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Human Aggrecan G1-IGD-G2 Domains Antibody

Catalog #: AF1220
19 Citations Datasheet
Catalog # Availability Size / Price Qty
AF1220
AF1220-SP
Aggrecan in Rat Mesenchymal Stem Cells.
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Citations (19)
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Human Aggrecan G1-IGD-G2 Domains Antibody Summary

Species Reactivity
Human
Specificity
Detects human Aggrecan in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Aggrecan
Val20-Arg675
Accession # NP_037359
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Aggrecan G1-IGD-G2 Domains (Catalog # 1220-PG)
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Aggrecan antibody in Rat Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

Aggrecan in Rat Mesenchymal Stem Cells. Aggrecan was detected in immersion fixed rat mesenchymal stem cells differentiated to chondrocytes using Goat Anti-Human Aggrecan G1-IGD-G2 Domains Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1220) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju-gated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Aggrecan antibody in Human Cartilage by Immunohistochemistry (IHC-P). View Larger

Aggrecan in Human Cartilage. Aggrecan was detected in immersion fixed paraffin-embedded sections of human cartilage using 15 µg/mL Goat Anti-Human Aggrecan G1-IGD-G2 Domains Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1220) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry/ Immunofluorescence Detection of Porcine Aggrecan by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Porcine Aggrecan by Immunocytochemistry/ Immunofluorescence In vitro differentiation capacity of young and old BM-MSCs: (A) Representative micrographs from each experimental group showing morphological change after 21 days of differentiation. (B) Results of adipogenic, osteogenic and chondrogenic differentiation. Representative micrographs showing anti-FABP4, Oil Red O, anti-osteocalcin, Alizarin Red-S and anti-aggrecan staining in cultured BM-MSCs from each experimental group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/21992089), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aggrecan

Aggrecan, also known as aggrecan 1, chondroitin sulfate proteoglycan, and large aggregating proteoglycan, is encoded by the AGC1 gene with gene aliases of SEDK; CSPG1; MSK16; CSPGCP. As the key component of the cartilage extracellular matrix, aggrecan hydrates the collagen network and provides cartilage with its properties of compressibility and elasticity. Maintenance of aggrecan content is therefore critical to the function of the tissue and aggrecan degradation is an important factor in the erosion of articular cartilage in arthritic diseases (2). The deduced amino acid sequence of human aggrecan core protein consists of 2415 resides and predicts a signal peptide and domains of G1, IGD, G2, KS, CS-1, CS-2, and G3 (3). Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan and also contain link domains. The third globular domain, G3, corresponds to the C-terminus. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains are between G2 and G3. With KS and CS attached to the 250 kDa core protein, aggrecan monomers exist as a 1,000-2,000 kDa molecule. In addition, aggrecan monomers interact with hyaluronan through their G1 domain, resulting in larger aggregates containing 10-100 aggrecan monomers on a hyaluronan backbone (2).

Aggrecan can be cleaved by MMPs and ADAMTSs at the Asn360-Phe361 and Glu392-Ala393 bond in the IGD (residues are numbered based on Accession # NP_037359), respectively (2). Inhibition of ADATMS4 and ADAMTS5 cleavage prevents aggrecan degradation in osteoarthritic cartilage, while mice with aggrecan resistant to MMP cleavage do not accumulate aggrean and develop normally (2, 4). Consisting of the G1, IGD and G2 domains, rhAggrecan can be used as a protein substrate for MMPs and ADAMTSs and in binding assays involving hyaluronan.

References
  1. Doege. K.J. et al. (1991) J. Biol. Chem. 266:894.
  2. Malfait, A-M. et al. (2002) J. Biol. Chem. 277:22201.
  3. Caterson, B. et al. (2000) Matrix Biol. 19:333.
  4. Little, C.B. et al. (2005) Mol. Cel. Biol. 25:3388.
Entrez Gene IDs
176 (Human)
Alternate Names
ACAN; AGC1; AGC1SEDK; aggrecan core protein; Aggrecan; Cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan core protein 1; CSPCP; CSPG1; CSPG1aggrecan 1; CSPGCP; large aggregating proteoglycan; MSK16; MSK16AGCAN; SEDK

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Citations for Human Aggrecan G1-IGD-G2 Domains Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Single-cell atlas unveils cellular heterogeneity and novel markers in human neonatal and adult intervertebral discs
    Authors: Wensen Jiang, Juliane D. Glaeser, Khosrowdad Salehi, Giselle Kaneda, Pranav Mathkar, Anton Wagner et al.
    iScience
  2. Fibrillin-2 is a key mediator of smooth muscle extracellular matrix homeostasis during mouse tracheal tubulogenesis
    Authors: Wenguang Yin, Hyun-Taek Kim, ShengPeng Wang, Felix Gunawan, Rui Li, Carmen Buettner et al.
    European Respiratory Journal
  3. Cholinergic-like neurons and cerebral spheroids bearing the PSEN1 p.Ile416Thr variant mirror Alzheimer's disease neuropathology
    Authors: Gomez-Sequeda, N;Mendivil-Perez, M;Jimenez-Del-Rio, M;Lopera, F;Velez-Pardo, C;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Directed differentiation of human iPSCs into mesenchymal lineages by optogenetic control of TGF-? signaling
    Authors: Wu, JY;Yeager, K;Tavakol, DN;Morsink, M;Wang, B;Soni, RK;Hung, CT;Vunjak-Novakovic, G;
    Cell reports
    Species: Human
    Sample Types: Spheroids
    Applications: IHC
  5. Characterization and Advancement of an Evaluation Method for the Treatment of Spontaneous Osteoarthritis in STR/ort Mice: GRGDS Peptides as a Potential Treatment for Osteoarthritis
    Authors: Chen, MF;Hu, CC;Hsu, YH;Chiu, YT;Chen, KL;Ueng, SWN;Chang, Y;
    Biomedicines
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Hyaluronic acid hydrogels support to generate integrated bone formation through endochondral ossification in vivo using mesenchymal stem cells
    Authors: S Yamazaki, R Hirayama, Y Ikeda, S Iseki, T Yoda, MA Ikeda
    PLoS ONE, 2023-02-02;18(2):e0281345.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  7. Does TBC1D4 (AS160) or TBC1D1 Deficiency Affect the Expression of Fatty Acid Handling Proteins in the Adipocytes Differentiated from Human Adipose-Derived Mesenchymal Stem Cells (ADMSCs) Obtained from Subcutaneous and Visceral Fat Depots?
    Authors: A Mik?osz, B ?ukaszuk, E Supruniuk, K Grubczak, M Moniuszko, B Choroma?sk, P My?liwiec, A Chabowski
    Cells, 2021-06-16;10(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  8. Stabilization of heterochromatin by CLOCK promotes stem cell rejuvenation and cartilage regeneration
    Authors: C Liang, Z Liu, M Song, W Li, Z Wu, Z Wang, Q Wang, S Wang, K Yan, L Sun, T Hishida, Y Cai, JCI Belmonte, P Guillen, P Chan, Q Zhou, W Zhang, J Qu, GH Liu
    Cell Res., 2020-07-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases
    Authors: AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano
    Sci Rep, 2020-07-31;10(1):12912.
    Species: Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  10. Human mesenchymal stem cells are resistant to UV-B irradiation
    Authors: R Lopez Pere, J Brauer, A Rühle, T Trinh, S Sisombath, P Wuchter, AL Grosu, J Debus, R Saffrich, PE Huber, NH Nicolay
    Sci Rep, 2019-12-27;9(1):20000.
    Species: Human
    Sample Types: Spheroid
    Applications: IHC-Fr
  11. Newly defined ABCB5+ dermal mesenchymal stem cells promote healing of chronic iron overload wounds via secretion of interleukin-1 receptor antagonist
    Authors: S Vander Bek, JC de Vries, B Meier-Schi, P Meyer, D Jiang, A Sindrilaru, FF Ferreira, A Hainzl, S Schatz, J Muschhamme, NJ Scheurmann, P Kampilafko, AM Seitz, L Dürselen, A Ignatius, MA Kluth, C Ganss, M Wlaschek, K Singh, P Maity, NY Frank, MH Frank, K Scharffett
    Stem Cells, 2019-05-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Effects of sesamin on chondroitin sulfate proteoglycan synthesis induced by interleukin-1beta in human chondrocytes
    Authors: W Srisuthtay, D Pruksakorn, P Kongtawele, P Pothacharo
    BMC Complement Altern Med, 2017-05-31;17(1):286.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Identification of multipotent stem cells in human brain tissue following stroke
    Authors: K Tatebayash, Y Tanaka, A Nakano-Doi, R Sakuma, S Kamachi, M Shirakawa, K Uchida, H Kageyama, T Takagi, S Yoshimura, T Matsuyama, T Nakagomi
    Stem Cells Dev, 2017-04-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. MSC from fetal and adult lungs possess lung-specific properties compared to bone marrow-derived MSC.
    Authors: Rolandsson Enes S, Andersson Sjoland A, Skog I, Hansson L, Larsson H, Le Blanc K, Eriksson L, Bjermer L, Scheding S, Westergren-Thorsson G
    Sci Rep, 2016-07-06;6(0):29160.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  15. Gene expression profile analysis of human mesenchymal stem cells from herniated and degenerated intervertebral discs reveals different expression of osteopontin.
    Authors: Marfia G, Navone S, Di Vito C, Tabano S, Giammattei L, Di Cristofori A, Gualtierotti R, Tremolada C, Zavanone M, Caroli M, Torchia F, Miozzo M, Rampini P, Riboni L, Campanella R
    Stem Cells Dev, 2014-10-29;24(3):320-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Human embryonic stem cell-derived mesenchymal stroma cells (hES-MSCs) engraft in vivo and support hematopoiesis without suppressing immune function: implications for off-the shelf ES-MSC therapies.
    Authors: Li O, Tormin A, Sundberg B, Hyllner J, Le Blanc K, Scheding S
    PLoS ONE, 2013-01-29;8(1):e55319.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  17. Age-related changes in rat bone-marrow mesenchymal stem cell plasticity.
    Authors: Asumda FZ, Chase PB
    BMC Cell Biol., 2011-10-12;12(0):44.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  18. Effect of functionalised fluorescence-labelled nanoparticles on mesenchymal stem cell differentiation.
    Authors: Tautzenberger A, Lorenz S, Kreja L, Zeller A, Musyanovych A, Schrezenmeier H, Landfester K, Mailander V, Ignatius A
    Biomaterials, 2009-12-11;31(8):2064-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. Safety and efficacy of human juvenile chondrocyte-derived cell sheets for osteochondral defect treatment
    Authors: Makoto Kondo, Sumako Kameishi, Kyungsook Kim, Nicolas F. Metzler, Travis G. Maak, Douglas T. Hutchinson et al.
    npj Regenerative Medicine

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