Home / Carbonic Anhydrase IX/CA9 / Human Carbonic Anhydrase IX/CA9 Antibody

Human Carbonic Anhydrase IX/CA9 Antibody

Catalog #: MAB21881
1 Citation 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
MAB21881
MAB21881-SP
Carbonic Anhydrase IX/CA9 in Human Colon.
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Product Details
Citations (1)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbonic Anhydrase IX/CA9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carbonic Anhydrase IX/CA9 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 303104
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX/CA9
Pro59-Asp414
Accession # Q16790
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Carbonic Anhydrase IX/CA9 antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

Carbonic Anhydrase IX/CA9 in Human Colon. Carbonic Anhydrase IX/CA9 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human Carbonic Anhydrase IX/CA9 Monoclonal Antibody (Catalog # MAB21881) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase IX/CA9

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1‑3). CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)‑associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). The immunogen corresponds to the extracellular portion of human CA9.

References
  1. Pastorek, J. et al. (1994) Oncogene 9: 2877.
  2. Opavsky, R. et al. (1996) Genomics 33: 480.
  3. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  4. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
  5. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
Entrez Gene IDs
768 (Human); 230099 (Mouse)
Alternate Names
CA9; CAIX; CA-IX; Carbonate dehydratase IX; carbonic anhydrase 9; Carbonic Anhydrase IX; carbonic dehydratase; EC 4.2.1.1; G250; Membrane antigen MN; MN; P54/58N; PMW1; RCC; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250

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Citation for Human Carbonic Anhydrase IX/CA9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Hypoxia-induced Jagged2 promotes breast cancer metastasis and self-renewal of cancer stem-like cells.
    Authors: Xing F, Okuda H, Watabe M, Kobayashi A, Pai SK, Liu W, Pandey PR, Fukuda K, Hirota S, Sugai T, Wakabayshi G, Koeda K, Kashiwaba M, Suzuki K, Chiba T, Endo M, Mo YY, Watabe K
    Oncogene, 2011-04-18;30(39):4075-86.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Reviews for Human Carbonic Anhydrase IX/CA9 Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human Carbonic Anhydrase IX/CA9 Antibody
By Anonymous on 11/26/2021
Application: IHC Sample Tested: Esophageal adenocarcinoma Species: Human
Immunohistochemistry Human Carbonic Anhydrase IX/CA9 Antibody MAB21881
Human Carbonic Anhydrase IX/CA9 Antibody
By Anonymous on 07/31/2018
Application: IHC Sample Tested: CAOV4,U87MG Species: Human