Human Angiopoietin-2 Antibody Summary
Asp68-Phe496
Accession # O15123
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Angiopoietin‑2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Angiopoietin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF623) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Angiopoietin-2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Angiopoietin‑2 in Human Gastrointestinal Cancer Tissue. Angiopoietin-2 was detected in immersion fixed paraffin-embedded sections of human gastrointestinal cancer tissue using 15 µg/mL Goat Anti-Human Angiopoietin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF623) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Angiopoietin‑2 in Human Liver Cancer Tissue. Angiopoietin-2 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human Angiopoietin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF623) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human Angiopoietin-2 by Immunohistochemistry sAng-2 concentration positively relates with Ang-2 expression on the epithelia and MVD in cervical tissues.Representative immunohistochemical staining of Ang-1 (A), Ang-2 (B) and CD34 (C) in 25 cervical cancer tissue specimens and 10 normal controls. Black arrows denote positively stained epithelial cells, whereas red arrows denote positively staining endothelial cells, all appearing brown. Scale bar, 20 µm. (D) sAng-2 is significantly higher in the patients with positive Ang-2 expression on cervix epithelia than those with negative Ang-2 expression. The scatter diagrams show the correlations of sAng-1 (E), sAng-2 (F) and sAng-1/ sAng-2 ratio (G) to MVD in the 35 cervical tissue specimens. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28584715), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-2
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids (aa) in length. It contains one coiled-coil domain (aa 166-248) that mediates multimerization, and a C-terminal fibrinogen-like domain (aa 275-495) that mediates receptor binding. Under reducing conditions, secreted monomeric Ang-2 is 65-66 kDa in size. Under nonreducing conditions, both natural and recombinant Ang-2 form 140 kDa dimers, 200 kDa trimers, and 250-300 kDa tetramers and pentamers. Alternate splicing generates a short isoform that lacks 52 amino acids (aa) preceding the coiled-coil domain. Mature human Ang-2 shares 86% aa sequence identity with mouse and rat Ang-2. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel-Palade bodies. Both Ang-2 and the related Angiopoietin-1 (Ang-1) are ligands for the receptor tyrosine kinase Tie-2. While Ang-1 is a potent Tie-2 agonist, Ang-2 may act as either a Tie-2 antagonist or agonist, depending upon its state of multimerization. The higher the order of oligomer, the more effective Ang-2 becomes as a Tie-2 agonist. The short isoform appears to block the binding of either Ang-1 or full-length Ang-2 to Tie-2. Ang-2 functions as a pro-angiogenic factor, although it can also induce EC death and vessel regression. Upon its release from quiescent EC, it regulates vascular remodeling by promoting EC survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells. Ang-2 is required for postnatal vascular remodeling, and it cooperates with Ang-1 during lymphatic vessel development. It mediates the upregulation of ICAM-1 and VCAM-1 on EC, which facilitates the adhesion of leukocytes during inflammation. Ang-2 is upregulated in both the endothelium and tumor cells of several cancers as well as in ischemic tissue. Its direct interaction with Integrins promotes tumor cell invasion. Ang-2 also promotes the neuronal differentiation and migration of subventricular zone progenitor cells.
Product Datasheets
Citations for Human Angiopoietin-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Endothelial angiopoietin-2 overexpression in explanted livers identifies subjects at higher risk of recurrence of hepatocellular carcinoma after liver transplantation
Authors: Simone Lasagni, Filippo Leonardi, Alessandra Pivetti, Lorenza Di Marco, Federico Ravaioli, Matteo Serenari et al.
Frontiers in Oncology
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Efficacy of a Bispecific Antibody Co-Targeting VEGFA and Ang-2 in Combination with Chemotherapy in a Chemoresistant Colorectal Carcinoma Xenograft Model
Authors: Thomas Mueller, Juana Freystein, Henrike Lucas, Hans-Joachim Schmoll
Molecules
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Angiopoietin-2 interferes with anti-VEGFR2- induced vessel normalization and survival benefit in mice bearing gliomas
Authors: Sung-Suk Chae, Walid S. Kamoun, Christian T. Farrar, Nathaniel D. Kirkpatrick, Elisabeth Niemeyer, Annemarie M.A. de Graaf et al.
Clinical Cancer Research
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Lowering the increased intracellular pH of human-induced pluripotent stem cell-derived endothelial cells induces formation of mature Weibel-Palade bodies
Authors: Gesa L. Tiemeier, Rozemarijn de Koning, Gangqi Wang, Sarantos Kostidis, Rosalie G. J. Rietjens, Wendy M. P. J. Sol et al.
Stem Cells Translational Medicine
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Modulation of Angiopoietin 2 release from endothelial cells and angiogenesis by the synaptic protein Neuroligin 2
Authors: Margherita Pergolizzi, Laura Bizzozero, Elena Riccitelli, Davide Pascal, Anna Valeria Samarelli, Federico Bussolino et al.
Biochemical and Biophysical Research Communications
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Abnormal angiogenesis in blood outgrowth endothelial cells derived from von Willebrand disease patients
Authors: Soundarya N. Selvam, Lara J. Casey, Mackenzie L. Bowman, Lindsey G. Hawke, Avery J. Longmore, Jeffrey Mewburn et al.
Blood Coagulation & Fibrinolysis
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Yes-associated protein regulates endothelial cell contact-mediated expression of angiopoietin-2
Authors: Hyun-Jung Choi, Haiying Zhang, Hongryeol Park, Kyu-Sung Choi, Heon-Woo Lee, Vijayendra Agrawal et al.
Nature Communications
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A single-cell atlas of the normal and malformed human brain vasculature
Authors: Ethan A. Winkler, Chang N. Kim, Jayden M. Ross, Joseph H. Garcia, Eugene Gil, Irene Oh et al.
Science
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Differential Impact of Tumor Endothelial Angiopoietin-2 and Podoplanin in Lymphatic Endothelial Cells on HCC Outcomes with Tyrosine Kinase Inhibitor Treatment According to Sex
Authors: Lasagni, S;Critelli, RM;Milosa, F;Saltini, D;Schepis, F;Romanzi, A;Dituri, F;Serino, G;Di Marco, L;Pivetti, A;Scianò, F;Giannelli, G;Villa, E;
Biomedicines
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
The possible dual role of Ang-2 in the prognosis of pancreatic cancer
Authors: Roos-Mattila, M;Kaprio, T;Mustonen, H;Hagström, J;Saharinen, P;Haglund, C;Seppänen, H;
Scientific reports
Species: Human
Sample Types:
Applications: IHC -
MEK inhibition reduced vascular tumor growth and coagulopathy in a mouse model with hyperactive GNAQ
Authors: S Schrenk, LJ Bischoff, J Goines, Y Cai, S Vemaraju, Y Odaka, SR Good, JS Palumbo, S Szabo, D Reynaud, CD Van Raamsd, RA Lang, E Boscolo
Nature Communications, 2023-04-06;14(1):1929.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Angiopoietin-2 Inhibition of Thrombomodulin-Mediated Anticoagulation-A Novel Mechanism That May Contribute to Hypercoagulation in Critically Ill COVID-19 Patients
Authors: M Hultström, K Fromell, A Larsson, B Persson, B Nilsson, SE Quaggin, C Betsholtz, R Frithiof, M Lipcsey, M Jeansson
Biomedicines, 2022-06-06;10(6):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain
Authors: YG Choe, JH Yoon, J Joo, B Kim, SP Hong, GY Koh, DS Lee, WY Oh, Y Jeong
Frontiers in Cellular Neuroscience, 2022-03-11;16(0):848764.
Species: Mouse
Sample Types: Protein Lysates
Applications: Western Blot -
ADAM9 promotes lung cancer progression through vascular remodeling by VEGFA, ANGPT2, and PLAT
Authors: CY Lin, CF Cho, ST Bai, JP Liu, TT Kuo, LJ Wang, YS Lin, CC Lin, LC Lai, TP Lu, CY Hsieh, CN Chu, DC Cheng, YP Sher
Sci Rep, 2017-11-08;7(1):15108.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis
Authors: J Retzlaff, K Thamm, CC Ghosh, W Ziegler, H Haller, SM Parikh, S David
Sci Rep, 2017-03-09;7(0):44113.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation.
Authors: Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, Liang X, Wang Z, Yuan Q, Vortmeyer A, Toomre D, Fuh G, Yan M, Kluger M, Wu D, Min W
Nat Med, 2016-08-22;22(9):1033-1042.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Association of Angiopoietin-2 and Ki-67 Expression with Vascular Density and Sunitinib Response in Metastatic Renal Cell Carcinoma
Authors: J Rautiola, A Lampinen, T Mirtti, A Ristimäki, H Joensuu, P Bono, P Saharinen
PLoS ONE, 2016-04-21;11(4):e0153745.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Endothelial destabilization by angiopoietin-2 via integrin beta1 activation.
Authors: Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P
Nat Commun, 2015-01-30;6(0):5962.
Species: Human
Sample Types: Whole Cells
Applications: ICC, IHC -
Angiopoietin-2 and biliary diseases: elevated serum, but not bile levels are associated with cholangiocarcinoma.
Authors: Voigtlander T, David S, Thamm K, Schlue J, Metzger J, Manns M, Lankisch T
PLoS ONE, 2014-05-13;9(5):e97046.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.
Authors: Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perriere N, Guillevic O, Couraud P, Uzan G
PLoS ONE, 2014-01-02;9(1):e84179.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Angiopoietin-2 secretion by endothelial cell exosomes: regulation by the phosphatidylinositol 3-kinase (PI3K)/Akt/endothelial nitric oxide synthase (eNOS) and syndecan-4/syntenin pathways.
Authors: Ju, Rong, Zhuang, Zhen W, Zhang, Jiasheng, Lanahan, Anthony, Kyriakides, Themis, Sessa, William, Simons, Michael
J Biol Chem, 2013-11-14;289(1):510-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Norrin promotes vascular regrowth after oxygen-induced retinal vessel loss and suppresses retinopathy in mice.
Authors: Ohlmann A, Seitz R, Braunger B, Seitz D, Bosl MR, Tamm ER
J. Neurosci., 2010-01-06;30(1):183-93.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Angiopoietin-2 levels are associated with disease progression in metastatic malignant melanoma.
Authors: Helfrich I, Edler L, Sucker A, Thomas M, Christian S, Schadendorf D, Augustin HG
Clin. Cancer Res., 2009-02-15;15(4):1384-92.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Dynamic angiopoietin-2 assessment predicts survival and chronic course in hospitalized patients with COVID-19
Authors: Erica Villa, Rosina Critelli, Simone Lasagni, Alessandra Melegari, Angela Curatolo, Ciro Celsa et al.
Blood Advances
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Modulation of the Permeability-Inducing Factor Angiopoietin-2 Through Bifonazole in Systemic Inflammation
Authors: Thorben Pape, Temitayo Opemipo Idowu, Valerie Maritta Etzrodt, Klaus Stahl, Benjamin Seeliger, Hermann Haller et al.
Shock
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YAP and TAZ maintain PROX1 expression in the developing lymphatic and lymphovenous valves in response to VEGF-C signaling
Authors: Boksik Cha, Yen-Chun Ho, Xin Geng, Md. Riaj Mahamud, Lijuan Chen, Yeunhee Kim et al.
Development
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GATA2 controls lymphatic endothelial cell junctional integrity and lymphovenous valve morphogenesis through miR-126
Authors: Md. Riaj Mahamud, Xin Geng, Yen-Chun Ho, Boksik Cha, Yuenhee Kim, Jing Ma et al.
Development
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Presence of sst5TMD4, a truncated splice variant of the somatostatin receptor subtype 5, is associated to features of increased aggressiveness in pancreatic neuroendocrine tumors.
Authors: Sampedro-Nunez M, Luque RM, Ramos-Levi AM et al.
Oncotarget
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