Human Caspase-8 Antibody

Catalog #: MAB704
5 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
MAB704
MAB704-SP

Detection of Human Caspase‑8 by Western Blot.

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Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Caspase-8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Caspase-8 precursor in Western blots and a 42 kDa doublet generated during apoptosis.

Source

Monoclonal Mouse IgG_2A Clone # 84131

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human Caspase-8
aa 234-391

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Caspase‑8 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with Human FAS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF126) for indicated times. PVDF membrane was probed with 1 µg/mL of Human Caspase-8 Monoclonal Antibody (Catalog # MAB704), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Caspase-8 at approximately 55 and 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspase-8

Caspase-8 (Cysteine-aspartic acid protease 8/Casp8; also MCH5 and FLICA) is a 28 kDa member of the peptidase C14A family of enzymes. It is known as the initiating caspase for the apoptotic cascade. Caspase-8 acts on procaspases-3, 4, 6, 7, 9 and 10, in addition to c-FLIPL and procaspase-8 itself. Human procaspase-8 alpha 4 is a 58 kDa (predicted), 496 amino acid (aa) protein and contains two N-terminal death domains plus a catalytic site that utilizes His334Gly335 plus Cys377. Following death domain-containing receptor activation, proteolysis generates a 28 kDa heterodimer. This includes a p18 subunit (aa 234-391; or 217-374 of the 8a/standard form) noncovalently linked to a p10 subunit (aa 402-496; or 385-479 of the 8a/standard form). Association with another p18/p10 heterodimer generates active caspase-8. There are two common procaspase-8 isoform variants. One 53 kDa isoform (8b) shows a deletion of aa 103-134, while a second 55 kDa (8a) isoform shows a deletion of aa 103-134 with a 15 aa insertion after Lys183. The p18 and p10 subunits are 68% and 82% aa identical, human to mouse, respectively.

Entrez Gene IDs

841 (Human); 12370 (Mouse)

Alternate Names

AIS; androgen receptor; CASP8; Caspase8; Caspase-8; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; Mch5; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SMAX1SBMA; spinal and bulbar muscular atrophy

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human Caspase-8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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PD-L1-mediated gasdermin C expression switches apoptosis to pyroptosis in cancer cells and facilitates tumour necrosis
Authors: J Hou, R Zhao, W Xia, CW Chang, Y You, JM Hsu, L Nie, Y Chen, YC Wang, C Liu, WJ Wang, Y Wu, B Ke, JL Hsu, K Huang, Z Ye, Y Yang, X Xia, Y Li, CW Li, B Shao, JA Tainer, MC Hung
Nat. Cell Biol., 2020-09-14;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Silencing of heat shock protein 27 increases the radiosensitivity of non?small cell lung carcinoma cells
Authors: L Xu, X Lin, Y Zheng, H Zhou
Mol Med Rep, 2019-05-22;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Regulation of the extrinsic apoptotic pathway by the extracellular matrix glycoprotein EMILIN2.
Authors: Mongiat M, Ligresti G, Marastoni S, Lorenzon E, Doliana R, Colombatti A
Mol. Cell. Biol., 2007-08-13;27(20):7176-87.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Enhanced susceptibility to tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis in oral squamous cell carcinoma cells treated with phosphatidylinositol 3-kinase inhibitors.
Authors: Uchida M, Iwase M, Takaoka S, Yoshiba S, Kondo G, Watanabe H, Ohashi M, Nagumo M, Shintani S
Int. J. Oncol., 2007-05-01;30(5):1163-71.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Changes in lamina structure are followed by spatial reorganization of heterochromatic regions in caspase-8-activated human mesenchymal stem cells.
Authors: Raz V, Carlotti F, Vermolen BJ, van der Poel E, Sloos WC, Knaan-Shanzer S, de Vries AA, Hoeben RC, Young IT, Tanke HJ, Garini Y, Dirks RW
J. Cell. Sci., 2006-09-26;119(0):4247-56.
Species: Human
Sample Types: Whole Cells
Applications: ICC