Human Angiopoietin-like 3 Quantikine ELISA Kit
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Summary
Sample Values
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Human Angiopoietin-like 3 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.035 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human ANGPTL3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human ANGPTL3 in this assay. No medical histories were available for the donors used in this study.| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
| Serum (n=36) | 94.8 | 46.1-157 | 29.8 |
| EDTA plasma (n=36) | 108 | 55.8-172 | 29.7 |
| Heparin plasma (n=36) | 94.1 | 47.7-194 | 28.7 |
Cell Culture Supernates - HepG2 human hepatocellular carcinoma cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate until confluent. An aliquot of the cell culture supernate was removed, assayed for human ANGPTL3, and measured 2.62 ng/mL.
Product Summary
The Quantikine Human ANGPTL3 Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human ANGPTL3 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human ANGPTL3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ANGPTL3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human ANGPTL3.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.05 | 3.36 | 6.8 | 1.02 | 3.18 | 6.74 |
| Standard Deviation | 0.04 | 0.09 | 0.18 | 0.07 | 0.2 | 0.6 |
| CV% | 4.1 | 2.7 | 2.7 | 6.7 | 7.2 | 8.5 |
Recovery
The recovery of ANGPTL3 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 99-107 |
| EDTA Plasma (n=4) | 102 | 85-120 |
| Heparin Plasma (n=4) | 96 | 85-109 |
| Serum (n=4) | 96 | 81-115 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of ANGPTL3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Angiopoietin-like Protein 3/ANGPTL3
Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).
Entrez Gene IDs:
27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)
Alternate Names:
AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Citations for Human Angiopoietin-like 3 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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RNA interference targeting ANGPTL3 for triglyceride and cholesterol lowering: phase 1 basket trial cohorts
Authors: Watts, GF;Schwabe, C;Scott, R;Gladding, PA;Sullivan, D;Baker, J;Clifton, P;Hamilton, J;Given, B;Melquist, S;Zhou, R;Chang, T;San Martin, J;Gaudet, D;Goldberg, IJ;Knowles, JW;Hegele, RA;Ballantyne, CM;
Nature medicine
Species: Human
Sample Types: Serum
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RNAi-mediated rheostat for dynamic control of AAV-delivered transgenes
Authors: M Subramania, J McIninch, I Zlatev, MK Schlegel, C Kaittanis, T Nguyen, S Agarwal, T Racie, MA Alvarado, K Wassarman, TS Collins, T Chickering, CR Brown, K Schmidt, AB Castoreno, S Shulga-Mor, E Stamenova, K Buckowing, D Berman, JD Barry, A Bisbe, MA Maier, K Fitzgerald, V Jadhav
Nature Communications, 2023-04-08;14(1):1970.
Species: Human
Sample Types: Plasma
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Postprandial metabolism of apolipoproteins B48, B100, C-III and E in humans with APOC3 loss-of-function mutations
Authors: MR Taskinen, E Björnson, N Matikainen, S Söderlund, J Rämo, MM Ainola, A Hakkaraine, C Sihlbom, A Thorsell, L Andersson, PO Bergh, M Henricsson, S Romeo, M Adiels, S Ripatti, M Laakso, CJ Packard, J Borén
JCI Insight, 2022-10-10;0(0):.
Species: Human
Sample Types: Serum
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GALNT2 rs4846914 SNP Is Associated with Obesity, Atherogenic Lipid Traits, and ANGPTL3 Plasma Level
Authors: M Qaddoumi, P Hebbar, M Abu-Farha, A Al Somaly, M Melhem, F Al-Kayal, I AlKhairi, P Cherian, M Alanbaei, F Al-Mulla, J Abubaker, TA Thanaraj
Genes, 2022-07-04;13(7):.
Species: Human
Sample Types: Plasma
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ANGPTL3 Variants Associate with Lower Levels of Irisin and C-Peptide in a Cohort of Arab Individuals
Authors: M Alanbaei, M Abu-Farha, P Hebbar, M Melhem, BS Chandy, E Anoop, P Cherian, I Al-Khairi, F Alkayal, F Al-Mulla, J Abubaker, TA Thanaraj
Genes, 2021-05-17;12(5):.
Species: Human
Sample Types: Plasma
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Serum angiopoietin-like 3 levels are elevated in obese non diabetic men but are unaffected during an oral glucose tolerance test
Authors: MF Garcés, JD Buell-Acos, HA Rodríguez-, E Pulido-Sán, JJ Rincon-Ram, DC Moreno-Ord, R Franco-Veg, JS Roncancio-, AJ Burgos-Car, E Lacunza, JP Castaño, C Diéguez, R Nogueiras, E Sanchez, JE Caminos
Scientific Reports, 2020-12-03;10(1):21118.
Species: Human
Sample Types: Serum
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Profiling of selected angiogenesis-related genes in serous ovarian cancer patients
Authors: P Ku?, R Charkiewic, D Klasa-Mazu, T Milczek, B Mroczko, J Nikli?ski, P Lauda?ski
Adv Med Sci, 2017-02-21;62(1):116-120.
Species: Human
Sample Types: Cell Lysates
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Circulating angiopoietin-like protein 8 (ANGPTL8) and ANGPTL3 concentrations in relation to anthropometric and metabolic profiles in Korean children: a prospective cohort study.
Authors: Chung H, Lee M, Hwang S, Lee H, Yoo H, Seo J, Kim S, Kim N, Baik S, Choi D, Kim S, Choi K
Cardiovasc Diabetol, 2016-01-06;15(1):1.
Species: Human
Sample Types: Serum
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Human Angiopoietin-like 3 Quantikine ELISA Kit
By Anonymous
on 08/09/2023
Sample Tested: Serum
Human Angiopoietin-like 3 Quantikine ELISA Kit
By Anonymous
on 05/26/2022
Sample Tested: Serum
