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GMP Human T Cell Media

Catalog #: CCM038-GMP-1L Datasheet
For robust T cell expansion with GMP-grade media in xeno-free conditions
Catalog # Availability Size / Price Qty
CCM038-GMP-1L
CCM038-GMP-1B
GMP Human T Cell Media, Bagged
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GMP Human T Cell Media Summary

GMP Media for the expansion and culture of human T cells under xeno-free conditions.
 

Key Benefits

• Phenol Red-Free formulation in 1 L bottles (CCM038-GMP-1L) or 1 L bags (CCM038-GMP-1B)
• Robust T cell expansion under xeno-free conditions
• Optimized for use with Bio-Techne cytokines and G-Rex® bioreactors
• Compatible with a variety of T cell activation methods
• Supports T cell expansion from PBMCs

GMP Human T Cell Media is formulated for the ex vivo culture of human T lymphocytes for preclinical or clinical ex vivo use. This media is optimized for T cell expansion in a G-Rex bioreactor with R&D Systems GMP cytokines, in a variety of combinations (IL-7 and IL-15, or alternatively, IL-2). It is also compatible with different cell activation methods, including particle, bead-based, or plate-bound. It is highly recommended to supplement this product with human AB serum. The activation reagents, culture vessel, and cytokine growth factor combinations used with this media should be optimized by application or experimental protocol.

The bagged format of GMP T cell media (CCM038-GMP-1B) is designed to integrate seamlessly into a closed system workflow. Use with ProPak GMP cytokines and a G-Rex bioreactor for a derisked T cell manufacturing process.

Kit Contents

• 1 L Bottle of GMP Human T Cell Media (CCM038-GMP-1L), or
• 1 L Bag of GMP Human T Cell Media (CCM038-GMP-1B)

Specifications

Osmolality
280-330
Sterility Testing
Tested following USP <71> sterility guidelines.
Shipping Conditions
The product is shipped at 2-8 °C. Upon receipt, store immediately at the recommended temperature. Protect from light.
Storage
Store the unopened product at 2-8 °C and protected from light. Do not use past the use by date on the Certificate of Analysis.
Species
Human

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Scientific Data

Data T cells grown in a G-Rex® bioreactor show similar confluency when using human AB serum or a xeno-free serum replacement. View Larger

T cells grown in a G-Rex® bioreactor show similar confluency when using Human AB serum or a xeno-free serum replacement. PBMCs from 3 donors were grown with 10 ng/mL of BT-007-GMP and 10 ng/mL of BT-015-GMP in a G-Rex® bioreactor with either a xeno-free serum replacement or 5% human AB serum.

Data Average fold expansion and number of cells are similar across media suppliers. View Larger

T cell culture fold expansion and viability are similar across other media suppliers.

Data PD-1 and Tim-3 exhaustion markers are lower when culturing T cells in GMP T cell media. View Larger

PD-1 and Tim-3 exhaustion markers are lower when culturing T cells in GMP T cell media.

Data Phenotypic analysis of memory T cell markers CCR7 and CD45RA across suppliers. View Larger

Phenotypic analysis of memory T cell markers CCR7 and CD45RA.

Data Fold expansion of transduced and transposed T cells compared to non-edited T cells. View Larger

Fold expansion of transduced and transposed T cells compared to non-edited T cells.

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