Human Axl Alexa Fluor® 488-conjugated Antibody Summary
Met1-Pro440
Accession # AAA61243
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Axl in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Axl Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB154G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of Axl in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Alexa Fluor® 488-conjugated Mouse Anti-Human Axl Monoclonal Antibody (Catalog # FAB154G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line. Axl knockout A431 human epithelial carcinoma cell line was stained with Alexa Fluor® 488-conjugated Mouse Anti-Human Axl Monoclonal Antibody (Catalog # FAB154G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). No staining in the Axl knockout A431 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Axl
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1,2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.
- Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Holland, S. et al. (2005) Canc. Res. 65:9294.
Product Datasheets
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
Citations for Human Axl Alexa Fluor® 488-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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An epigenetic switch regulates the ontogeny of AXL-positive/EGFR-TKi-resistant cells by modulating miR-335 expression
Authors: Polona Safaric Tepes, Debjani Pal, Trine Lindsted, Ingrid Ibarra, Amaia Lujambio, Vilma Jimenez Sabinina et al.
eLife
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AXL Expression on Homeostatic Resident Liver Macrophages Is Reduced in Cirrhosis Following GAS6 Production by Hepatic Stellate Cells
Authors: Oltin-Tiberiu Pop, Anne Geng, Emilio Flint, Arjuna Singanayagam, Caner Ercan, Lucia Possamai et al.
Cellular and Molecular Gastroenterology and Hepatology
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Transitional dendritic cells are distinct from conventional DC2 precursors and mediate proinflammatory antiviral responses
Authors: Sulczewski, FB;Maqueda-Alfaro, RA;Alcántara-Hernández, M;Perez, OA;Saravanan, S;Yun, TJ;Seong, D;Arroyo Hornero, R;Raquer-McKay, HM;Esteva, E;Lanzar, ZR;Leylek, RA;Adams, NM;Das, A;Rahman, AH;Gottfried-Blackmore, A;Reizis, B;Idoyaga, J;
Nature immunology
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Interactions between the NLRP3-Dependent IL-1beta and the Type I Interferon Pathways in Human Plasmacytoid Dendritic Cells
Authors: D Bencze, T Fekete, W Pfliegler, Á Szö?r, E Csoma, A Szántó, T Tarr, A Bácsi, L Kemény, Z Veréb, K Pázmándi
International Journal of Molecular Sciences, 2022-10-12;23(20):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity
Authors: R Leylek, M Alcántara-, JM Granja, M Chavez, K Perez, OR Diaz, R Li, AT Satpathy, HY Chang, J Idoyaga
Cell Rep, 2020-09-22;32(12):108180.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population
Authors: R Leylek, M Alcántara-, Z Lanzar, A Lüdtke, OA Perez, B Reizis, J Idoyaga
Cell Rep, 2019-12-10;29(11):3736-3750.e8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Sphingosine-1-Phosphate Receptor 1 Activity Promotes Tumor Growth by Amplifying VEGF-VEGFR2 Angiogenic Signaling
Authors: VA Balaji Rag, M Anwar, MZ Akhter, A Chavez, Y Mao, V Natarajan, S Lakshmikan, M Chrzanowsk, AZ Dudek, L Claesson-W, JK Kitajewski, KK Wary, AB Malik, D Mehta
Cell Rep, 2019-12-10;29(11):3472-3487.e4.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
TAM receptor-dependent regulation of SOCS3 and MAPKs contributes to proinflammatory cytokine downregulation following chronic NOD2 stimulation of human macrophages.
Authors: Zheng S, Hedl M, Abraham C
J Immunol, 2015-01-07;194(4):1928-37.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Transcriptional Basis of Mouse and Human Dendritic Cell Heterogeneity
Authors: Chrysothemis C. Brown, Herman Gudjonson, Yuri Pritykin, Deeksha Deep, Vincent-Philippe Lavallée, Alejandra Mendoza et al.
Cell
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