Feline IL-10 Antibody

Catalog #: MAB736 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB736
MAB736-SP

Cell Proliferation Induced by IL‑10 and Neutralization by Feline IL‑10 Antibody.

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Feline IL-10 Antibody Summary

Species Reactivity

Feline

Specificity

Detects feline IL-10 in direct ELISAs and Western blots. In direct ELISAs, 40% cross‑reactivity with canine IL‑10 is observed and no cross‑reactivity with IL-10 from human, mouse, rat, Epstein-Barr virus, or human cytomegalovirus is observed.

Source

Monoclonal Mouse IgG₁ Clone # 137706

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant feline IL‑10
Ser19-Ile178
Accession # P55029

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Feline IL‑10 (Catalog # 736-FL)

Neutralization

Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND₅₀) is typically 0.2-0.6 µg/mL in the presence of 2 ng/mL Recombinant Feline IL‑10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑10 and Neutralization by Feline IL‑10 Antibody. Recombinant Feline IL‑10 (Catalog # 736-FL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL‑10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Feline IL‑10 Monoclonal Antibody (Catalog # MAB736). The ND₅₀ is typically 0.2-0.6 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones. The feline homologue of murine IL-10 has been cloned. Feline IL-10 shares 78% amino acid sequence homology with human IL-10 and 73% sequence homology with mouse IL-10. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, downregulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development.

References

Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165.

Long Name

Interleukin 10

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)

Alternate Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF