Glo Substrate Reagent Pack Summary
INTENDED USE & DESCRIPTION
Glo Substrate Reagent Pack contains 5 vials of Glo Reagent A (stabilized enhanced luminol, Part # 895868, 4 mL/vial) and 5 vials of Glo Reagent B (stabilized hydrogen peroxide, Part # 895869, 8 mL/vial) for use in chemiluminescent immunoassay development.
STORAGE & STABILITY
Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. Use within 15 minutes to 4 hours of mixing.
PROCEDURE
This reagent may be substituted for H2O2/tetramethylbenzidine as a substrate for horseradish peroxidase in ELISAs. Use black microplates for chemiluminescent assays (Catalog # DY991). Mix 1 part Glo Reagent A to 2 parts Glo Reagent B in a plastic container, cap, and protect from light. Add 100 µL of the mixture to each well and incubate at room temperature while protecting from light. Read the microplate on a luminometer within 5-20 minutes.
Specifications
Product Datasheets
Citations for Glo Substrate Reagent Pack
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Pharmacological inhibition of sodium-calcium exchange activates NADPH oxidase and induces infection-independent NETotic cell death
Authors: M Inoue, M Enomoto, M Yoshimura, T Mizowaki
Redox Biology, 2021-04-26;43(0):101983. 2021-04-26
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Functional Evaluation of AMD-Associated Risk Variants of Complement Factor B
Authors: C Pilotti, J Greenwood, SE Moss
Invest. Ophthalmol. Vis. Sci., 2020-05-11;61(5):19. 2020-05-11
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Plasma redox imbalance caused by albumin oxidation promotes lung-predominant NETosis and pulmonary cancer metastasis
Authors: M Inoue, R Nakashima, M Enomoto, Y Koike, X Zhao, K Yip, SH Huang, JN Waldron, M Ikura, FF Liu, SV Bratman
Nat Commun, 2018-11-30;9(1):5116. 2018-11-30
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Leptin Levels Are Higher in Whole Compared to Skim Human Milk, Supporting a Cellular Contribution
Authors: Foteini Kakulas
Nutrients, 2016-11-08;8(11):. 2016-11-08
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Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
Authors: Khankin EV, Mutter WP, Tamez H
PLoS ONE, 2010-02-16;5(2):e9246. 2010-02-16
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First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
Authors: Thadhani R, Powe CE, Tjoa ML
Diabetes Care, 2009-12-10;33(3):664-9. 2009-12-10
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