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Human Artemin Antibody

Catalog #: MAB2589
2 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB2589
MAB2589-SP
Product Details
Citations (2)
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Human Artemin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Artemin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BIG-H3, rhCripto-1, recombinant mouse (rm) Cripto-1, recombinant Drosophila DPP, rhDPP-6, rhGDNF, rhLAP, rhLatent TGF-beta 1, rhTGF-beta 1.2, rhTGF‑ beta 3, rhLefty, rmLefty-1, rhMIS, rhNeurturin, rmNODAL, rhPersephin, recombinant amphibian TGF-beta 5, rhTGF-alpha or rhTGF-beta 2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 314908
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Artemin
Ala108-Gly220
Accession # Q5T4W7.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Artemin (Catalog # 2589-AR)
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Artemin

Human Artemin (ARTN; also known as enovin and neublastin) is a GDNF family ligand that is distantly related to the TGF-beta superfamily of molecules (1‑4). As such, it is synthesized as a preproprotein, and contains a variable length pre-, or signal sequence, plus a 68 amino acid (aa) proregion and a 113 aa mature segment (5‑7). Alternate splicing and start sites create signal sequences of 22, 30 and 39 aa, respectively. Following synthesis and proteolytic processing, mature ARTN is secreted as a presumably glycosylated, 28 kDa disulfide-linked homodimer that contains three intrachain disulfide bonds and the typical TGF-beta signature cysteine-knot motif (5, 7). In the mature region, human ARTN is 89% and 88% aa identical to rat (8) and mouse ARTN (5, 7), respectively. Cells known to express ARTN include Schwann cells (2) and embryonic vascular smooth muscle cells (9). Human ARTN is active on rodent cells (5). The receptor for ARTN has been identified as the ligand binding subunit GFR alpha -3 plus the signal transducing subunit, RET (1, 5). The GFR alpha -1/RET receptor complex has also been suggested to be a ligand binding unit for ARTN (2, 5). Evidence, however, suggests that the GFR alpha -1/RET complex plays no functional role in ARTN activity (10, 11). ARTN is known to be a chemoattractant for sympathetic neuron axons innervating the developing cardiovascular system (9). It also promotes sensory neuron survival and likely plays a role in the development of the peripheral nervous system (5). Finally, it has been reported to reverse neuropathic pain due to nerve injury, and to help resolve morphological changes associated with nerve damage (12).

References
  1. Airaksinen, M.S. and M. Saarma (2002) Nat. Rev. Neurosci. 3:383. 
  2. Saarma, M. (2000) Eur. J. Biochem. 267:6968. 
  3. Sariola, H. et al. (2003) J. Cell Sci. 116:3855. 
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787. 
  5. Baloh, R.H. et al. (1998) Neuron 21:1291. 
  6. Masure, S. et al. (1999) Eur. J. Biochem. 266:892. 
  7. Rosenblad, C. et al. (2000) Mol. Cell. Neurosci. 15:199.
  8. Stover, T. et al. (2000) Brain Res. Mol. Brain Res. 76:25.
  9. Honma, Y. et al. (2002) Neuron 35:267.
  10. Rakowicz, W.P. et al. (2002) J. Neurosci. 22:3953.
  11. Carmillo, P. et al. (2005) Biochemistry 44:2545.
  12. Gardell, L.R. et al. (2003) Nat. Med. 9:1383.
Entrez Gene IDs
9048 (Human); 11876 (Mouse)
Alternate Names
Artemin; ARTN; Enovin; EVN; EVNneurotrophic factor; NBN; Neublastin

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Citations for Human Artemin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Artemin-GFRalpha3 interactions partially contribute to acute inflammatory hypersensitivity.
    Authors: Thornton P, Hatcher J, Robinson I, Sargent B, Franzen B, Martino G, Kitching L, Glover C, Anderson D, Forsmo-Bruce H, Low C, Cusdin F, Dosanjh B, Williams W, Steffen A, Thompson S, Eklund M, Lloyd C, Chessell I, Hughes J
    Neurosci Lett, 2013-04-19;545(0):23-8.
    Species: Mouse
    Sample Types: Plasma
    Applications: Neutralization
  2. Nerve growth factor and artemin are paracrine mediators of pancreatic neuropathy in pancreatic adenocarcinoma.
    Authors: Ceyhan GO, Schafer KH, Kerscher AG, Rauch U, Demir IE, Kadihasanoglu M, Bohm C, Muller MW, Buchler MW, Giese NA, Erkan M, Friess H
    Ann. Surg., 2010-05-01;251(5):923-31.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Immunodepletion

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