Human ADAM8 Ectodomain Antibody

Catalog #: AF1031
10 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
AF1031
AF1031-SP

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Citations (10)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAM8 Ectodomain Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAM8 Ectodomain in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse ADAM8 is observed and less than 1% cross-reactivity with recombinant human (rh) ADAM12, rhADAM19, and rhADAM33 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ADAM8 Ectodomain
Glu158-Ser653
Accession # P78325

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ADAM8 aa 158-497 (Catalog # 1031-AD)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human ADAM8 aa 158-497 (Catalog # 1031-AD), see our available Western blot detection antibodies

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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ADAM8 in Human PBMCs. ADAM8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human ADAM8 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1031) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM8

ADAM8, also known as cell surface antigen MS2 or CD156a, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). ADAM8 can cleave a variety of substrates and has been shown as a sheddase for the low affinity IgE receptor CD23 and the neural recognition molecule CHL1 (3, 4). Expression and regulation studies suggest that ADAM8 is a novel osteoclast stimulating factor and may play a role in asthma (5, 6). The 824 amino acid precursor of human ADAM8 consists of a signal peptide (residues 1 to 16), a pro peptide (residues 17 to 199), a metaloprotease domain (residues 200 to 400), a disintegrin-like domain (residues 408 to 494), a cysteine-rich region (residues 497 to 613), an EGF-like domain (residues 614 to 640), a transmembrane region (residues 656 to 676) and a cytoplasmic domain (residues 677 to 824).

References

Yoshiyama, K. et al. (1997) Genomics 41:56.
Moss, M.L. and J.W. Bartsch (2004) Biochemistry 43:7227.
Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469.
Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
Choi, S.J. et al. (2001) J. Bone Miner. Res. 16:814.
King, N.E. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:257.

Long Name

A Disintegrin and Metalloprotease-like Domain 8

Entrez Gene IDs

101 (Human)

Alternate Names

a disintegrin and metalloproteinase domain 8; ADAM 8; ADAM metallopeptidase domain 8; ADAM8; CD156a antigen; CD156a; CD156human leukocyte differentiation antigen; Cell surface antigen MS2; EC 3.4.24; EC 3.4.24.-; MGC134985; MS2; MS2disintegrin and metalloproteinase domain-containing protein 8

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human ADAM8 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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The metalloproteinase ADAM8 promotes leukocyte recruitment in vitro and in acute lung inflammation
Authors: Daniela Dreymueller, Jessica Pruessmeyer, Julian Schumacher, Sandra Fellendorf, Franz Martin Hess, Anke Seifert et al.
American Journal of Physiology-Lung Cellular and Molecular Physiology
Fibronectin Fragments and the Cleaving Enzyme ADAM-8 in the Degenerative Human Intervertebral Disc
Authors: Nancy Ruel, Dessislava Z. Markova, Sherrill L. Adams, Carla Scanzello, Gabriella Cs-Szabo, David Gerard et al.
Spine (Phila Pa 1976)
A Novel Class of Human ADAM8 Inhibitory Antibodies for Treatment of Triple-Negative Breast Cancer
Authors: Mineva, ND;Pianetti, S;Das, SG;Srinivasan, S;Billiald, NM;Sonenshein, GE;
Pharmaceutics
Species: N/A
Sample Types: Antibody
Applications: Flow Cytometry
RNA Aptamer Targeting of Adam8 in Cancer Growth and Metastasis
Authors: Mi, Z;Kuo, MC;Kuo, PC;
Cancers
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
ADAM8 signaling drives neutrophil migration and ARDS severity
Authors: C Conrad, D Yildiz, SJ Cleary, A Margraf, L Cook, U Schlomann, B Panaretou, JL Bowser, H Karmouty-Q, J Li, NK Berg, SC Martin, A Aljohmani, SF Moussavi-H, KM Wang, JJ Tian, M Magnen, C Valet, L Qiu, JP Singer, HK Eltzschig, CAPSys Stu, W Bertrams, S Herold, N Suttorp, B Schmeck, ZT Ball, A Zarbock, MR Looney, JW Bartsch
JCI Insight, 2022-02-08;7(3):.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, ICC, IHC
Cohort Analysis of ADAM8 Expression in the PDAC Tumor Stroma
Authors: C Jaworek, Y Verel-Yilm, S Driesch, S Ostgathe, L Cook, S Wagner, DK Bartsch, EP Slater, JW Bartsch
Journal of personalized medicine, 2021-02-10;11(2):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Whole body and hematopoietic ADAM8 deficiency does not influence advanced atherosclerotic lesion development, despite its association with human plaque progression
Authors: K Theodorou, EPC van der Vo, MJ Gijbels, IMJ Wolfs, M Jeurissen, TL Theelen, JC Sluimer, E Wijnands, JP Cleutjens, Y Li, Y Jansen, C Weber, A Ludwig, JF Bentzon, JW Bartsch, EAL Biessen, MMPC Donners
Sci Rep, 2017-09-15;7(1):11670.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
ADAM8 as a drug target in pancreatic cancer.
Authors: Schlomann U, Koller G, Conrad C, Ferdous T, Golfi P, Garcia A, Hofling S, Parsons M, Costa P, Soper R, Bossard M, Hagemann T, Roshani R, Sewald N, Ketchem R, Moss M, Rasmussen F, Miller M, Lauffenburger D, Tuveson D, Nimsky C, Bartsch J
Nat Commun, 2015-01-28;6(0):6175.
Species: Human
Sample Types: Whole Cells
Applications: Immunoprecipitation, Native SDS-PAGE
Expression profiles and clinical correlations of degradome components in the tumor microenvironment of head and neck squamous cell carcinoma.
Authors: Stokes A, Joutsa J, Ala-Aho R, Pitchers M, Pennington CJ, Martin C, Premachandra DJ, Okada Y, Peltonen J, Grenman R, James HA, Edwards DR, Kahari VM
Clin. Cancer Res., 2010-03-21;16(0):2022.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
ADAM8 affects glioblastoma progression by regulating osteopontin-mediated angiogenesis
Authors: Yu Li, Songbo Guo, Kai Zhao, Catharina Conrad, Caroline Driescher, Vanessa Rothbart et al.
Biological Chemistry