Cultrex Spheroid Formation Extracellular Matrix, 10X
Cultrex Spheroid Formation Extracellular Matrix, 10X Summary
Provides specialized extracellular matrix optimized to drive aggregation and/or spheroid formation of cells as a part of the Cultrex 3-D Culture Spheroid Cell Invasion Assay.Why Use Cultrex Spheroid Formation Extracellular Matrix, 10X?
The Cultrex 10X Spheroid Formation Extracellular Matrix (ECM) is a proprietary mixture of extracellular matrix proteins derived from murine EHS sarcoma and optimized for spheroid formation. The diluted ECM is a fluid in culture that promotes cell-cell adhesion within a low adhesion environment.
Negative for the presence of bacteria and fungi.
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Scientific Data
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Embedding Spheroids in Cultrex BME for Cell Invasion and Migration Assays. MDA-MB-231 (3,000 cells/well) formed spheroids in low adhesion plates for 72 hours using reagents in the Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay (Catalog # 3500-096-K). Briefly MDA-MB-231 cells were cultured in the presence of 1X Spheroid Formation Matrix (Catalog # 3500-096-01). Then spheroids were embedded in A) no Invasion Matrix, or B) Spheroid Invasion Matrix (Catalog # 3500-096-03). After hydrogel polymerization, DMEM and 10% FBS was added to each well to promote a chemotactic response over a 96 hour period. Note the addition of the Spheroid Invasion Matrix enhanced cell invasion over spheroids cultured without embedding in the invasion matrix.
Citations for Cultrex Spheroid Formation Extracellular Matrix, 10X
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Cell senescence-associated genes predict the malignant characteristics of glioblastoma
Authors: C Tan, Y Wei, X Ding, C Han, Z Sun, C Wang
Cancer Cell International, 2022-12-16;22(1):411. 2022-12-16
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PIM1-Induced Cytoplasmic Expression of RBMY Mediates Hepatocellular Carcinoma Metastasis
Authors: HH Chua, MH Chang, YH Chen, DJ Tsuei, YM Jeng, PH Lee, YH Ni
Cellular and Molecular Gastroenterology and Hepatology, 2022-10-01;0(0):. 2022-10-01
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DOCK6 promotes chemo- and radioresistance of gastric cancer by modulating WNT/beta-catenin signaling and cancer stem cell traits
Authors: HC Chi, CY Tsai, CS Wang, HY Yang, CH Lo, WJ Wang, KF Lee, LY Lai, JH Hong, YF Chang, MM Tsai, CT Yeh, CH Wu, CC Hsieh, LH Wang, WJ Chen, KH Lin
Oncogene, 2020-08-04;0(0):. 2020-08-04
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Co-delivery of IKBKE siRNA and cabazitaxel by hybrid nanocomplex inhibits invasiveness and growth of triple-negative breast cancer
Authors: Z Zhao, Y Li, H Liu, A Jain, PV Patel, K Cheng
Sci Adv, 2020-07-15;6(29):eabb0616. 2020-07-15
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Hypoxia Associated Factor (HAF) mediates neurofibromin ubiquitination and degradation leading to Ras-ERK pathway activation in hypoxia
Authors: Y Song Green, T Sargis, EC Reichert, E Rudasi, D Fuja, E Jonasch, MY Koh
Mol. Cancer Res., 2019-01-31;0(0):. 2019-01-31
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