Home / Carbonic Anhydrase IX/CA9 / Human Carbonic Anhydrase IX/CA9 Fluorescein-conjugated Antibody

Human Carbonic Anhydrase IX/CA9 Fluorescein-conjugated Antibody

Catalog #: FAB2188F
3 Citations 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
FAB2188F
Detection of Carbonic Anhydrase IX/CA9 in U‑87 MG Human Cell Line by Flow Cytometry.
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Citations (3)
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Reviews (2)
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Human Carbonic Anhydrase IX/CA9 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carbonic Anhydrase IX (CA9) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) CA9 or with rhCA1, 2, 3, 4, 5A, 6, 7, 8, 10, 12, 13, or 14.
Source
Monoclonal Mouse IgG2A Clone # 303123
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX
Pro59-Asp414
Accession # Q16790
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Carbonic Anhydrase IX/CA9 antibody in U-87 MG Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Carbonic Anhydrase IX/CA9 in U‑87 MG Human Cell Line by Flow Cytometry. U-87 MG human glioblastoma/ astrocytoma cell line was stained with Mouse Anti-Human Carbonic Anhydrase IX/CA9 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2188F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Carbonic Anhydrase IX/CA9

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1-3). Topics in the CA meeting (6th International Conference on the CAs, June 20-25, 2003; Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). rhCA9 corresponds to the extracellular portion of human CA9.

References
  1. Pastorek, J. et al. (1994) Oncogene 9:2877.
  2. Opavsky, R. et al. (1996) Genomics 33:480.
  3. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  4. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
  5. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
Entrez Gene IDs
768 (Human); 230099 (Mouse)
Alternate Names
CA9; CAIX; CA-IX; Carbonate dehydratase IX; carbonic anhydrase 9; Carbonic Anhydrase IX; carbonic dehydratase; EC 4.2.1.1; G250; Membrane antigen MN; MN; P54/58N; PMW1; RCC; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250

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Citations for Human Carbonic Anhydrase IX/CA9 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Bortezomib improves adoptive carbonic anhydrase�IX‑specific chimeric antigen receptor‑modified NK92 cell therapy in mouse models of human renal cell carcinoma
    Authors: Qing Zhang, Jinjing Xu, Jiage Ding, Hongyan Liu, Huizhong Li, Hailong Li et al.
    Oncology Reports
  2. Carbonic anhydrase IX promotes tumor growth and necrosis in vivo and inhibition enhances anti-VEGF therapy.
    Authors: McIntyre A, Patiar S, Wigfield S
    Clin. Cancer Res., 2012-04-12;18(11):3100-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. In Vivo Imaging and Quantification of Carbonic Anhydrase IX Expression as an Endogenous Biomarker of Tumor Hypoxia
    Authors: Bagna Bao, Kevin Groves, Jun Zhang, Emma Handy, Paul Kennedy, Garry Cuneo et al.
    PLoS ONE

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Human Carbonic Anhydrase IX/CA9 Fluorescein-conjugated Antibody
By Anonymous on 08/24/2016
Application: Flow Sample Tested: Tumour tissue Species: Human
Human Carbonic Anhydrase IX/CA9 Fluorescein-conjugated Antibody
By James Platt on 07/12/2016
Application: Flow Sample Tested: 786-O human renal cell adenocarcinoma cell line Species: Human