Home / Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 / Human beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody

Human beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody

Catalog #: MAB3960 Datasheet
Catalog # Availability Size / Price Qty
MAB3960
MAB3960-SP
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Human beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human B4GALT1 is observed
Source
Monoclonal Mouse IgG2B Clone # 485327
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2
Lys29-Cys397
Accession # Q9NY97
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 (Catalog # 3960-GT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2

beta 1,3-Linked GlcNAc residues are present in the backbone of various biologically important glycans which are involved in many essential biological functions such as keratan sulfate synthesis in corneal tissue (1). The addition of such residues are catalyzed by a family of beta 1,3‑N‑acetylglucosaminyltransferases, that includes at least eight members (1‑5).  All of them are type II Golgi resident transmembrane proteins and have high homology to the beta 1,3-galactosyltransferase family. beta 1,3‑N‑acetylglucosaminyltransferases 2 or beta 3GNT2 prefers the substrate lacto-N-neotetraose and is involved in the biosynthesis of poly-N-acetyllactosamine chains (6).

References
  1. Seko, A. and Yamashita, K. (2005) Glycobiology 15:943.
  2. Kataoka, K. and Huh, N.-H. (2002) Biochem. Biophys. Res. Commun. 294:843.
  3. Iwai,T. et al. (2002) J. Biol. Chem. 277:12802.
  4. Togayachi, A. et al. (2001) J. Biol. Chem. 276:22032.
  5. Sasaki, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14294.
  6. Shiraishi, N. et al. (2001) J. Biol. Chem. 276:3498.
Long Name
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyl-transferase 1
Entrez Gene IDs
10678 (Human); 53625 (Mouse)
Alternate Names
B3Galt6; B3GALT7; b3Gal-T7; B3GNT; B3GNT1; B3GN-T1; B3GNT2; B3GN-T2; B3GNT-2; Beta-1,3-galactosyltransferase 7; Beta-1,3-GalTase 7; Beta-1,3-Gn-T1; Beta-1,3-Gn-T2; Beta-1,3-N-acetylglucosaminyltransferase 1; Beta-1,3-N-acetylglucosaminyltransferase 2; beta-1,3-N-acetylglucosaminyltransferase bGnT-1; beta-1,3-N-acetylglucosaminyltransferase bGnT-2; Beta3GalT7; Beta3Gal-T7; BETA3GNT; Beta3gnt1; Beta3Gn-T1; Beta3Gn-T2; Beta-3-Gx-T7; BGnT-1; BGNT2; BGnT-2; EC 2.4.1.-; UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2

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