Human Caspase-3 Antibody Summary
aa 29-175
Accession # P42574
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Precursor Caspase‑3 and p18 Subunit by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 µM staurosporine (STS) for 3 hours. PVDF membrane was probed with 1 µg/mL of Human Caspase-3 Monoclonal Antibody (Catalog # MAB707), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for precursor Caspase-3 and the p18 subunit at approximately 36 and 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
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Western Blot Shows Human Caspase‑3 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Caspase-3 knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Caspase-3 Monoclonal Antibody (Catalog # MAB707) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Caspase-3 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-3
Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed, and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.
Product Datasheets
Citations for Human Caspase-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Interferon-? Overexpression in Adipose Tissue-Derived Stem Cells Induces HepG2 and Macrophage Cell Death in Liver Tumor Organoids via Induction of TNF-Related Apoptosis-Inducing Ligand Expression
Authors: Yoon, Y;Kim, CW;Kim, MY;Baik, SK;Jung, PY;Eom, YW;
International journal of molecular sciences
Species: Human
Sample Types: Organoids
Applications: Western Blot -
Alpha1-antitrypsin protects lung cancer cells from staurosporine-induced apoptosis: the role of bacterial lipopolysaccharide
Authors: N Schwarz, S Tumpara, S Wrenger, E Ercetin, J Hamacher, T Welte, S Janciauski
Sci Rep, 2020-06-12;10(1):9563.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Preeclampsia is associated with alterations in the p53-pathway in villous trophoblast.
Authors: Sharp A, Heazell A, Baczyk D, Dunk C, Lacey H, Jones C, Perkins J, Kingdom J, Baker P, Crocker I
PLoS ONE, 2014-01-30;9(1):e87621.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
PMA synergistically enhances apicularen A-induced cytotoxicity by disrupting microtubule networks in HeLa cells.
Authors: Seo K, Kim J, Park J, Song K, Yun E, Park J, Kweon G, Yoon W, Lim K, Hwang B
BMC Cancer, 2014-01-22;14(0):36.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Interactions between important regulatory proteins and human alphaB crystallin.
Authors: Ghosh JG, Shenoy AK, Clark JI
Biochemistry, 2007-05-08;46(21):6308-17.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA-Based Protein Pin Array -
Developmental differences in the responses of IL-6 and IL-13 transgenic mice exposed to hyperoxia.
Authors: Choo-Wing R, Nedrelow JH, Homer RJ, Elias JA, Bhandari V
Am. J. Physiol. Lung Cell Mol. Physiol., 2007-03-30;293(1):L142-50.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Proteolytically processed soluble tumor endothelial marker (TEM) 5 mediates endothelial cell survival during angiogenesis by linking integrin alpha(v)beta3 to glycosaminoglycans.
Authors: Vallon M, Essler M
J. Biol. Chem., 2006-09-17;281(45):34179-88.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Inhibition of lymphotoxin-beta receptor-mediated cell death by survivin-DeltaEx3.
Authors: You RI, Chou YC
Cancer Res., 2006-03-15;66(6):3051-61.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
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