Human Axl PE-conjugated Antibody Summary
Met1-Pro440
Accession # AAA61243
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Axl in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Axl PE-conjugated Monoclonal Antibody (Catalog # FAB154P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of Axl in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with PE-conjugated Mouse Anti-Human Axl Monoclonal Antibody (Catalog # FAB154P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line. Axl knockout A431 human epithelial carcinoma cell line was stained with PE-conjugated Mouse Anti-Human Axl Monoclonal Antibody (Catalog # FAB154P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). No staining in the Axl knockout A431 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Axl
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1,2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.
- Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Holland, S. et al. (2005) Canc. Res. 65:9294.
Product Datasheets
Citations for Human Axl PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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MicroPET/CT Imaging of AXL Downregulation by HSP90 Inhibition in Triple-Negative Breast Cancer
Authors: Wanqin Wang, Jun Zhao, Xiaoxia Wen, Curtis Chun-Jen Lin, Junjie Li, Qian Huang et al.
Contrast Media & Molecular Imaging
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Peptidoglycan from Bacillus anthracis Inhibits Human Macrophage Efferocytosis in Part by Reducing Cell Surface Expression of MERTK and TIM-3
Authors: JS Mytych, Z Pan, C Lopez-Davi, N Redinger, C Lawrence, J Ziegler, N Popescu, JA James, AD Farris
bioRxiv : the preprint server for biology, 2023-05-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
AXL Knock-Out in SNU475 Hepatocellular Carcinoma Cells Provides Evidence for Lethal Effect Associated with G2 Arrest and Polyploidization
Authors: T Batur, A Argundogan, U Keles, Z Mutlu, H Alotaibi, S Senturk, M Ozturk
International Journal of Molecular Sciences, 2021-12-09;22(24):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Novel AXL-targeted agents overcome FLT3 inhibitor resistance in FLT3-ITD+ acute myeloid leukemia cells
Authors: Y Liu, J Wei, J Liu, W Ma, Y Duan, D Liu
Oncology Letters, 2021-03-18;21(5):397.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Reversal of pre-existing NGFR-driven tumor and immune therapy resistance
Authors: J Boshuizen, DW Vredevoogd, O Krijgsman, MA Ligtenberg, S Blankenste, B de Bruijn, DT Frederick, JCN Kenski, M Parren, M Brüggemann, MF Madu, EA Rozeman, JY Song, HM Horlings, CU Blank, ACJ van Akkooi, KT Flaherty, GM Boland, DS Peeper
Nat Commun, 2020-08-07;11(1):3946.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Constitutive Siglec-1 expression confers susceptibility to HIV-1 infection of human dendritic cell precursors
Authors: N Ruffin, E Gea-Mallor, F Brouiller, M Jouve, A Silvin, P See, CA Dutertre, F Ginhoux, P Benaroch
Proc. Natl. Acad. Sci. U.S.A., 2019-10-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Protein S and Gas6 induce efferocytosis of HIV-1-infected cells
Authors: BA Chua, JA Ngo, K Situ, CM Ramirez, H Nakano, K Morizono
Virology, 2018-01-02;515(0):176-190.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Axl is required for TGF-?2-induced dormancy of prostate cancer cells in the bone marrow
Sci Rep, 2016-11-07;6(0):36520.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Circulating levels of soluble MER in lupus reflect M2c activation of monocytes/macrophages, autoantibody specificities and disease activity.
Authors: Zizzo G, Guerrieri J, Dittman L, Merrill J, Cohen P
Arthritis Res Ther, 2013-01-01;15(6):R212.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
New insights into the phenotype of human dendritic cell populations.
Authors: Clark GJ et al.
Clin Transl Immunology
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