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Human Angiopoietin-1 Antibody

Catalog #: MAB9231
3 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB9231-SP
MAB9231-100
MAB9231-500
Product Details
Citations (3)
FAQs
Supplemental Products
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Angiopoietin-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Angiopoietin-1 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) Ang‑2, rhAng‑4, rmANGPTL3, or rhANGPTL7.
Source
Monoclonal Mouse IgG2B Clone # 171733
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-1
Ser20-Phe498
Accession # Q15389
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Angiopoietin‑1 (Catalog # 923-AN)

Human Angiopoietin-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Angiopoietin‑1 Biotinylated Antibody (Catalog # BAF923)

Standard: Recombinant Human Angiopoietin-1 Protein (Catalog # 923-AN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-1

Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are two closely related secreted ligands which bind with similar affinity to Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature (1). Ang-1 cDNA encodes a 498 amino acid (aa) residue precursor protein that contains a coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-1 shares approximately 97% and 60% aa sequence identity with mouse Ang‑1 and human Ang‑2, respectively (1, 2). Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization (1, 3, 4). Ang-2 has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context (5).

References
  1. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
  2. Davis, S. et al. (1996) Cell 87:1161.
  3. Witzenbichler, B. et al. (1998) J. Biol. Chem. 273:18514.
  4. Papapetropoulos, A. et al. (1999) Lab. Inest. 79:213.
  5. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.
Entrez Gene IDs
284 (Human); 11600 (Mouse)
Alternate Names
AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003

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Citations for Human Angiopoietin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Non-neutralizing antibodies increase endogenous circulating Ang1 levels
    Authors: Zheng C, Toth J, Bigwarfe T et al.
    MAbs
  2. Angiopoietin-2 levels are elevated in exudative pleural effusions.
    Authors: Kalomenidis I, Kollintza A, Sigala I, Papapetropoulos A, Papiris S, Light RW, Roussos C
    Chest, 2006-05-01;129(5):1259-66.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  3. Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
    Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
    Eur. J. Clin. Invest., 2003-10-01;33(10):883-90.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development

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