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Human ATAD2 Antibody

Catalog #: MAB9169 Datasheet
Catalog # Availability Size / Price Qty
MAB9169-100
MAB9169-SP
ATAD2 in MCF‑7 Human Cell Line.
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Human ATAD2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ATAD2 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 952947
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human ATAD2 synthetic peptide
Accession # Q6PL18
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry ATAD2 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

ATAD2 in MCF‑7 Human Cell Line. ATAD2 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human ATAD2 Monoclonal Antibody (Catalog # MAB9169) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATAD2

ATAD2/ANCCA/PRO2000 is an AAA (ATPases Associated with diverse cellular Activities) protein with two AAA domains and a bromodomain. ATAD2 is a coactivator in the regulatory process of ER alpha induction of target genes in breast cancer. Upon activation by estrogen, ATAD2 associates with ER alpha and ACTR and subsequently promotes expression of ER alpha target genes cyclin D1, c-myc and E2F1 in breast cancer cells.  ATAD2 also binds and hydrolyzes ATP, mediating hypermethylation of protein complexes. ATAD2 is reported to be a prognostic biomarker in endometrial, lung, ovarian and gastric cancers. The function of ATAD2 has also been reported in other carcinomas, including cervical, hepatocellular, colorectal and lung cancers.

Long Name
ATPase family, AAA domain containing 2
Entrez Gene IDs
29028 (Human); 70472 (Mouse); 314993 (Rat)
Alternate Names
AAA nuclear coregulator cancer-associated protein; ANCCA; ANCCAMGC131938; ATAD2; ATPase family AAA domain-containing protein 2; ATPase family, AAA domain containing 2; CT137; DKFZp667N1320; EC 3.6.1.3; MGC142216; MGC29843; MGC5254; PRO2000

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