoclonal Antibody (Catalog # MAB3398) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to peroxisomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Western Blot Shows Human Catalase Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Catalase knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Catalase Monoclonal Antibody (Catalog # MAB3398) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Catalase at approximately 64 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Catalase
Cells have evolved complex mechanisms to maintain redox balance and defend against oxidative stress. Catalase is a tetrameric enzyme comprised of four 60 kDa subunits. Catalase is typically localized in the peroxisome where it functions as an antioxidant, protecting cells from damage due to oxidative stress. Catalase converts reactive oxygen species, such as H2O2, into water and O2. Human Catalase shares 89% homology to mouse and rat Catalase. The cells redox environment can serve as an important signaling switch or trigger to initiate a number of cellular processes, including gene expression, differentiation, proliferation and apoptosis.
Product Datasheets
Citations for Human Catalase Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Nox4 mediates skeletal muscle metabolic responses to exercise
Authors: KS Specht, S Kant, AK Addington, RP McMillan, MW Hulver, H Learnard, M Campbell, SR Donnelly, AD Caliz, Y Pei, MM Reif, JM Bond, A DeMarco, B Craige, JF Keaney, SM Craige
Molecular Metabolism, 2021-01-02;45(0):101160.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Rejuvenation of mesenchymal stem cells by extracellular vesicles inhibits the elevation of reactive oxygen species
Authors: Vuong Cat Khanh, Toshiharu Yamashita, Kinuko Ohneda, Chiho Tokunaga, Hideyuki Kato, Motoo Osaka et al.
Scientific Reports
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BNIP3L/NIX regulates both mitophagy and pexophagy
Authors: Wilhelm LP, Zapata-MuNoz J, Villarejo-Zori B et al.
The EMBO journal
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