Gemcitabine hydrochloride

Biological Activity

Gemcitabine hydrochloride is a deoxycytidine analog that inhibits DNA synthesis. Metabolized to form gemcitabine triphosphate (dFdCTP) and gemcitabine diphosphate (dFdCDP). dFdCTD inhibits ribonucleotide reductase causing a reduction in cellular nucleotides. dFdCTP is incorporated in DNA resulting in DNA strand termination. Displays antitumor activity in vitro and in vivo. Inhibits replication of MERS-CoV and SARS-CoV in vitro (EC₅₀ values are 1.216 and 4.957 μM, respectively).

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Critical Roles of Chemoresistant Effector and Regulatory T Cells in Antitumor Immunity after Lymphodepleting Chemotherapy.
   Saida Y, Watanabe S, Tanaka T, Baba J, Sato K, Shoji S, Igarashi N, Kondo R, Okajima M, Koshio J, Ichikawa K, Nozaki K, Ishikawa D, Koya T, Miura S, Tanaka J, Kagamu H, Yoshizawa H, Nakata K, Narita I
   J Immunol, 2015;195(2):726-35.
2. DPEP1 inhibits tumor cell invasiveness, enhances chemosensitivity and predicts clinical outcome in pancreatic ductal adenocarcinoma.
   Zhang G, Schetter A, He P, Funamizu N, Gaedcke J, Ghadimi BM, Ried T, Hassan R, Yfantis HG, Lee DH, Lacy C, Maitra A, Hanna N, Alexander HR, Hussain SP
   PLoS ONE, 2012;7(2):e31507.
3. Gemcitabine: a modulator of intracellular nucleotide and deoxynucleotide metabolism.
   Heinemann et al.
   Semin.Oncol., 1995;22:11
4. Evaluation of the antitumor activity of gemcit. (2',2'-Difluoro-2'-deoxycytidine).
   Hertel et al.
   Cancer Res., 1990;50:4417
5. Preclinical characteristics of gemcit.
   Plunkett et al.
   Anticancer Drugs, 1995;6:7
6. Repurposing of clinically developed drugs for treatment of Middle East Respiratory Syndrome coronavirus infection.
   Dyall et al.
   Antimicrob.Agents Chemother., 2014;58:4885

Product Datasheets