Human BLNK Alexa Fluor® 750-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: BLNK

BLNK (B cell linker protein; also SLP-65 and BASH) is a 50 kDa (predicted) cytoplasmic protein that runs anomalously at 65‑70 kDa in SDS‑PAGE. It is a B cell adapter molecule that, following phosphorylation by Syk, serves as a scaffold for assembly of macromolecular complexes that involve Grb2, Vav and PLC gamma. Tyrosine phosphorylation occurs at Tyr72/84/96/178/189. Human BLNK is 456 amino acids (aa) in length and it contains a Pro-rich region (aa 98‑260) and one SH2 domain (aa 346‑453). More than 10 potential splice variants have been detected and among these are four which show single in-frame deletions between aa 203‑225, 366‑417, 146‑417 and 211‑456, respectively. Over aa 226‑456, human BLNK shares 87% and 83% aa identity with canine and mouse BLNK, respectively.

Product Datasheets

Related Research Areas

• Adaptor Proteins
• B Cell Activation
• C-type Lectin Receptors