Human Caspase-3 Antibody

Catalog #: MAB7071 1 Review Datasheet

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB7071
MAB7071-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Caspase-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects the active form and p17 subunit of Caspase-3 in direct ELISAs, with less than 5% cross-reactivity with the precursor form observed.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1112I

Purification

Protein A or G purified from cell culture supernatant

Immunogen

KLH-conjugated human Caspase-3 synthetic peptide
CRGTELDCGIETD
Accession # P42574

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Dual RNAscope ISH-IHC

5-25 µg/mL

See below

Immunocytochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Caspase‑3 in Jurkat Human Cell Line. Caspase-3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with Staurosporine using Rabbit Anti-Human Caspase-3 Monoclonal Antibody (Catalog # MAB7071) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Dual RNAscope ISH-IHC Compatible

Caspase-3 antibody in Human Colon Using Dual RNAscope<sup>®</sup>ISH and IHC.

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Caspase-3 in Human Colon Using Dual RNAscope^®ISH and IHC. Caspase-3 mRNA was detected (top image) in formalin-fixed paraffin-embedded tissue sections of human colon probed with ACD RNAScope^®Probe (Catalog # 436561) and stained using ACD RNAscope^®2.5 HD Duplex Detection Reagents (red, Catalog # 322500). Adjacent tissue section (bottom image) was processed for immunohistochemistry using R&D Systems Rabbit Anti-Human Caspase-3 Monoclonal Antibody (Catalog# MAB7071) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001) and DAB chromogen (brown). Tissues were counterstained with hematoxylin (blue).

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspase-3

Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.

Entrez Gene IDs

836 (Human); 12367 (Mouse)

Alternate Names

Apopain; apoptosis-related cysteine protease; CASP3; CASP-3; caspase 3, apoptosis-related cysteine peptidase; Caspase3; Caspase-3; CPP32; CPP-32; CPP32B; CPP32SREBP cleavage activity 1; Cysteine protease CPP32; EC 3.4.22; EC 3.4.22.56; LICE-1; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; YAMA