Human CA125/MUC16 Quantikine ELISA Kit

Catalog #: DCA125
8 Citations 5 Reviews Datasheet

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DCA125

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QC56: Quantikine Immunoassay Control Set 765 for Human CA125/MUC16

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All CA125/MUC16 ELISAs

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Citations (8)

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Reviews (5)

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human CA125/MUC16 Quantikine ELISA Kit Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (10 uL), Urine (100 uL), Human Milk (10 uL)

Sensitivity

0.099 U/mL

Assay Range

0.5 - 32 U/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)

Specificity

Natural and recombinant human CA125

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human CA125/MUC16 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CA125 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains CHO cell-expressed recombinant human CA125 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CA125 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CA125.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	4.26	10	19	4.22	9.74	18.7
Standard Deviation	0.066	0.101	0.266	0.313	0.527	0.666
CV%	1.5	1	1.4	7.4	5.4	3.6

Recovery

The recovery of CA125 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	99	95-102
EDTA Plasma (n=4)	97	93-101
Heparin Plasma (n=4)	97	92-100
Serum (n=4)	97	90-101

Linearity

To assess the linearity of the assay, samples containing high concentrations of CA125 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human CA125/MUC16 ELISA Standard Curve

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CA125/MUC16

The CA125 antigen, also known as MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. Expression of isoforms, proteolytic cleavage, and heavy N- and O- linked glycosylation produce forms of human CA125 that can vary from 200-5000 kDa in size. It inhibits NK cell activity, which is thought to contribute to immune evasion in peritoneal cancer and pregnancy.

Long Name:

Ovarian Cancer Antigen CA125

Entrez Gene IDs:

94025 (Human)

Alternate Names:

CA125 ovarian cancer antigen; CA125; CA-125; CA125MUC-16; FLJ14303; MUC16; mucin 16, cell surface associated; mucin-16; Ovarian cancer-related tumor marker CA125; Ovarian carcinoma antigen CA125

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

Prepare all reagents, standard dilutions, and samples as directed in the product insert.
Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

100 µL Assay Diluent

Add 100 µL of Assay Diluent to each well.

100 µL Standard, Control, or Sample

Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

200 µL Conjugate

Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
Aspirate and wash 4 times.

200 µL Substrate Solution

Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

50 µL Stop Solution

Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human CA125/MUC16 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Design and Fabrication of a 3D-Printed Microfluidic Immunoarray for Ultrasensitive Multiplexed Protein Detection
Authors: Hiniduma, K;Bhalerao, KS;De Silva, PIT;Chen, T;Rusling, JF;
Micromachines
Species: Human
Sample Types: Protein, Serum
Diagnostic value of circN4BP2L2 in type I and type II epithelial ovarian cancer
Authors: L Ning, J Lang, B Long, L Wu
BMC Cancer, 2022-11-24;22(1):1210.
Species: Human
Sample Types: Plasma
A novel circulating miRNA panel for non-invasive ovarian cancer diagnosis and prognosis
Authors: AW Gahlawat, T Witte, L Haarhuis, S Schott
British Journal of Cancer, 2022-08-05;0(0):.
Species: Human
Sample Types: Plasma
Plasma circN4BP2L2 is a promising novel diagnostic biomarker for epithelial ovarian cancer
Authors: L Ning, J Lang, L Wu
BMC Cancer, 2022-01-03;22(1):6.
Species: Human
Sample Types: Serum
Tumor characterization by ultrasound-release of multiple protein and microRNA biomarkers, preclinical and clinical evidence
Authors: AL D'Souza, JR Chevillet, P Ghanouni, X Yan, M Tewari, SS Gambhir
PLoS ONE, 2018-03-16;13(3):e0194268.
Species: Human
Sample Types: Plasma
Novel three dimensional human endocervix cultures respond to 28-day hormone treatment.
Authors: Arslan S, Yu Y, Burdette J, Pavone M, Hope T, Woodruff T, Kim J
Endocrinology, 2015-01-30;156(4):1602-9.
Species: Human
Sample Types: Cell Culture Supernates
Kallikrein family proteases KLK6 and KLK7 are potential early detection and diagnostic biomarkers for serous and papillary serous ovarian cancer subtypes.
Authors: Tamir, Ayala, Jag, Ushma, Sarojini, Sreeja, Schindewolf, Craig, Tanaka, Takemi, Gharbaran, Rajendra, Patel, Hiren, Sood, Anil, Hu, Wei, Patwa, Ruzeen, Blake, Patrick, Chirina, Polina, Oh Jeong, Jin, Lim, Heejin, Goy, Andre, Pecora, Andrew, Suh, K Stephe
J Ovarian Res, 2014-12-05;7(1):109.
Species: Human
Sample Types: Serum
False biomarker discovery due to reactivity of a commercial ELISA for CUZD1 with cancer antigen CA125.
Authors: Prassas, Ioannis, Brinc, Davor, Farkona, Sofia, Leung, Felix, Dimitromanolakis, Apostolo, Chrystoja, Caitlin, Brand, Randall, Kulasingam, Vathany, Blasutig, Ivan M, Diamandis, Elefther
Clin Chem, 2013-10-04;60(2):381-8.
Species: Human
Sample Types: Serum
Applications: Western Blot