Human Carbonic Anhydrase IX/CA9 Antibody
Human Carbonic Anhydrase IX/CA9 Antibody Summary
Pro59-Asp414
Accession # Q16790
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Carbonic Anhydrase IX/CA9 in Human Stomach. Carbonic Anhydrase IX/CA9 was detected in immersion fixed paraffin-embedded sections of human stomach using Rabbit Anti-Human Carbonic Anhydrase IX/CA9 Monoclonal Antibody (Catalog # MAB11526) at 3 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of glandular cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase IX/CA9
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1‑3). CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)‑associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). The immunogen corresponds to the extracellular portion of human CA9.
- Pastorek, J. et al. (1994) Oncogene 9: 2877.
- Opavsky, R. et al. (1996) Genomics 33: 480.
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
- Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Carbonic Anhydrase IX/CA9 Antibody
There are currently no reviews for this product. Be the first to review Human Carbonic Anhydrase IX/CA9 Antibody and earn rewards!
Have you used Human Carbonic Anhydrase IX/CA9 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
