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dTAG Janelia Fluor® 635

Catalog #: 8101 Datasheet
Catalog # Availability Size / Price Qty
8101/100U
dTAG Janelia Fluor® 635 | Fluorescent Probes
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Description: Fluorogenic srTAG probe for live cell imaging of FBKP12 (F36V/L) labeled proteins

Chemical Name: 4-((2-(2-(3-((R)-3-(3,4-Dimethoxyphenyl)-1-(((S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)acetamido)ethyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate

Purity: ≥95%

Product Details
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Biological Activity Scientific Data Technical Data Additional Information Product Datasheets Calculators

Biological Activity

Key Information: dTAG Janelia Fluor® 635 is a fluorogenic srTAG probe for live cell imaging of FKBP12F36V/L fusion proteins.

Application: Suitable for confocal microscopy and Super Resolution Microscopy (SRM) including STED. dTAG Janelia Fluor® 635 is cell permeable.

Properties and Photophysical Data: In FRAP (fluorescence recovery after photobleaching) experiment, the highest maximum recovery ratio (85%) was shown when U2OS cells expressing FKBPF36L were incubated with 10 μM dTAG Janelia Fluor® 635; excitation and emission maxima (λ) are 640 nm and 665 nm, respectively; quantum yield = 0.41; extinction coefficient = 75,000 M-1cm-1 (in 4% TFA).

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

Scientific Data

Fluorescence Imaging Fluorescent microscopy data of dTAG Janelia Fluor® 635 in HEK293 cells View Larger

Application of dTAG Janelia Fluor® 635. HEK293 cells were incubated for 1 hour with 2.5 μM dTAG Janelia Fluor® 635 (red) and Hoechst (blue), without (left) or with (right) endogenous protein of interest labeled with dTAG/FKBP12F36V. Fluorescent microscopy images kindly provided by Yongli Shan, Vincinitas Therapeutics.

Fluorescence Imaging High content imaging data of dTAG Janelia Fluor® 635 in HEK293 cells. View Larger

Application of dTAG Janelia Fluor® 635. HEK293 cells were incubated for 1 hour with different concentrations of dTAG Janelia Fluor® 635 (red) and Hoechst (blue), without (top) or with (bottom) endogenous protein of interest labeled with dTAG/FKBP12F36V. Images captured by high content imaging were kindly provided by Yongli Shan, Vincinitas Therapeutics.

Optical Data for dTAG Janelia Fluor® 635

Emission Color Red
Brightness
λabs 640 nm
λem 665 nm
Extinction Coefficient (ε) 75000 M-1cm-1
Quantum Yield (φ) 0.41
Closest Laser line 635 nm
Cell Permeable Yes
Application Confocal Microscopy, Super Resolution Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
1250.18
Formula:
C69H77F2N5O13Si
Solubility:
Soluble to 10 mM in DMSO
Purity:
≥95%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

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