Home / Buffers, Chelators and Reagents / ELISpot Blue Color Module (Strep-AP and BCIP-NBT), 5 Plate

ELISpot Blue Color Module (Strep-AP and BCIP-NBT), 5 Plate

Catalog #: SEL002
40 Citations Datasheet
Catalog # Availability Size / Price Qty
SEL002
R&D Systems ELISpot Development Modules
1 Image
Product Details
Citations (40)
FAQs
Supplemental Products
Reviews
Summary Product Datasheets Preparation and Storage

ELISpot Blue Color Module (Strep-AP and BCIP-NBT), 5 Plate Summary

Assay Type
Quantitative Sandwich ELISA Development Module
Format
96-well microplate, sold separately (see Other Reagents Required)
Assay Length
3 hours 35 mins to 4 hours 50 mins**
Sample Type
Whole Cells
Sufficient Materials
For five 96-well microplates
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

PRODUCT SUMMARY
Complete ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. Kits are available for detection and enumeration of a single analyte or two analytes simultaneously. Complete ELISpot kits are ready-to-run and require no assay development or refinement. ELISpot Development Modules contain the basic components required to develop an ELISpot assay. They offer an economical alternative to buying separate antibodies.

ELISpot development modules are an alternative to ELISpot kits. A basic understanding of ELISpot assay development is required for the successful use of these reagents. Each investigator should optimize the coating conditions, the assay sensitivity, the type of enzyme and substrate, as well as the concentrations of the capture and detection antibodies to achieve desired results. The analyte-specific ELISpot Development Module and the ELISpot Blue Color Module contain the necessary components for analyte detection and visualization, respectively. These modules can be used together but are sold separately. Each module contains enough reagents for at least five 96-well microplates.

PRODUCT FEATURES

  • An economical alternative to ELISpot Kits
  • Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
  • Generic development protocols provide direction to start an optimization protocol
  • Customize the assay to your specific needs

 

MODULE CONTENTS

  • Streptavidin-AP Concentrate
  • BCIP/NBT Chromogen

 

OTHER REAGENTS REQUIRED

  • PBS - 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
  • Wash Buffer - 0.05% Tween® 20 in PBS
  • Reagent Diluent - 1% BSA in PBS, pH 7.2 - 7.4, 0.2 µm filtered
  • Deionized H2O

 

Trademarks and registered trademarks are the property of their respective companies.

Product Datasheets

You must select a language.

x
Product Datasheet

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
⚠ WARNING: This product can expose you to chemicals including methanol, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.

Citations for ELISpot Blue Color Module (Strep-AP and BCIP-NBT), 5 Plate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

40 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Multiple sclerosis patient-derived spontaneous B cells have distinct EBV and host gene expression profiles in active disease
    Authors: Soldan, SS;Su, C;Monaco, MC;Yoon, L;Kannan, T;Zankharia, U;Patel, RJ;Dheekollu, J;Vladimirova, O;Dowling, JW;Thebault, S;Brown, N;Clauze, A;Andrada, F;Feder, A;Planet, PJ;Kossenkov, A;Schäffer, DE;Ohayon, J;Auslander, N;Jacobson, S;Lieberman, PM;
    Nature microbiology
    Species: Human
    Sample Types: Whole Cells
  2. A prospective CSFV-PCV2 bivalent vaccine effectively protects against classical swine fever virus and porcine circovirus type 2 dual challenge and prevents horizontal transmission
    Authors: Chen, JY;Wu, CM;Chia, MY;Huang, C;Chien, MS;
    Veterinary research
    Species: N/A
    Sample Types: Antibody
  3. Unstable EBV latency drives inflammation in multiple sclerosis patient derived spontaneous B cells
    Authors: S Soldan, C Su, MC Monaco, N Brown, A Clauze, F Andrada, A Feder, P Planet, A Kossenkov, D Schäffer, J Ohayon, N Auslander, S Jacobson, P Lieberman
    Research square, 2023-02-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
  4. Adjuvant effect of dendritic cells activator Imiquimod in genetic immunization with HIV-1 p55 Gag
    Authors: Alves, JM;Inyushin, M;Tsytsarev, V;Roldan-Kalil, JA;Miranda-Valentin, E;Maldonado-Martinez, G;Ramos-Feliciano, KM;Hunter-Mellado, R;
    Journal of immunological techniques in infectious diseases
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISpot Capture
  5. Evaluation of the Delivery of a Live Attenuated Porcine Reproductive and Respiratory Syndrome Virus as a Unit Solid Dose Injectable Vaccine
    Authors: E Hayhurst, E Rose, M Pedrera, JC Edwards, N Kotynska, D Grainger, Y Sadigh, J Flannery, L Bonnet, R Ritwik, P Dulal, MK Howard, SP Graham
    Vaccines, 2022-10-30;10(11):.
    Species: Porcine
    Sample Types: Whole Cells
  6. Early human B cell signatures of the primary antibody response to mRNA vaccination
    Authors: L Kardava, N Rachmanino, WW Lau, CM Buckner, K Trihemasav, J Blazkova, F Lopes de A, W Wang, X Zhang, Y Wang, CI Chiang, S Narpala, GE McCormack, C Liu, CA Seamon, MC Sneller, S O'Connell, Y Li, AB McDermott, TW Chun, AS Fauci, JS Tsang, S Moir
    Oncogene, 2022-06-27;119(28):e2204607119.
    Species: Human
    Sample Types: Whole Cells
  7. Pre-vaccination and early B cell signatures predict antibody response to SARS-CoV-2 mRNA vaccine
    Authors: L Kardava, N Rachmanino, WW Lau, CM Buckner, K Trihemasav, FL de Assis, W Wang, X Zhang, Y Wang, CI Chiang, S Narpala, R Reger, GE McCormack, CA Seamon, RW Childs, AF Suffredini, JR Strich, DS Chertow, RT Davey, MC Sneller, S Oâ Connell, Y Li, A McDermott, TW Chun, AS Fauci, JS Tsang, S Moir
    medRxiv : the preprint server for health sciences, 2021-07-07;0(0):.
    Species: Human
    Sample Types: Whole Cells
  8. Modifying bacterial flagellin to evade Nod-like Receptor CARD 4 recognition enhances protective immunity against Salmonella
    Authors: P Tourlomous, JA Wright, AS Bittante, LJ Hopkins, SJ Webster, OJ Bryant, P Mastroeni, DJ Maskell, CE Bryant
    Nat Microbiol, 2020-10-26;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. Synthetic DNA Delivery of an Engineered Arginase Enzyme Can Modulate Specific Immunity In�Vivo
    Authors: M Khoshnejad, A Perales-Pu, Y Dia, P Xiao, A Patel, Z Xu, X Zhu, K Yun, I Baboo, R Qureshi, L Humeau, K Muthumani, DB Weiner
    Mol Ther Methods Clin Dev, 2020-06-01;18(0):652-663.
    Species: Mouse
    Sample Types: Whole Cells
  10. Enhancement of Immune Responses by Guanosine-Based Particles in DNA Plasmid Formulations against Infectious Diseases
    Authors: S Santos, M Ramírez, E Miranda, N Reyes, O Martínez, M Acosta-San, JM Rivera, M Otero
    J Immunol Res, 2019-05-22;2019(0):3409371.
    Species: Mouse
    Sample Types: Whole Cells
  11. Protective immunity by an engineered DNA vaccine for Mayaro virus
    Authors: H Choi, SB Kudchodkar, EL Reuschel, K Asija, P Borole, M Ho, K Wojtak, C Reed, S Ramos, NE Bopp, PV Aguilar, SC Weaver, JJ Kim, L Humeau, P Tebas, DB Weiner, K Muthumani
    PLoS Negl Trop Dis, 2019-02-07;13(2):e0007042.
    Species: Mouse
    Sample Types: Whole Cells
  12. Restoration of T Cell function in multi-drug resistant bacterial sepsis after interleukin-7, anti-PD-L1, and OX-40 administration
    Authors: LK Thampy, KE Remy, AH Walton, Z Hong, K Liu, R Liu, V Yi, CD Burnham, RS Hotchkiss
    PLoS ONE, 2018-06-26;13(6):e0199497.
    Species: Human
    Sample Types: Whole Cells
  13. Characterization of the immune response elicited by the vaccinia virus L3 protein delivered as naked DNA
    Authors: M Ramírez, S Santos, O Martínez, R Rodríguez, E Miranda, WD Ramos-Pere, M Otero
    Vaccine, 2018-03-07;36(15):2049-2055.
    Species: Mouse
    Sample Types: Whole Cells
  14. Rad52 deficiency decreases development of lung squamous cell carcinomas by enhancing immuno-surveillance
    Authors: R Lieberman, J Pan, Q Zhang, M You
    Oncotarget, 2017-05-23;8(21):34032-34044.
    Species: Mouse
    Sample Types: Whole Cells
  15. Donor bone marrow cells are essential for iNKT cell-mediated Foxp3+ Treg cell expansion in a murine model of transplantation tolerance
    Authors: S Miyairi, T Hirai, R Ishii, M Okumi, S Nunoda, K Yamazaki, Y Ishii, K Tanabe
    Eur. J. Immunol, 2017-03-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  16. Cerium Oxide Nanoparticles in Lung Acutely Induce Oxidative Stress, Inflammation, and DNA Damage in Various Organs of Mice
    Authors: A Nemmar, P Yuvaraju, S Beegam, MA Fahim, BH Ali
    Oxid Med Cell Longev, 2017-03-14;2017(0):9639035.
    Species: Mouse
    Sample Types: Tissue Homogenates
  17. Preclinical evaluation of multi antigenic HCV DNA vaccine for the prevention of Hepatitis C virus infection
    Authors: H Lee, M Jeong, J Oh, Y Cho, X Shen, J Stone, J Yan, Z Rothkopf, AS Khan, BM Cho, YK Park, DB Weiner, WC Son, JN Maslow
    Sci Rep, 2017-03-07;7(0):43531.
    Species: Mouse
    Sample Types: Whole Cells
  18. Effective Depletion of Pre-existing Anti-AAV Antibodies Requires Broad Immune Targeting
    Authors: VM Velazquez, AS Meadows, RJ Pineda, M Camboni, DM McCarty, H Fu
    Mol Ther Methods Clin Dev, 2017-01-25;4(0):159-168.
    Species: Mouse
    Sample Types: Whole Cells
  19. Characterization of guinea pig T cell responses elicited after EP-assisted delivery of DNA vaccines to the skin
    Authors: Trevor R F Smith
    Vaccine, 2016-11-25;35(1):61-70.
  20. CD4(+) and CD8(+) TCR? repertoires possess different potentials to generate extraordinarily high-avidity T cells
    Authors: M Nakatsugaw, MA Rahman, Y Yamashita, T Ochi, P Wnuk, S Tanaka, K Chamoto, Y Kagoya, K Saso, T Guo, M Anczurowsk, MO Butler, N Hirano
    Sci Rep, 2016-03-31;6(0):23821.
    Species: Human
    Sample Types: Whole Cells
  21. Autoreactive CD19+CD20- Plasma Cells Contribute to Disease Severity of Experimental Autoimmune Encephalomyelitis.
    Authors: Chen D, Ireland S, Davis L, Kong X, Stowe A, Wang Y, White W, Herbst R, Monson N
    J Immunol, 2016-01-13;196(4):1541-9.
    Species: Mouse
    Sample Types: Whole Cells
  22. HIV-1 Env DNA vaccine plus protein boost delivered by EP expands B- and T-cell responses and neutralizing phenotype in vivo.
    Authors: Muthumani, Kar, Wise, Megan C, Broderick, Kate E, Hutnick, Natalie, Goodman, Jonathan, Flingai, Seleeke, Yan, Jian, Bian, Chaoran, Mendoza, Janess, Tingey, Colleen, Wilson, Christin, Wojtak, Krzyszto, Sardesai, Niranjan, Weiner, David B
    PLoS ONE, 2013-12-31;8(12):e84234.
    Species: Mouse
    Sample Types: Whole Cells
  23. Proteome-wide screening reveals immunodominance in the CD8 T cell response against classical swine fever virus with antigen-specificity dependent on MHC class I haplotype expression.
    Authors: Franzoni G, Kurkure N, Essler S, Pedrera M, Everett H, Bodman-Smith K, Crooke H, Graham S
    PLoS ONE, 2013-12-23;8(12):e84246.
    Species: Porcine
    Sample Types: Whole Cells
  24. Nanoparticle vaccines encompassing the respiratory syncytial virus (RSV) G protein CX3C chemokine motif induce robust immunity protecting from challenge and disease.
    Authors: Jorquera P, Choi Y, Oakley K, Powell T, Boyd J, Palath N, Haynes L, Anderson L, Tripp R
    PLoS ONE, 2013-09-10;8(9):e74905.
    Species: Mouse
    Sample Types: Whole Cells
  25. Systemic immunization with CCL27/CTACK modulates immune responses at mucosal sites in mice and macaques.
    Authors: Kraynyak KA, Kutzler MA, Cisper NJ, Khan AS, Draghia-Akli R, Sardesal NY, Lewis MG, Yan J, Weiner DB
    Vaccine, 2010-02-23;28(8):1942-51.
  26. Immune responses to AAV in canine muscle monitored by cellular assays and noninvasive imaging.
    Authors: Wang Z, Storb R, Lee D, Kushmerick MJ, Chu B, Berger C, Arnett A, Allen J, Chamberlain JS, Riddell SR, Tapscott SJ
    Mol. Ther., 2009-12-29;18(3):617-24.
    Species: Canine
    Sample Types: Whole Cells
  27. Plasmid-encoded interleukin-15 receptor alpha enhances specific immune responses induced by a DNA vaccine in vivo.
    Authors: Kraynyak KA, Kutzler MA, Cisper NJ, Laddy DJ, Morrow MP, Waldmann TA, Weiner DB
    Hum. Gene Ther., 2009-10-01;20(10):1143-56.
    Species: Mouse
    Sample Types:
  28. Antiglioma immunological memory in response to conditional cytotoxic/immune-stimulatory gene therapy: humoral and cellular immunity lead to tumor regression.
    Authors: Ghulam Muhammad AK, Candolfi M, King GD, Yagiz K, Foulad D, Mineharu Y, Kroeger KM, Treuer KA, Nichols WS, Sanderson NS, Yang J, Khayznikov M, Van Rooijen N, Lowenstein PR, Castro MG
    Clin. Cancer Res., 2009-09-29;15(19):6113-27.
    Species: Rat
    Sample Types: Whole Cells
  29. Impact of the underlying mutation and the route of vector administration on immune responses to factor IX in gene therapy for hemophilia B.
    Authors: Cao O, Hoffman BE, Moghimi B, Nayak S, Cooper M, Zhou S, Ertl HC, High KA, Herzog RW
    Mol. Ther., 2009-07-14;17(10):1733-42.
    Species: Mouse
    Sample Types: Whole Cells
  30. Evidence for HIV-associated B cell exhaustion in a dysfunctional memory B cell compartment in HIV-infected viremic individuals.
    Authors: Moir S, Ho J, Malaspina A, Wang W, DiPoto AC, O'Shea MA, Roby G, Kottilil S, Arthos J, Proschan MA, Chun TW, Fauci AS
    J. Exp. Med., 2008-07-14;205(8):1797-805.
  31. Immunogenicity of novel consensus-based DNA vaccines against Chikungunya virus.
    Authors: Muthumani K, Lankaraman KM, Laddy DJ, Sundaram SG, Chung CW, Sako E, Wu L, Khan A, Sardesai N, Kim JJ, Vijayachari P, Weiner DB
    Vaccine, 2008-04-14;26(40):5128-34.
    Species: Mouse
    Sample Types: Whole Cells
  32. Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
    Authors: Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
    J. Immunol., 2008-04-01;180(7):5109-17.
    Species: Human
    Sample Types: Whole Cells
  33. DR5 activation of caspase-8 induces DC maturation and immune enhancement in vivo.
    Authors: Chattergoon MA, Muthumani K, Tamura Y, Ramanathan M, Shames JP, Saulino V, Robinson TM, Montaner LJ, Weiner DB
    Mol. Ther., 2007-12-18;16(2):419-26.
    Species: Mouse
    Sample Types: Whole Cells
  34. Vaccination with recombinant fusion proteins incorporating Toll-like receptor ligands induces rapid cellular and humoral immunity.
    Authors: Huleatt JW, Jacobs AR, Tang J, Desai P, Kopp EB, Huang Y, Song L, Nakaar V, Powell TJ
    Vaccine, 2006-08-22;25(4):763-75.
    Species: Mouse
    Sample Types: Whole Cells
  35. Cutting edge: macrophage migration inhibitory factor is necessary for progression of experimental autoimmune encephalomyelitis.
    Authors: Powell ND, Papenfuss TL, McClain MA, Gienapp IE, Shawler TM, Satoskar AR, Whitacre CC
    J. Immunol., 2005-11-01;175(9):5611-4.
  36. Mapping of immune responses following wild-type and mutant ABeta42 plasmid or peptide vaccination in different mouse haplotypes and HLA Class II transgenic mice.
    Authors: Kutzler MA, Cao C, Bai Y, Dong H, Choe PY, Saulino V, McLaughlin L, Whelan A, Choo AY, Weiner DB, Ugen KE
    Vaccine, 2005-08-24;24(21):4630-9.
    Species: Mouse
    Sample Types: Whole Cells
  37. Developing an epitope-driven tuberculosis (TB) vaccine.
    Authors: De Groot AS, McMurry J, Marcon L, Franco J, Rivera D, Kutzler M, Weiner D, Martin B
    Vaccine, 2005-03-18;23(17):2121-31.
    Species: Mouse
    Sample Types:
  38. Therapeutic effectiveness of recombinant cancer vaccines is associated with a prevalent T-cell receptor alpha usage by melanoma-specific CD8+ T lymphocytes.
    Authors: De Palma R, Marigo I, Del Galdo F, De Santo C, Serafini P, Cingarlini S, Tuting T, Lenz J, Basso G, Milan G, Zanovello P, Bronte V
    Cancer Res., 2004-11-01;64(21):8068-76.
    Species: Mouse
    Sample Types:
  39. Co-immunization with plasmid IL-12 generates a strong T-cell memory response in mice.
    Authors: Chattergoon MA, Saulino V, Shames JP, Stein J, Montaner LJ, Weiner DB
    Vaccine, 2004-04-16;22(13):1744-50.
    Species: Mouse
    Sample Types: Whole Cells
  40. Amelioration of graft versus host disease by galectin-1.
    Authors: Baum LG, Blackall DP, Arias-Magallano S, Nanigian D, Uh SY, Browne JM, Hoffmann D, Emmanouilides CE, Territo MC, Baldwin GC
    Clin. Immunol., 2003-12-01;109(3):295-307.

FAQs

No product specific FAQs exist for this product, however you may

View all ELISpot and FluoroSpot Kit FAQs
Loading...

Reviews for ELISpot Blue Color Module (Strep-AP and BCIP-NBT), 5 Plate

There are currently no reviews for this product. Be the first to review ELISpot Blue Color Module (Strep-AP and BCIP-NBT), 5 Plate and earn rewards!

Have you used ELISpot Blue Color Module (Strep-AP and BCIP-NBT), 5 Plate?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review