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Human Calreticulin-2 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF2927T Datasheet
Catalog # Availability Size / Price Qty
AF2927T-100UG
Product Details
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Human Calreticulin-2 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Calreticulin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Calreticulin-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Calreticulin-2
Thr20-Leu384
Accession # Q96L12
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Flow Cytometry
Optimal dilution of this antibody should be experimentally determined.
 
CyTOF-ready
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Calreticulin-2

Calreticulin-2 is a testis-specific, soluble, lectin-like molecule that participates in the protein folding and quality control cycle in the endoplasmic reticulum (ER) lumen. Mature human Calreticulin-2 has a 178 amino acid N-terminal globular domain, a 97 aa central P-domain, and a 90 aa C-terminal domain. The globular domain shows lectin activity, the P-domain binds one Ca++ ion with high affinity, and the C-terminus binds Ca++ and contains an RNEL ER retention motif. The amino acid sequence of Calreticulin-2 is 84%, 83% and 87% identical to that of mouse, bovine and canine Calreticulin-2, respectively.

Entrez Gene IDs
125972 (Human); 73316 (Mouse); 364529 (Rat)
Alternate Names
CALR3; calreticulin 2; calreticulin 3; Calreticulin2; Calreticulin-2; calreticulin-3; cancer/testis antigen 93; Crt2; CRT2calreticulin-2; CT93; FLJ25355; MGC26577

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