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Human Adiponectin/Acrp30 Antibody

Catalog #: AF1065
14 Citations Datasheet
Catalog # Availability Size / Price Qty
AF1065
AF1065-SP
Detection of Human Adiponectin/Acrp30 by Western Blot.
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Product Details
Citations (14)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Adiponectin/Acrp30 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Adiponectin/Acrp30 in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross‑reactivity with recombinant mouse Adiponectin is observed and less than 15% cross-reactivity with recombinant rat Adiponectin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Adiponectin/Acrp30
Glu19-Asn244
Accession # Q15848
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Adiponectin/Acrp30 antibody by Western Blot. View Larger

Detection of Human Adiponectin/Acrp30 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Adiponectin/Acrp30 at approximately 30-32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Adiponectin/Acrp30 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Adiponectin/Acrp30 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Adiponectin/Acrp30 at approximately 41 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1065) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature human Adiponectin consists of a 60 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-9). Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into > 300 kDa high molecular weight (HMW) oligomers (10-12). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (13). The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities (12, 14).

References
  1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
  2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
  3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
  4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
  5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
  6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
  7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
  8. Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286.
  9. Waki, H. et al. (2005) Endocrinology 146:790.
  10. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
  11. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
  12. Wang, Y. et al. (2008) Biochem. J. 409:623.
  13. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
  14. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.
Entrez Gene IDs
9370 (Human); 11450 (Mouse); 246253 (Rat)
Alternate Names
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein

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Citations for Human Adiponectin/Acrp30 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Plasma diacylglycerol composition is a biomarker of metabolic syndrome onset in rhesus monkeys[S]
    Authors: Michael A. Polewski, Maggie S. Burhans, Minghui Zhao, Ricki J. Colman, Dhanansayan Shanmuganayagam, Mary J. Lindstrom et al.
    Journal of Lipid Research
  2. Chitosan oligosaccharide improves the therapeutic efficacy of sitagliptin for the therapy of Chinese elderly patients with type 2 diabetes mellitus
    Authors: Lijie Zhao, Tingli Sun, Lina Wang
    Therapeutics and Clinical Risk Management
  3. Isthmin-1 attenuates allergic Asthma by stimulating adiponectin expression and alveolar macrophage efferocytosis in mice
    Authors: Tee, JH;Vijayakumar, U;Shanmugasundaram, M;Lam, TYW;Liao, W;Yang, Y;Wong, WSF;Ge, R;
    Respiratory research
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  4. Sensory nerves regulate mesenchymal stromal cell lineage commitment by tuning sympathetic tones
    Authors: B Hu, X Lv, H Chen, P Xue, B Gao, X Wang, G Zhen, JL Crane, D Pan, S Liu, S Ni, P Wu, W Su, X Liu, Z Ling, M Yang, R Deng, Y Li, L Wang, Y Zhang, M Wan, Z Shao, H Chen, W Yuan, X Cao
    J. Clin. Invest., 2020-07-01;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Effects of X-chromosome Tenomodulin Genetic Variants on Obesity in a Children's Cohort and Implications of the Gene in Adipocyte Metabolism
    Authors: FJ Ruiz-Ojeda, A Anguita-Ru, AI Rupérez, C Gomez-Llor, J Olza, R Vázquez-Co, M Gil-Campos, G Bueno, R Leis, R Cañete, LA Moreno, A Gil, CM Aguilera
    Sci Rep, 2019-03-08;9(1):3979.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity
    Authors: K Tang, C Zhang, Y Zhang, Y Zhang, R Zhuang, B Jin, Y Ma
    J Immunol Res, 2017-03-23;2017(0):6468097.
    Species: Human
    Sample Types: Plasma
    Applications: Western Blot
  7. Impact of 3-Amino-1,2,4-Triazole (3-AT)-Derived Increase in Hydrogen Peroxide Levels on Inflammation and Metabolism in Human Differentiated Adipocytes
    Authors: FJ Ruiz-Ojeda, C Gomez-Llor, CM Aguilera, A Gil, AI Rupérez
    PLoS ONE, 2016-03-29;11(3):e0152550.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Heavy Metal Ion Regulation of Gene Expression: MECHANISMS BY WHICH LEAD INHIBITS OSTEOBLASTIC BONE-FORMING ACTIVITY THROUGH MODULATION OF THE Wnt/beta-CATENIN SIGNALING PATHWAY.
    Authors: Beier E, Sheu T, Dang D, Holz J, Ubayawardena R, Babij P, Puzas J
    J Biol Chem, 2015-05-14;290(29):18216-26.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Urinary adiponectin excretion: a novel marker for vascular damage in type 2 diabetes.
    Authors: von Eynatten M, Liu D, Hock C, Oikonomou D, Baumann M, Allolio B, Korosoglou G, Morcos M, Campean V, Amann K, Lutz J, Heemann U, Nawroth PP, Bierhaus A, Humpert PM
    Diabetes, 2009-06-09;58(9):2093-9.
    Species: Human
    Sample Types: Urine
    Applications: Western Blot
  10. Adiponectin and functional adiponectin receptor 1 are expressed by airway epithelial cells in chronic obstructive pulmonary disease.
    Authors: Miller M, Cho JY, Pham A, Ramsdell J, Broide DH
    J. Immunol., 2009-01-01;182(1):684-91.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Improved ELISA for selective measurement of adiponectin multimers and identification of adiponectin in human cerebrospinal fluid.
    Authors: Ebinuma H, Miida T, Yamauchi T, Hada Y, Hara K, Kubota N, Kadowaki T
    Clin. Chem., 2007-06-28;53(8):1541-4.
    Species: Human
    Sample Types: CSF
    Applications: Native SDS-PAGE, Western Blot
  12. Decreased high-molecular-weight adiponectin in gestational diabetes: implications for the pathophysiology of Type 2 diabetes.
    Authors: Retnakaran R, Connelly PW, Maguire G, Sermer M, Zinman B, Hanley AJ
    Diabet. Med., 2007-03-01;24(3):245-52.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot
  13. Tumor necrosis factor alpha and adiponectin in bone marrow interstitial fluid from patients with acute myeloid leukemia inhibit normal hematopoiesis.
    Authors: Iversen PO, Wiig H
    Clin. Cancer Res., 2005-10-01;11(19):6793-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Native adiponectin in serum binds to mammalian cells expressing T-cadherin, but not AdipoRs or calreticulin
    Authors: Shunbun Kita, Shiro Fukuda, Norikazu Maeda, Iichiro Shimomura
    eLife

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