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Goat F(ab')2 Anti-mouse IgM-Fluorescein Antibody

Catalog #: F0118
3 Citations Datasheet
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F0118
R&D Systems Antibodies
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Citations (3)
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Goat F(ab')2 Anti-mouse IgM-Fluorescein Antibody Summary

Species Reactivity
Mouse
Specificity
Mouse IgM specific IgG was purified by goat IgG affinity chromatography and then adsorbed to eliminate human cross reactivity. The IgG fraction was digested with pepsin to generate F(ab')2 fragments which have a reduced ability to interact with Fc receptors expressed on a variety of cells. 
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse IgM heavy and light chains
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter molecule, is of mouse origin and is of the IgM class.The recommended concentration is 10 µL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgM

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin M
Alternate Names
AGM1; DKFZp686I15196; DKFZp686I15212; FLJ00385; IgM; immunoglobulin heavy constant mu; MGC104996; MGC52291; MU; VH

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Citations for Goat F(ab')2 Anti-mouse IgM-Fluorescein Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Comparative Study of Human Dental Follicle Cell Sheets and Periodontal Ligament Cell Sheets for Periodontal Tissue Regeneration
    Authors: Shujuan Guo, Weihua Guo, Yi Ding, Jian Gong, Qing Zou, Dan Xie et al.
    Cell Transplantation
  2. Alterations of bovine nucleus pulposus cells with aging
    Authors: Molinos, M;Fiordalisi, MF;Caldeira, J;Almeida, CR;Barbosa, MA;Gonçalves, RM;
    Aging cell
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Characterization of mesenchymal progenitor cells isolated from human bone marrow by negative selection.
    Authors: Modder UI, Roforth MM, Nicks KM, Peterson JM, McCready LK, Monroe DG, Khosla S
    Bone, 2011-12-28;50(3):804-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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