Human Adiponectin/Acrp30 Antibody

Catalog #: MAB10652
2 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
MAB10652
MAB10652-SP

Detection of Human Adiponectin/Acrp30 by Western Blot.

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All Adiponectin/Acrp30 Antibodies

Product Details

Citations (2)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Adiponectin/Acrp30 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Adiponectin/Acrp30 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Acrp30 (aa 101-244), recombinant mouse (rm) Acrp30 (aa 111-247), rmAcrp30 (HWM), recombinant rat Acrp30 (aa 1-244), rhC1qTNF1, rmCORS26/C1qTNF3, rhC1qTNF4, rhC1qTNF5, rhC1qTNF9, or rhC1qL2 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 553517

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Adiponectin/Acrp30
Glu19-Asn244
Accession # Q15848

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Adiponectin/Acrp30 by Western Blot. Western blot shows lysates of human adipose tissue. PVDF Membrane was probed with 2 µg/mL of Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10652) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Adiponectin/Acrp30 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in non-metabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1‑3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin 13/T-Cadherin, as well as with several growth factors (4‑7). Mature human Adiponectin consists of a 60 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8, 9). Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into > 300 kDa high molecular weight (HMW) oligomers (10‑12). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (13). The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities (12, 14).

References

Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
Yamauchi, T. et al. (2007) Nat. Med. 13:332.
Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286.
Waki, H. et al. (2005) Endocrinology 146:790.
Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
Wang, Y. et al. (2008) Biochem. J. 409:623.
Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.

Entrez Gene IDs

9370 (Human); 11450 (Mouse); 246253 (Rat)

Alternate Names

ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human Adiponectin/Acrp30 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Potential Roles of Adiponectin Isoforms in Human Obesity with Delayed Wound Healing
Authors: J Ryu, CA Loza, H Xu, M Zhou, JT Hadley, J Wu, H You, H Wang, J Yang, J Bai, F Liu, C Bialowas, LQ Dong
Cells, 2019-09-24;8(10):.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
Functional effects of adiponectin on endothelial progenitor cells.
Authors: Lavoie V, Kernaleguen AE, Charron G, Farhat N, Cossette M, Mamarbachi AM, Allen BG, Rheaume E, Tardif JC
Obesity (Silver Spring), 2010-09-02;19(4):722-8.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot