Feline Fas/TNFRSF6/CD95 Antibody Summary
Ala25-Lys172
Accession # NP_001009314
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Fas/TNFRSF6/ CD95 in Feline PBMCs treated with PMA and Ca2+ionomcyin by Flow Cytometry. Feline peripheral blood mononuclear cells were treated with PMA and Ca2+ionomycin, then stained with Mouse Anti-Feline Fas/ TNFRSF6/CD95 Mono-clonal Antibody (Catalog # MAB2267, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fas/TNFRSF6/CD95
Feline Fas (fibroblast associated; also named CD95 and APO-1) is a 45 kDa type I transmembrane (TM) glycoprotein that is a member of the TNF receptor superfamily (1-3). The family contains about 30 members, and is characterized by the presence of at least one cysteine-rich domain that contains multiple intrachain disulfide bonds. In general, the superfamily is divided into cytoplasmic death domain (DD) containing, and non-DD containing receptors (3). Feline Fas is synthesized as a 314 amino acid (aa) precursor that contains a 24 aa signal sequence, a 148 aa extracellular region, a 16 aa TM segment, and a 126 aa cytoplasmic tail (4). The extracellular region contains four potential N-linked glycosylation sites plus two distinct cysteine-rich domains of approximately 40 aa each; the cytoplasmic tail shows a 45 aa DD. The extracellular region of feline Fas shares 68%, 65%, 53%, and 58% aa sequence identity to porcine, human, mouse, and rat Fas, respectively. There are five alternate splice forms of feline Fas, which vary from 132 aa to 209 aa in length. All utilize exons 1-3 (aa 1-111) and all are missing the transmembrane segment of the full length form (5). Circulating Fas is reported to be both a dimer and trimer at low ng/mL concentrations. The ligand for Fas is FasL, and Fas ligation activates both the MEK cascade and FADD/caspase-8 pathway (7).
- Locksley, R.M. et al. (2001) Cell 104:487.
- Gaur, U. and B.B. Aggarwal (2003) Biochem. Pharmacol. 66:1403.
- Collette, Y. et al. (2003) Trends Immunol. 24:387.
- Mizuno, T. et al. (1998) Vet. Immunol. Immunopathol. 65:161.
- Mizuno, T. et al. (2004) Eur. J. Immunogenet. 31:159.
- Knipping, E. et al. (1995) Blood 85:1562.
- Baker, S.J. and E.P. Reddy (1998) Oncogene 17:3261.
Product Datasheets
Citation for Feline Fas/TNFRSF6/CD95 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Prior Puma Lentivirus Infection Modifies Early Immune Responses and Attenuates Feline Immunodeficiency Virus Infection in Cats
Authors: WS Sprague, RM Troyer, X Zheng, BA Wood, M Macmillian, S Carver, S VandeWoude
Viruses, 2018-04-20;10(4):.
Species: Feline
Sample Types: Whole Cells
Applications: Flow Cytometry
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