Fluorescein Tyramide
Chemical Name: 3',6'-Dihydroxy-N-(4-hydroxyphenylethyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5(6)-carboxamide
Purity: ≥95%
Biological Activity
Fluorescein Tyramide is a green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.Optical Data for Fluorescein Tyramide
| Emission Color | Green |
|---|---|
| λabs | 494 nm |
| λem | 517 nm |
| Extinction Coefficient (ε) | 70000 M-1cm-1 |
| Closest Laser line | 488 nm |
| Application | Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays.
Bobrow et al.
J.Immunol.Methods, 1989;125:279 -
Enzymatic signal amplification for sensitive detection of intracellular antigens by flow cytometry.
Karkmann et al.
J.Immunol.Methods, 1999;230:113 -
Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size.
Schriml et al.
Biotechniques, 1999;27:608 -
Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for in situ hybridization using CARD amplification.
Hopman et al.
J.Histochem.Cytochem., 1998;46:771 -
Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization.
van Gijlswijk et al.
J.Histochem.Cytochem., 1997;45:375
Product Datasheets
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