Goat IgG NorthernLights™ NL557-conjugated Antibody

Catalog #: NL001
37 Citations 2 Reviews Datasheet

NorthernLights 557 Fluorochrome-labeled Antibody, X Absorbed

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NL001

Detection of Goat IgG Primary Antibody by Immunocytochemistry.

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Citations (37)

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Reviews (2)

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Goat IgG NorthernLights™ NL557-conjugated Antibody Summary

Species Reactivity

Goat

Specificity

Goat IgG (H+L)

Source

Polyclonal Donkey IgG

Purification

Antigen Affinity-purified

Immunogen

Goat IgG

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.

Label

NL557 (Excitation= 557 nm, Emission= 574 nm)

Applications

Recommended Concentration

Sample

Immunocytochemistry

Use at a 1:200 dilution

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of Goat IgG Primary Antibody by Immunocytochemistry. Detection of human EGFR by incubating cells with 5 μg/mL of Goat anti-Human EGFR polyclonal antibody (AF231) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A431 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control). Detection of human EGFR by incubating cells with 5 ug/mL of Goat anti-Human EGFR polyclonal antibody (AF231) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A431 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

Product Datasheet

Citations for Goat IgG NorthernLights™ NL557-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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Influence of glucose and oxygen tension on the trophectoderm and the inner cell mass of in vitro produced bovine embryos
Authors: Berling, FP;Mendes, CM;Goissis, MD;
Theriogenology
Species: Bovine
Sample Types: Embryo
Applications: Immunohistochemistry
Nonclassical monocytes potentiate anti-tumoral CD8+ T cell responses in the lungs
Authors: Lindsey E. Padgett, Paola M. Marcovecchio, Claire E. Olingy, Daniel J. Araujo, Kathleen Steel, Huy Q. Dinh et al.
Frontiers in Immunology
Generation of a human iPSC line (CIBi014-A) from a patient with Parkinson's disease carrying a novel heterozygotic PARK8 (LRRK2) mutation
Authors: L Li, X Si, J Yang, M Lei, H Liu, J Ruan, H Fu, W Li, H Yang, X Lei, H Sang
Stem Cell Research, 2022-12-09;66(0):102995.
Species: Human
Sample Types: Whole Cells
Applications: ICC
KDM6A Loss Recruits Tumor-Associated Neutrophils and Promotes Neutrophil Extracellular Trap Formation in Pancreatic Cancer
Authors: J Yang, L Jin, HS Kim, F Tian, Z Yi, K Bedi, M Ljungman, M Pasca di M, H Crawford, J Shi
Cancer Research, 2022-11-15;0(0):OF1-OF14.
Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)
Authors: H Lin, SQ Ye, ZH Xu, JS Penaloza, M Aljuhani, T Vetter, MT Zhao, KL McBride
Stem Cell Research, 2022-10-31;65(0):102958.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Production of a human iPSC line from an early-onset Parkinson's disease patient with a novel CHCHD2 gene truncated mutation
Authors: Z Jiang, XJ Gu, WM Su, QQ Duan, JY Lin, B Cao, HF Shang, YP Chen
Stem Cell Research, 2022-08-04;64(0):102881.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC
Generation of a RRAGA knockout human iPSC line GIBHi002-A-5 using CRISPR/Cas9 technology
Authors: Y Sun, J Fu, J Yang, J Zhao, J Rong
Oncogene, 2022-07-11;63(0):102859.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC
Generation of a human iPSC line CIBi011-A from amniocytes of a healthy fetus
Authors: Y Shi, S Wu, B Yang, B Yu, Y Yan, J Yang, M Chu, X Hu
Stem Cell Research, 2022-05-04;62(0):102801.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Generation of a human induced pluripotent stem cell line FMUPDCi001-A from a patient with mental retardation, autosomal recessive 36 (MRT36) carrying the variants c.219dupA and c.587Cï¿½>ï¿½T in ADAT3
Authors: X Hu, S Xiong, X Zhou, L Sun
Stem Cell Research, 2022-04-04;61(0):102777.
Species: Human
Sample Types: Spheroid
Applications: ICC
CD200S-positive granulated lymphoid cells in endometrium appear to be CD56-positive uterine NK cells
Authors: DA Clark, A Reihani, JL Arredondo, K Ask, WG Foster
Journal of reproductive immunology, 2022-01-14;150(0):103477.
Species: Human
Sample Types: Whole Cells
Applications: IHC

Inflammation and enhanced atherogenesis in the carotid artery with altered blood flow in an atherosclerosis-resistant mouse strain
Authors: J Zhao, C Huangfu, Z Chang, W Zhou, AT Grainger, Z Liu, W Shi
Physiological Reports, 2021-06-01;9(11):e14829.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Generation of a human induced pluripotent stem cell line (SMUSHi001-A) from a patient with 46, XX male sex reversal syndrome carrying the SRY gene
Authors: R Yan, S Wu, W Wang, M Chu, P Liu
Stem Cell Research, 2021-05-24;54(0):102397.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Generation of a human iPSC line QDMHi001-A from a patient with Marfan syndrome carrying a heterozygous c.6772ï¿½Tï¿½>ï¿½C variant in FBN1
Authors: S Wu, Z Zhang, L Wang, J Yu
Stem Cell Research, 2021-05-16;54(0):102390.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Generation of a human iPSC line CIBi009-A from a patient with familial hypercholesterolemia carrying variants of LDLR c.T1241G and APOB c.G1618T
Authors: W Ge, Y Song, M Chu, Y Liu, B Yang, K Wang, B Yu, C Song, Y Wang, J Yang
Stem Cell Research, 2021-04-20;53(0):102347.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC

FSTL1 promotes growth and metastasis in gastric cancer by activating AKT related pathway and predicts poor survival
Authors: M Wu, Y Ding, N Wu, J Jiang, Y Huang, F Zhang, H Wang, Q Zhou, Y Yang, W Zhuo, L Teng
American journal of cancer research, 2021-03-01;11(3):712-728.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Generation of a human iPSC line GIBHi002-A-2 with a dual-reporter for NKX2-5 using TALENs
Authors: M Zhou, R Wei, Y Jiang, J Fu, Y Liu, B Yang, B Yu, Y Lin, X Ran, WH Lai, M Chu, Y Hu, J Yang, HF Tse
Stem Cell Research, 2020-12-10;50(0):102120.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Generation of a human induced pluripotent stem cell line (SBWCHi001-A) from a patient with NEDSDV carrying a pathogenic mutation in CTNNB1 gene
Authors: R Yan, P Liu, F Li, M Chu, J Lei, F Wang, L Luo, X Xu
Stem Cell Res, 2020-11-19;49(0):102091.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Generation of a human iPSC line CIBi008-A from amniotic fluid-derived cells of a fetus with &beta-thalassemia carrying variants of -28Aï¿½>ï¿½G and IVS-II-654Cï¿½>ï¿½T in HBB
Authors: S Wu, H Wei, M Chu, Z Weng
Stem Cell Res, 2020-10-29;49(0):102074.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Colon adenocarcinoma-derived cells that express induced-pluripotent stem cell markers possess stem cell function
Authors: MJ Munro, L Peng, SK Wickremese, ST Tan
PLoS ONE, 2020-05-19;15(5):e0232934.
Species: Human
Sample Types: Whole Cells
Applications: ICC

GPR30 receptor promotes preoperative anxiety-induced postoperative hyperalgesia by up-regulating GABAA-&alpha4&beta1&delta subunits in periaqueductal gray in female rats
Authors: M Jiang, Y Sun, Y Lei, F Hu, Z Xia, Y Liu, Z Ma, X Gu
BMC Anesthesiol, 2020-04-22;20(1):93.
Species: Rats
Sample Types: Tissue
Applications: IHC

Effects of thrombospondin-4 on pro-inflammatory phenotype differentiation and apoptosis in macrophages
Authors: Rahman MT, Muppala S, Wu J et al.
Cell Death Dis

Generation of a knock-in MAP2-tdTomato reporter human embryonic stem cell line with inducible expression of NEUROG2/1 (NYGCe001-A)
Authors: C Lu, NE Sanjana
Stem Cell Res, 2019-11-01;41(0):101643.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Atherogenesis in the Carotid Artery with and without Interrupted Blood Flow of Two Hyperlipidemic Mouse Strains
Authors: Jian Zhao, Chaoji Huangfu, Zhihui Chang, Andrew T. Grainger, Zhaoyu Liu, Weibin Shi
Journal of Vascular Research

Enhanced Tropism of Species B1 Adenoviral-Based Vectors for Primary Human Airway Epithelial Cells
Authors: Ni Li, Ashley L. Cooney, Wenli Zhang, Anja Ehrhardt, Patrick L. Sinn
Molecular Therapy - Methods & Clinical Development

Targeting Tumor Vascular CD99 Inhibits Tumor Growth
Authors: Elisabeth J. M. Huijbers, Inge M. van der Werf, Lisette D. Faber, Lena D. Sialino, Pia van der Laan, Hanna A. Holland et al.
Frontiers in Immunology

Understanding Hematopoietic Stem Cell Development through Functional Correlation of Their Proliferative Status with the Intra-aortic Cluster Architecture
Authors: A Batsivari, S Rybtsov, C Souilhol, A Binagui-Ca, D Hills, S Zhao, P Travers, A Medvinsky
Stem Cell Reports, 2017-05-04;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Plasma cathepsin D correlates with histological classifications of fatty liver disease in adults and responds to intervention
Sci Rep, 2016-12-06;6(0):38278.
Species: Human
Sample Types: Whole Cells
Applications: Secondary Antibody

Collagen-induced arthritis and imiquimod-induced psoriasis develop independently of interleukin-33
Authors: Sara Khaleghparast Athari
Arthritis Res Ther, 2016-06-18;18(1):143.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Interleukin-33 from Monocytes Recruited to the Lung Contributes to House Dust Mite-Induced Airway Inflammation in a Mouse Model
Authors: Hiroki Tashiro
PLoS ONE, 2016-06-16;11(6):e0157571.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

A promyelocytic leukemia protein-thrombospondin 2 axis and the risk of relapse in neuroblastoma
Authors: M Dvorkina, V Nieddu, S Chakelam, A Pezzolo, S Cantilena, AP Leite, O Chayka, T Regad, A Pistorio, AR Sementa, A Virasami, J Barton, X Montano, T Lechertier, N Brindle, D Morgenster, M LE Bras, A Burns, N Saunders, K Hodivala-D, L Bagella, H de The
Clin Cancer Res, 2016-04-13;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

TLR4 Expression Is Associated with Left Ventricular Dysfunction in Patients Undergoing Coronary Artery Bypass Surgery.
Authors: Avlas O, Bragg A, Fuks A, Nicholson J, Farkash A, Porat E, Aravot D, Levy-Drummer R, Cohen C, Shainberg A, Arad M, Hochhauser E
PLoS ONE, 2015-06-01;10(6):e0120175.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Human umbilical cord and dental pulp-derived mesenchymal stem cells: Biological characteristics and potential roles in vitro and in vivo
Authors: XIAOYAN CUI, LEI CHEN, TING XUE, JING YU, JIE LIU, YAZHONG JI et al.
Molecular Medicine Reports

Influence of the CXCL1 rs4074 A allele on alcohol induced cirrhosis and HCC in patients of European descent.
Authors: Nischalke H, Berger C, Lutz P, Langhans B, Wolter F, Eisenhardt M, Kramer B, Kokordelis P, Glassner A, Muller T, Rosendahl J, Fischer J, Berg T, Grunhage F, Leifeld L, Soyka M, Nattermann J, Sauerbruch T, Stickel F, Spengler U
PLoS ONE, 2013-11-18;8(11):e80848.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Functional Properties of Rare Missense Variants of Human CDH13 Found in Adult Attention Deficit/Hyperactivity Disorder (ADHD) Patients
Authors: Thegna Mavroconstanti, Stefan Johansson, Ingeborg Winge, Per M. Knappskog, Jan Haavik
PLoS ONE

CD163 and IgG codefend against cytotoxic hemoglobin via autocrine and paracrine mechanisms.
Authors: Subramanian K, Du R, Tan N, Ho B, Ding J
J Immunol, 2013-04-15;190(10):5267-78.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Suppression of antitumor immunity by stromal cells expressing fibroblast activation protein-alpha.
Authors: Kraman M, Bambrough P, Arnold J, Roberts E, Magiera L, Jones J, Gopinathan A, Tuveson D, Fearon D
Science, 2010-11-05;330(6005):827-30.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Toll-like receptor 2 mediates mesenchymal stem cell-associated myocardial recovery and VEGF production following acute ischemia-reperfusion injury.
Authors: Abarbanell AM, Wang Y, Herrmann JL
Am. J. Physiol. Heart Circ. Physiol., 2010-02-19;298(5):H1529-36.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

FAQs

Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
- All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.

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