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Human C-Reactive Protein/CRP Antibody

Catalog #: MAB17074 Datasheet
Catalog # Availability Size / Price Qty
MAB17074-100
MAB17074-SP
Detection of Human C‑Reactive Protein/CRP by Western Blot.
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Human C-Reactive Protein/CRP Antibody Summary

Species Reactivity
Human
Specificity
Detects human C-Reactive Protein/CRP in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 987318
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human C‑Reactive Protein/CRP
Phe17-Pro224
Accession # P02741
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human C-Reactive Protein/CRP antibody by Western Blot. View Larger

Detection of Human C‑Reactive Protein/CRP by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human C-Reactive Protein/CRP Monoclonal Antibody (Catalog # MAB17074) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for C-Reactive Protein/CRP at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C-Reactive Protein/CRP

CRP is a secreted protein that belongs to the Pentraxin family. It circulates as a non-covalent homopentamer. The CRP concentration in human plasma is highly increased during acute phase response to tissue injury or infection.

Entrez Gene IDs
1401 (Human); 12944 (Mouse); 25419 (Rat)
Alternate Names
C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895

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