Human AG-2/AGR2 Antibody Summary
Arg21-Leu175
Accession # O95994
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human AG-2/AGR2 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and human small intestine tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human AG-2/AGR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6068) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AG-2/AGR2 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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AG-2/AGR2 in Human Breast Cancer Tissue. AG-2/AGR2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human AG-2/AGR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6068) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AG-2/AGR2
AG-2 (Anterior gradient protein 2; also AGR2, GOB4 and HPC8) is an 18‑21 kDa member of the PDI family of enzymes. It is expressed in secretory cell types, such as prostate epithelium, small intestine goblet, Paneth and enteroendocrine cells, and multiple carcinoma cell types. Although AG-2 possesses a signal sequence, it appears to be rarely secreted, instead being found in the ER due the presence of a KTEL ER retention signal at its C-terminus. AG-2 forms transient disulfide linkages with molecules destined for secretion, possibly aiding protein folding. Mature human AG-2 is 155 amino acids (aa) in length (aa 21‑175). Cys81 is presumed to participate in intermolecular bond formation. There is one potential alternative start site 20 aa upstream of the standard start site. Over aa 21‑175, human AG-2 shares 94% aa identity with mouse AG-2.
Product Datasheets
Citations for Human AG-2/AGR2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Slowed Intestinal Transit Induced by Less Mucus in Intestinal Goblet Cell Piezo1-Deficient Mice through Impaired Epithelial Homeostasis
Authors: Fang, F;Liu, Y;Xiong, Y;Li, X;Li, G;Jiang, Y;Hou, X;Song, J;
International journal of molecular sciences
Species: Transgenic Mouse
Sample Types: Organoid, Whole Tissue
Applications: IHC -
Activation of goblet cell Piezo1 alleviates mucus barrier damage in mice exposed to WAS by inhibiting H3K9me3 modification
Authors: Y Xu, Y Xiong, Y Liu, G Li, T Bai, G Zheng, X Hou, J Song
Cell & bioscience, 2023-01-12;13(1):7.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Reprogrammed fecal and mucosa-associated intestinal microbiota and weakened mucus layer in intestinal goblet cell- specific Piezo1-deficient mice
Authors: Ying Liu, Feifei Fang, Yilin Xiong, Jiandi Wu, Xueyan Li, Gangping Li et al.
Frontiers in Cellular and Infection Microbiology
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