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Human AMPK alpha 2 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB2850R Datasheet
Catalog # Availability Size / Price Qty
FAB2850R-100UG
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Human AMPK alpha 2 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human AMPK alpha 2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human AMPK alpha 1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 847123
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human AMPKα2
Phe340-Arg552
Accession # P54646
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: AMPK alpha 2

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and δ subunits. The alpha subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinase LKB1. Active AMPK down-regulates anabolic pathways such as fatty acid and cholesterol synthesis, and up-regulates catabolic pathways such as glycolysis and fatty acid oxidation.

Long Name
AMP-activated Protein Kinase alpha
Entrez Gene IDs
5563 (Human); 108079 (Mouse); 78975 (Rat)
Alternate Names
5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK alpha 2; AMPK subunit alpha-2; AMPK2; AMPK5'-AMP-activated protein kinase, catalytic alpha-2 chain; AMPK-alpha-2 chain; EC 2.7.11; EC 2.7.11.1; PRKAA; PRKAA2; protein kinase, AMP-activated, alpha 2 catalytic subunit

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