Human Acetylated p53 (K382) Antibody

Catalog #: MAB13552 Datasheet

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB13552-100
MAB13552-SP

Detection of Human Acetylated p53 (K382) by Western Blot.

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Human Acetylated p53 (K382) Antibody Summary

Species Reactivity

Human

Specificity

Detects human acetylated p53 (K382) in Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2589A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Synthetic peptide containing acetylated human p53 (K382)
Accession # P04637

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

4 µg/mL

HeLa human cervical epithelial carcinoma cell line treated with doxorubicin and trichostatin A (TSA)

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded sections of lung cancer tissue

Immunocytochemistry

3-25 µg/mL

Immersion fixed HeLa human cervical epithelial carcinoma cell line treated with doxorubicin and trichostatin A (TSA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Acetylated p53 (K382) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with doxorubicin and trichostatin A (TSA). PVDF membrane was probed with 4 µg/mL of Rabbit Anti-Human Acetylated p53 (K382) Monoclonal Antibody (Catalog # MAB13552) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for acetylated p53 (K382) at approximately 53 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry

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Acetylated p53 (K382) in Lung Cancer. Acetylated p53 (K382) was detected in immersion fixed paraffin-embedded sections of lung cancer tissue using Rabbit Anti-Human Acetylated p53 (K382) Monoclonal Antibody (Catalog # MAB13552) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry

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Acetylated p53 (K382) in HeLa Human Cell Line. Acetylated p53 (K382) was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with trichostatin A (TSA) and doxorubicin (left panel; positive staining) and untreated HeLa cell line (right panel; negative control) using Rabbit Anti-Human Acetylated p53 (K382) Monoclonal Antibody (Catalog # MAB13552) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p53

The p53 tumor suppressor protein acts to enforce cell cycle checkpoints or signal apoptosis in cells that have incurred genotoxic damage. The ATM or ATR kinases can phosphorylate p53 at serine 15 (S15), which leads to cell cycle arrest. Serine 15 phosphorylation leads to p53 stabilization and enhances transactivation of p53 target genes.

Entrez Gene IDs

7157 (Human); 22059 (Mouse); 24842 (Rat)

Alternate Names

Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53