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Goat IgG Horseradish Peroxidase-conjugated Antibody

Catalog #: HAF019
40 Citations Datasheet
Catalog # Availability Size / Price Qty
HAF019
Detection of Goat IgG Primary Antibody by Western Blot.
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Citations (40)
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Goat IgG Horseradish Peroxidase-conjugated Antibody Summary

Species Reactivity
Goat
Specificity
Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with mouse IgG, rabbit IgG and human IgG is observed.
Source
Polyclonal Chicken IgY
Purification
Antigen Affinity-purified from egg yolks
Immunogen
Goat IgG
Formulation
Supplied as a 0.2 μm filtered solution in Sodium Phosphate and Proclin 300.
Label
HRP(Excitation= nm, Emission= nm)

Applications

Recommended Concentration
Sample
Western Blot
Use at a 1:1000 dilution.
 
Immunohistochemistry
Use at a 1:20dilution 
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Goat IgG Primary Antibody by Western Blot. View Larger

Detection of Goat IgG Primary Antibody by Western Blot. Western blot shows detection of Goat Anti-Human/Mouse/Rat Carboxylesterase 1/CES1 Polyclonal Antibody (AF4920) using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry View Larger

Detection of Vimentin in Human Kidney Detection of Vimentin in human kidney using 5 ug/mL of Goat anti-human Vimentin primary antibody (AF2105) followed by 30 min incubation with Donkey Anti-Goat HRP conjugated secondary antibodies (Catalog# HAF019) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Goat IgG Horseradish Peroxidase-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

40 Citations: Showing 1 - 10
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  1. Combined LXR and RXR Agonist Therapy Increases ABCA1 Protein Expression and Enhances ApoAI-Mediated Cholesterol Efflux in Cultured Endothelial Cells
    Authors: Huang K, Jo H, Echesabal-Chen J, Stamatikos A
    Metabolites
  2. Hepatocyte nuclear factors as possible C-reactive protein transcriptional inducer in the liver and white adipose tissue of rats with experimental chronic renal failure
    Authors: Elzbieta Sucajtys-Szulc, Alicja Debska-Slizien, Boleslaw Rutkowski, Ryszard Milczarek, Iwona Pelikant-Malecka, Tomasz Sledzinski et al.
    Molecular and Cellular Biochemistry
  3. Compromised NLRP3 and AIM2 inflammasome function in autoimmune NZB/W F1 mouse macrophages
    Authors: SJ Thygesen, KE Takizawa, AAB Robertson, DP Sester, KJ Stacey
    Immunol. Cell Biol., 2018-08-19;0(0):.
  4. Mitochondrial oxidative stress-induced transcript variants of ATF3 mediate lipotoxic brain microvascular injury
    Authors: Nyunt T, Britton M, Wanichthanarak K et al.
    Free Radic. Biol. Med.
  5. Dysregulation of the renin-angiotensin system during lung ischemia-reperfusion injury
    Authors: K. Kehoe, J. F. Gielis, G. Vliegen, R. Van Elzen, R. Verkerk, E. Driessens et al.
    Experimental Lung Research
  6. Trop2 inhibition suppresses the proliferation and invasion of laryngeal carcinoma cells via the extracellular signal-regulated kinase/mitogen-activated protein kinase pathway
    Authors: XU-DONG WANG, QIANG WANG, XIAO-LIN CHEN, JIAN-FEI HUANG, YONG YIN, PENG DA et al.
    Molecular Medicine Reports
  7. Zoledronic acid and thymosin alpha 1 elicit antitumor immunity against prostate cancer by enhancing tumor inflammation and cytotoxic T cells
    Authors: Sheng Wang, Maohua Huang, Minfeng Chen, Zhiting Sun, Yubo Jiao, Geni Ye et al.
    Journal for ImmunoTherapy of Cancer
  8. Fiber‐Optic Theranostics (FOT): Interstitial Fiber‐Optic Needles for Cancer Sensing and Therapy
    Authors: Yang Ran, Zhiyuan Xu, Minfeng Chen, Wei Wang, Yang Wu, Jiexuan Cai et al.
    Advanced Science
  9. Inflammatory responses induced by Helicobacter pylori on the carcinogenesis of gastric epithelial GES‑1 cells
    Authors: Jianjun Wang, Yongliang Yao, Qinghui Zhang, Shasha Li, Lijun Tang
    International Journal of Oncology
  10. Genetic risk factors for COVID-19 and influenza are largely distinct
    Authors: Kosmicki, JA;Marcketta, A;Sharma, D;Di Gioia, SA;Batista, S;Yang, XM;Tzoneva, G;Martinez, H;Sidore, C;Kessler, MD;Horowitz, JE;Roberts, GHL;Justice, AE;Banerjee, N;Coignet, MV;Leader, JB;Park, DS;Lanche, R;Maxwell, E;Knight, SC;Bai, X;Guturu, H;Baltzell, A;Girshick, AR;McCurdy, SR;Partha, R;Mansfield, AJ;Turissini, DA;Zhang, M;Mbatchou, J;Watanabe, K;Verma, A;Sirugo, G;Colorado Center for Precision Medicine, ;Mayo Clinic Project Generation, ;Regeneron Genetics Center, ;University of California Los Angeles ATLAS Collaboration, ;Ritchie, MD;Salerno, WJ;Shuldiner, AR;Rader, DJ;Mirshahi, T;Marchini, J;Overton, JD;Carey, DJ;Habegger, L;Reid, JG;Economides, A;Kyratsous, C;Karalis, K;Baum, A;Cantor, MN;Rand, KA;Hong, EL;Ball, CA;Siminovitch, K;Baras, A;Abecasis, GR;Ferreira, MAR;
    Nature genetics
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Endothelial cell Piezo1 promotes vascular smooth muscle cell differentiation on large arteries
    Authors: Abello, J;Yin, Y;Zhao, Y;Maurer, J;Lee, J;Bodell, C;Clevenger, AJ;Burton, Z;Goeckel, ME;Lin, M;Grainger, S;Halabi, CM;Raghavan, SA;Sah, R;Stratman, AN;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Molecular basis for human aquaporin inhibition
    Authors: Huang, P;Åbacka, H;Wilson, CJ;Wind, ML;R?tzler, M;Hagström-Andersson, A;Gourdon, P;de Groot, BL;Venskutonyt?, R;Lindkvist-Petersson, K;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Lipocalin 2 regulates mitochondrial phospholipidome remodeling, dynamics, and function in brown adipose tissue in male mice
    Authors: Su, H;Guo, H;Qiu, X;Lin, TY;Qin, C;Celio, G;Yong, P;Senders, M;Han, X;Bernlohr, DA;Chen, X;
    Nature communications
    Species: Mouse
    Sample Types: Lipids
    Applications: Bioassay
  14. Liver-specific in vivo base editing of Angptl3 via AAV delivery efficiently lowers blood lipid levels in mice
    Authors: Zuo, Y;Zhang, C;Zhou, Y;Li, H;Xiao, W;Herzog, RW;Xu, J;Zhang, J;Chen, YE;Han, R;
    Cell & bioscience
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  15. Subfunctionalized expression drives evolutionary retention of ribosomal protein paralogs Rps27 and Rps27l in vertebrates
    Authors: Xu, AF;Molinuevo, R;Fazzari, E;Tom, H;Zhang, Z;Menendez, J;Casey, KM;Ruggero, D;Hinck, L;Pritchard, JK;Barna, M;
    eLife
    Species: Mouse
    Sample Types: Cell Lysates
  16. Hepatocyte Nuclear Factor-1alpha Increases Fibrinogen Gene Expression in Liver and Plasma Fibrinogen Concentration in Rats with Experimental Chronic Renal Failure
    Authors: E Sucajtys-S, A Debska-Sli, B Rutkowski, R Milczarek, M Szolkiewic, J Swierczyns, RT Smolenski
    International Journal of Molecular Sciences, 2023-03-17;24(6):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. Mycobacterium vaccae protects against glucocorticoid resistance resulting from combined physical and psychosocial trauma in mice
    Authors: D Langgartne, M Amoroso, E Kempter, M Kustermann, J Scheurer, CA Lowry, G Strau beta, SO Reber
    Brain, Behavior, and Immunity, 2023-02-01;109(0):221-234.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Hepatocyte Nuclear Factor 1alpha Proinflammatory Effect Linked to the Overexpression of Liver Nuclear Factor-kappaB in Experimental Model of Chronic Kidney Disease
    Authors: E Sucajtys-S, A Debska-Sli, B Rutkowski, M Szolkiewic, J Swierczyns, RT Smolenski
    International Journal of Molecular Sciences, 2022-08-10;23(16):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. Oct4 differentially regulates chromatin opening and enhancer transcription in pluripotent stem cells
    Authors: L Xiong, EA Tolen, J Choi, S Velychko, L Caizzi, T Velychko, K Adachi, CM MacCarthy, M Lidschreib, P Cramer, HR Schöler
    Elife, 2022-05-27;11(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Mechanisms of angiogenic incompetence in Hutchinson-Gilford progeria via downregulation of endothelial NOS
    Authors: YG Gete, LW Koblan, X Mao, M Trappio, B Mahadik, JP Fisher, DR Liu, K Cao
    Aging Cell, 2021-06-04;0(0):e13388.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Donor cell memory confers a metastable state of directly converted cells
    Authors: KP Kim, C Li, D Bunina, HW Jeong, J Ghelman, J Yoon, B Shin, H Park, DW Han, JB Zaugg, J Kim, T Kuhlmann, RH Adams, KM Noh, SA Goldman, HR Schöler
    Cell Stem Cell, 2021-04-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Intermedin Alleviates Renal Ischemia-Reperfusion Injury and Enhances Neovascularization in Wistar Rats
    Authors: Y Wang, Y Mi, J Tian, X Qiao, X Su, J Kang, Z Wu, G Wang, X Zhou, Y Zhou, R Li
    Drug Des Devel Ther, 2020-11-10;14(0):4825-4834.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  23. Reprogramming competence of OCT factors is determined by transactivation domains
    Authors: KP Kim, Y Wu, J Yoon, K Adachi, G Wu, S Velychko, CM MacCarthy, B Shin, A Röpke, MJ Arauzo-Bra, M Stehling, DW Han, Y Gao, J Kim, S Gao, HR Schöler
    Science Advances, 2020-09-02;6(36):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Quantification of Pharmacokinetic Profiles of PD-1/PD-L1 Antibodies by Validated ELISAs
    Authors: S Zalba, AM Contreras-, E Martisova, R Debets, C Smerdou, MJ Garrido
    Pharmaceutics, 2020-06-26;12(6):.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Detection
  25. Sialylation of FGFR1 by ST6Gal?I overexpression contributes to ovarian cancer cell migration and chemoresistance
    Authors: L Ou, X He, N Liu, Y Song, J Li, L Gao, X Huang, Z Deng, X Wang, S Lin
    Mol Med Rep, 2020-01-20;21(3):1449-1460.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. The Role of Autophagy in the Degradation of Misfolded HLA-B27 Heavy Chains
    Authors: F Navid, G Layh-Schmi, KA Sikora, A Cougnoux, RA Colbert
    Arthritis & rheumatology (Hoboken, N.J.), 2018-03-25;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Western Blot
  27. Thrombin-reduced miR-27b attenuates platelet angiogenic activities in vitro via enhancing platelet synthesis of anti-angiogenic thrombospondin-1
    Authors: X Miao, MF Rahman, L Jiang, Y Min, S Tan, H Xie, L Lee, M Wang, RE Malmström, WO Lui, N Li
    J. Thromb. Haemost., 2018-03-15;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Role of CCL20/CCR6 and the ERK signaling pathway in lung adenocarcinoma
    Authors: XP Zhang, ZJ Hu, AH Meng, GC Duan, QT Zhao, J Yang
    Oncol Lett, 2017-10-23;14(6):8183-8189.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  29. Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity
    Authors: K Tang, C Zhang, Y Zhang, Y Zhang, R Zhuang, B Jin, Y Ma
    J Immunol Res, 2017-03-23;2017(0):6468097.
    Species: Human
    Sample Types: Plasma
    Applications: Western Blot
  30. Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels
    Authors: E Casta¤o, L Caviedes, S Hirsch, M Llanos, G I¤iguez, AM Ronco
    PLoS ONE, 2017-01-19;12(1):e0170389.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  31. Deficient NLRP3 and AIM2 Inflammasome Function in Autoimmune NZB Mice.
    Authors: Sester D, Sagulenko V, Thygesen S, Cridland J, Loi Y, Cridland S, Masters S, Genske U, Hornung V, Andoniou C, Sweet M, Degli-Esposti M, Schroder K, Stacey K
    J Immunol, 2015-06-26;195(3):1233-41.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  32. Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
    Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
    Biomark Insights, 2012-07-10;7(0):87-104.
    Species: Mouse
    Sample Types: BALF
    Applications: Western Blot
  33. BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation.
    Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D
    PLoS ONE, 2010-09-24;5(9):e12995.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  34. Influence of coagulation factor x on in vitro and in vivo gene delivery by adenovirus (Ad) 5, Ad35, and chimeric Ad5/Ad35 vectors.
    Authors: Greig JA, Buckley SM, Waddington SN, Parker AL, Bhella D, Pink R, Rahim AA, Morita T, Nicklin SA, McVey JH, Baker AH
    Mol. Ther., 2009-07-14;17(10):1683-91.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  35. Small extracellular vesicle-mediated ITGB6 siRNA delivery downregulates the alpha V beta 6 integrin and inhibits adhesion and migration of recipient prostate cancer cells
    Authors: Shiv Ram Krishn, Vaughn Garcia, Nicole M. Naranjo, Fabio Quaglia, Christopher D. Shields, Maisha A. Harris et al.
    Cancer Biology & Therapy
  36. Single-cell transcriptional and functional analysis of dopaminergic neurons in organoid-like cultures derived from human fetal midbrain
    Authors: Marcella Birtele, Petter Storm, Yogita Sharma, Janko Kajtez, Jenny Nelander Wahlestedt, Edoardo Sozzi et al.
    Development
  37. Controlling tissue patterning by translational regulation of signaling transcripts through the core translation factor eIF3c
    Authors: Fujii K, Zhulyn O, Byeon GW Et al.
    Developmental cell
  38. Secreted Frizzled Related Protein 4 Reduces Fibrosis Scar Size and Ameliorates Cardiac Function After Ischemic Injury
    Authors: Kentaro Matsushima, Takashi Suyama, Chiemi Takenaka, Naoki Nishishita, Keiko Ikeda, Yoshito Ikada et al.
    Tissue Engineering Part A
  39. Up-regulation of liver Pcsk9 gene expression as a possible cause of hypercholesterolemia in experimental chronic renal failure
    Authors: Elzbieta Sucajtys-Szulc, Marek Szolkiewicz, Julian Swierczynski, Boleslaw Rutkowski
    Molecular and Cellular Biochemistry
  40. Neutrophil-derived IL-1B is sufficient for abscess formation in immunity against Staphylococcus aureus in mice.
    Authors: Cho JS, Guo Y, Ramos RI et al.
    PLoS Pathog.

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