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Human Angiopoietin-2 Antibody

Catalog #: MAB0983
8 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB0983-SP
MAB0983-100
MAB0983-500
Detection of Human Angiopoietin‑2 by Western Blot.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Angiopoietin-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Angiopoietin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) Ang-1, rhAng-4, rhAng-X, or rmANGPTL3.
Source
Monoclonal Mouse IgG2B Clone # 180102
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-2
Asp68-Phe496
Accession # O15123
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Angiopoietin-2 antibody by Western Blot. View Larger

Detection of Human Angiopoietin‑2 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HepG2 human hepatocellular carcinoma cell line, human placenta tissue, human ovary tissue, and human uterus tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Angiopoietin-2 Monoclonal Antibody (Catalog # MAB0983) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Angiopoietin-2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-2

Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation (1, 2). Mature Ang-2 is 478 amino acids (aa) in length. It contains one coiled-coil domain (aa 166 - 248) that mediates multimerization, and a C-terminal fibrinogen-like domain (aa 275 - 495) that mediates receptor binding. Under reducing conditions, secreted monomeric Ang-2 is 65 - 66 kDa in size. Under nonreducing conditions, both natural and recombinant Ang-2 form 140 kDa dimers, 200 kDa trimers, and 250 - 300 kDa tetramers and pentamers (3 - 6). Alternate splicing generates a short isoform that lacks 52 amino acids preceding the coiled-coil domain (4). Mature human Ang-2 shares 86% aa sequence identity with mouse and rat Ang-2. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus (3). It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel-Palade bodies (1, 3, 7). Both Ang-2 and the related Angiopoietin-1 (Ang-1) are ligands for the receptor tyrosine kinase Tie-2 (2). While Ang‑1 is a potent Tie-2 agonist, Ang-2 may act as either a Tie-2 antagonist or agonist, depending upon its state of multimerization. The higher the order of oligomer, the more effective Ang‑2 becomes as a Tie-2 agonist (3, 8 - 11). The short isoform appears to block the binding of either Ang-1 or full-length Ang-2 to Tie-2 (4). Ang-2 functions as a pro-angiogenic factor, although it can also induce EC death and vessel regression (12, 13). Upon its release from quiescent EC, it regulates vascular remodeling by promoting EC survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells (8, 13, 14). Ang-2 is required for postnatal vascular remodeling, and it cooperates with Ang-1 during lymphatic vessel development (7, 15). It mediates the upregulation of ICAM-1 and VCAM-1 on EC, which facilitates the adhesion of leukocytes during inflammation (16). Ang-2 is upregulated in both the endothelium and tumor cells of several cancers as well as in ischemic tissue (17 - 20). Its direct interaction with Integrins promotes tumor cell invasion (21, 22). Ang-2 also promotes the neuronal differentiation and migration of subventricular zone progenitor cells (20).

References
  1. Augustin, H.G. et al. (2009) Nat. Rev. Mol. Cell Biol. 10:165.
  2. Murdoch, C. et al. (2007) J. Immunol. 178:7405.
  3. Maisonpierre, P.C. et al. (1997) Science 27:55.
  4. Kim, I. et al. (2000) J. Biol. Chem. 275:18550.
  5. Procopio, W.N. et al. (1999) J. Biol. Chem. 274:30196.
  6. Kim, K-T. et al. (2005) J. Biol. Chem. 280:20126.
  7. Gale, N.W. et al. (2002) Dev. Cell 3:411.
  8. Yuan, H.T. et al. (2009) Mol. Cell. Biol. 29:2011.
  9. Falcon, B.L. et al. (2009) Am. J. Pathol. 175:2159.
  10. Kim, H-Z. et al. (2009) Biochim. Biophys. Acta 1793:772.
  11. Kim, I. et al. (2001) Cardiovasc. Res. 49:872.
  12. Lobov, I.B. et al. (2002) Proc. Natl. Acad. Sci. 99:11205.
  13. Cao, Y. et al. (2007) Cancer Res. 67:3835.
  14. Nasarre, P. et al. (2009) Cancer Res. 69:1324.
  15. Dellinger, M. et al. (2008) Dev. Biol. 319:309.
  16. Fiedler, U. et al. (2006) Nat. Med. 12:235.
  17. Koga, K. et al. (2001) Cancer Res. 61:6248.
  18. Etoh, T. et al. (2001) Cancer Res. 61:2145.
  19. Tressel, S.L. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1989.
  20. Liu, X.S. et al. (2009) J. Biol. Chem. 284:22680.
  21. Hu, B. et al. (2006) Cancer Res. 66:775.
  22. Imanishi, Y. et al. (2007) Cancer Res. 67:4254.
Entrez Gene IDs
285 (Human); 11601 (Mouse); 89805 (Rat); 396730 (Porcine); 101925495 (Cynomolgus Monkey)
Alternate Names
AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand

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Citations for Human Angiopoietin-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Angiopoietin-2 plasma dosage predicts time to first treatment and overall survival in chronic lymphocytic leukemia.
    Authors: Maffei R, Martinelli S, Santachiara R
    Blood, 2010-04-09;116(4):584-92.
  2. Endothelial pyruvate kinase M2 maintains vascular integrity
    Authors: B Kim, C Jang, H Dharaneesw, J Li, M Bhide, S Yang, K Li, Z Arany
    J. Clin. Invest., 2018-09-17;0(0):.
  3. Shear stress-regulated miR-27b controls pericyte recruitment by repressing SEMA6A and SEMA6D
    Authors: S Demolli, A Doddaballa, K Devraj, K Stark, Y Manavski, A Eckart, CM Zehendner, T Lucas, T Korff, M Hecker, S Massberg, S Liebner, D Kaluza, RA Boon, S Dimmeler
    Cardiovasc. Res., 2017-05-01;113(6):681-691.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Yes-associated protein regulates endothelial cell contact-mediated expression of angiopoietin-2
    Authors: Hyun-Jung Choi, Haiying Zhang, Hongryeol Park, Kyu-Sung Choi, Heon-Woo Lee, Vijayendra Agrawal et al.
    Nature Communications
  5. Physical contact with endothelial cells through beta 1- and beta 2- integrins rescues chronic lymphocytic leukemia cells from spontaneous and drug-induced apoptosis and induces a peculiar gene expression profile in leukemic cells
    Authors: Rossana Maffei, Stefania Fiorcari, Jenny Bulgarelli, Silvia Martinelli, Ilaria Castelli, Silvia Deaglio et al.
    Haematologica
  6. Regulation of angiopoietin expression by bacterial lipopolysaccharide.
    Authors: Mofarrahi M, Nouh T, Qureshi S, Guillot L, Mayaki D, Hussain SN
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008-02-29;294(5):L955-63.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Developmental differences in the responses of IL-6 and IL-13 transgenic mice exposed to hyperoxia.
    Authors: Choo-Wing R, Nedrelow JH, Homer RJ, Elias JA, Bhandari V
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007-03-30;293(1):L142-50.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Differential expression of angiopoietin-1, angiopoietin-2, and Tie receptors in placentas from pregnancies complicated by placenta accreta.
    Authors: Tseng JJ, Hsu SL, Ho ES, Hsieh YT, Wen MC, Chou MM
    Am. J. Obstet. Gynecol., 2006-02-01;194(2):564-71.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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