Home / DNA, RNA and Protein Synthesis Inhibitors / Cycloheximide

Cycloheximide

Catalog #: 0970
18 Citations Datasheet
Catalog # Availability Size / Price Qty
0970/100
Cycloheximide | CAS No. 66-81-9 | DNA, RNA and Protein Synthesis Inhibitors
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Description: Inhibitor of protein synthesis

Chemical Name: 4-[2-(3,5-Dimethyl-2-oxo-cyclohexyl)-2-hydroxyethyl]-2,6-piperidinedione

Purity: ≥97%

Product Details
Citations (18)
Reviews
Biological Activity Technical Data Background Product Datasheets Calculators

Biological Activity

Cycloheximide is a selective inhibitor of eukaryotic (over prokaryotic) protein synthesis, blocking tRNA binding and release from ribosomes. Induces apoptosis in a variety of transformed and normal cell lines, including T cells. Competitively inhibits the PPIase hFKBP12 (Ki = 3.4 μM). Also inhibits ferroptosis. Antifungal antibiotic. Exhibits anti-MERS-CoV activity in Vero cells in vitro (IC50 = 0.16 μM).

Technical Data

M.Wt:
281.35
Formula:
C15H23NO4
Solubility:
Soluble to 50 mM in ethanol and to 25 mM in water
Purity:
≥97%
Storage:
Desiccate at RT
CAS No:
66-81-9

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization
    S Yang, L Liu, C Cao, N Song, Y Wang, S Ma, Q Zhang, N Yu, X Ding, F Yang, S Tian, K Zhang, T Sun, J Yang, Z Yao, S Wu, L Shi
    Nat Commun, 2018;9(1):1285.
  2. Identification of Ras-degrading small molecules that inhibit the transformation of colorectal cancer cells independent of ?-catenin signaling
    W Shin, SK Lee, JH Hwang, JC Park, YH Cho, EJ Ro, Y Song, HR Seo, KY Choi
    Exp. Mol. Med., 2018;50(6):71.
  3. Synthesis and cytotoxic evaluation of cycloheximide derivatives as potential inhibitors of FKBP12 with neuroregenerative properties.
    Christner et al.
    J.Med.Chem., 1999;42:3615
  4. The mechanism by which cycloheximide and related glutarimide antibiotics inhibit peptide synthesis on reticulocyte ribosomes.
    Obrig et al.
    J.Biol.Chem., 1971;246:174
  5. Cycloheximide-induced T-cell death is mediated by a Fas-associated death domain-dependent mechanism.
    Tang et al.
    J.Biol.Chem., 1999;274:7245
  6. Ferroptosis: process and function.
    Xie et al.
    Cell.Death.Differ, 2016;23:369
  7. Coronavirus minus-strand RNA synthesis and effect of cycloheximide on coronavirus RNA synthesis.
    Sawicki and Sawicki et al.
    J.Virol., 1986;57:328

Product Datasheets

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SDS
⚠ WARNING: This product can expose you to chemicals including Cycloheximide, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Citations for Cycloheximide

The citations listed below are publications that use Tocris products. Selected citations for Cycloheximide include:

18 Citations: Showing 1 - 10

  1. AMPK activation regulates P-body dynamics in mouse sensory neurons in vitro and in vivo.
    Authors: Paige Et al.
    Neurobiol Pain  2019;5
  2. Staufen1 links RNA stress granules and autophagy in a model of neurodegeneration.
    Authors: Paul Et al.
    Nat Commun  2018;9:3648
  3. Rhomboid domain-containing protein 1 promotes breast cancer progression by regulating the p-Akt and CDK2 levels.
    Authors: Zhang Et al.
    Cell Commun Signal  2018;16:65
  4. Nongenomic Glucocorticoid Suppression of a Postsynaptic Potassium Current via Emergent Autocrine Endocannabinoid Signaling in Hypothalamic Neuroendocrine Cells following Chronic Dehydration.
    Authors: Wu and Tasker
    Eneuro  2017;4
  5. Acute Stress Suppresses Synaptic Inhibition and Increases Anxiety via Endocannabinoid Release in the Basolateral Amygdala.
    Authors: Di Et al.
    J.Neurosci.  2016;36:8461
  6. A balance of positive and negative regulators determines the pace of the segmentation clock.
    Authors: Wiedermann Et al.
    Elife  2015;4
  7. Activity-dependent rapid local RhoA synthesis is required for hippocampal synaptic plasticity.
    Authors: Briz Et al.
    Neurobiol Dis  2015;35:2269
  8. A molecular brake controls the magnitude of long-term potentiation.
    Authors: Wang Et al.
    Nat Commun  2014;5:3051
  9. Phosphorylation and degradation of tomosyn-2 de-represses Ins secretion.
    Authors: Bhatnagar Et al.
    J Biol Chem  2014;289:25276
  10. Opioid receptor function is regulated by post-endocytic peptide processing.
    Authors: Gupta Et al.
    J Biol Chem  2014;289:19613
  11. Long lasting protein synthesis- and activity-dependent spine shrinkage and elimination after synaptic depression.
    Authors: Ramiro-Cortés
    PLoS One  2013;8:e71155
  12. Phosphorylation of FMRP and alterations of FMRP complex underlie enhanced mLTD in adult rats triggered by early life seizures.
    Authors: Bernard Et al.
    J Appl Physiol (1985)  2013;59:42736
  13. Oxytocin-stimulated NFAT transcriptional activation in human myometrial cells.
    Authors: Pont Et al.
    Mol.Endocrinol.  2012;26:1743
  14. Dual regulation of fragile X mental retardation protein by group I metabotropic glutamate receptors controls translation-dependent epileptogenesis in the hippocampus.
    Authors: Zhao Et al.
    J Neurosci  2011;31:725
  15. Pharmacological and genetic accumulation of hypoxia-inducible factor-1alpha enhances excitatory synaptic transmission in hippocampal neurons through the production of vascular endothelial growth factor.
    Authors: Huang Et al.
    J Neurosci  2010;30:6080
  16. The effect of denervation on protein synthesis and degradation in adult rat diaphragm muscle.
    Authors: Argadine Et al.
    J Neurosci  2009;107:438
  17. Cyclooxygenase-2-derived prostaglandin F2alpha mediates endothelium-dependent contractions in the aortae of hamsters with increased impact during aging.
    Authors: Wong Et al.
    Circ Res  2009;104:228
  18. Heat shock protein 60 or 70 activates nitric-oxide synthase (NOS) I- and inhibits NOS II-associated signaling and depresses the mitochondrial apoptotic cascade during brain stem death.
    Authors: Chan Et al.
    J Neurosci  2007;282:4585

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