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Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well

Catalog #: 3500-096-K
53 Citations 2 Reviews Datasheet
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3500-096-K
Spheroid Cell Invasion Assay_3500-096-K
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Citations (53)
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Reviews (2)
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Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well Summary

Provides a more complete and physiologically predictive cancer invasion model.

Why Use Cultrex 3-D Spheroid Basememnt Membrane Extract Cell Invasion Assay, 96-well?

The Cultrex 3-D Culture Spheroid Cell Invasion Assay utilizes a 3-D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation Extracellular Matrix to drive aggregation and/or spheroid formation of cells. Upon completion of spheroid formation, the spheroid is embedded in an invasion matrix composed of basement membrane proteins. This matrix forms a hydrogel network on which invasive cells can travel. Invasion modulating agents can be applied to the system to evaluate the impact on cell response. Cell invasion is visualized microscopically and can be quantified through image analysis software.

Kit Contents

• Cultrex Spheroid Formation Matrix
• 3-D Culture Qualified 96 Well Spheroid Formation Plate with Strip Seals
• Cultrex Spheroid Invasion Matrix

Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Mouse

Limitations

For research use only. Not for diagnostic use.

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Scientific Data

N/A Spheroid Cell Invasion Assay_3500-096-K View Larger

Embedding Spheroids in Cultrex BME for Cell Invasion and Migration Assays. MDA-MB-231 (3,000 cells/well) formed spheroids in low adhesion plates for 72 hours using reagents in the Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay. Briefly MDA-MB-231 cells were cultured in the presence of 1X Spheroid Formation Matrix. Then spheroids were embedded in A) no Invasion Matrix, or B) Spheroid Invasion Matrix. After hydrogel polymerization, DMEM and 10% FBS was added to each well to promote a chemotactic response over a 96 hour period. Note the addition of the Spheroid Invasion Matrix enhanced cell invasion over spheroids cultured without embedding in the invasion matrix.

Citations for Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

53 Citations: Showing 1 - 10
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  1. Identifying PLAUR as a Pivotal Gene of Tumor Microenvironment and Regulating Mesenchymal Phenotype of Glioblastoma
    Authors: Fu, Z;Chen, Z;Ye, J;Ji, J;Ni, W;Lin, W;Lin, H;Lu, L;Zhu, G;Xie, Q;Yan, F;Chen, G;Liu, F;
    Cancers  2024-02-19
  2. Synergistic effects of combined cisplatin and Clinacanthus nutans extract on triple negative breast cancer cells
    Authors: Abu Bakar, NFAB;Yeo, ZL;Hussin, F;Madhavan, P;Lim, V;Jemon, K;Prabhakaran, P;
    Journal of Taibah University Medical Sciences  2023-12-01
  3. Inhibition of GCN2 Reveals Synergy with Cell-Cycle Regulation and Proteostasis
    Authors: Gauthier-Coles, G;Rahimi, F;Bröer, A;Bröer, S;
    Metabolites  2023-10-09
  4. Production of novel recombinant anti-EpCAM antibody as targeted therapy for breast cancer
    Authors: Mirzaei, R;Shafiee, S;Vafaei, R;Salehi, M;Jalili, N;Nazerian, Z;Muhammadnajad, A;Yadegari, F;Reza Esmailinejad, M;Farahmand, L;
    International immunopharmacology  2023-07-18
  5. Single-cell RNA sequencing identifies critical transcription factors of tumor cell invasion induced by hypoxia microenvironment in glioblastoma
    Authors: Zhang, Y;Zhang, B;Lv, C;Zhang, N;Xing, K;Wang, Z;Lv, R;Yu, M;Xu, C;Wang, Y;
    Theranostics  2023-06-26
  6. Glioblastoma remodelling of human neural circuits decreases survival
    Authors: Krishna, S;Choudhury, A;Keough, MB;Seo, K;Ni, L;Kakaizada, S;Lee, A;Aabedi, A;Popova, G;Lipkin, B;Cao, C;Nava Gonzales, C;Sudharshan, R;Egladyous, A;Almeida, N;Zhang, Y;Molinaro, AM;Venkatesh, HS;Daniel, AGS;Shamardani, K;Hyer, J;Chang, EF;Findlay, A;Phillips, JJ;Nagarajan, S;Raleigh, DR;Brang, D;Monje, M;Hervey-Jumper, SL;
    Nature  2023-05-03
  7. Valtrate, an iridoid compound in Valeriana, elicits anti-glioblastoma activity through inhibition of the PDGFRA/MEK/ERK signaling pathway
    Authors: X Liu, Y Hu, Z Xue, X Zhang, X Liu, G Liu, M Wen, A Chen, B Huang, X Li, N Yang, J Wang
    Journal of Translational Medicine, 2023-02-24;21(1):147.  2023-02-24
  8. Myeloma Microenvironmental TIMP1 Induces the Invasive Phenotype in Fibroblasts to Modulate Disease Progression
    Authors: R Ishihara, T Oda, Y Murakami, I Matsumura, S Watanabe, Y Asao, Y Masuda, N Gotoh, T Kasamatsu, H Takei, N Kobayashi, N Sasaki, T Saitoh, H Murakami, H Handa
    International Journal of Molecular Sciences, 2023-01-22;24(3):.  2023-01-22
  9. Targeting tumor-stroma communication by blocking endothelin-1 receptors sensitizes high-grade serous ovarian cancer to PARP inhibition
    Authors: P Tocci, C Roman, R Sestito, V Di Castro, A Sacconi, I Molineris, F Paolini, M Carosi, G Tonon, G Blandino, A Bagnato
    Cell Death & Disease, 2023-01-05;14(1):5.  2023-01-05
  10. TGFbeta Governs the Pleiotropic Activity of NDRG1 in Triple-Negative Breast Cancer Progression
    Authors: A López-Teja, C Griñán-Lis, A González-G, FE Cara, RJ Luque, C Rosa-Garri, JL Blaya-Cáno, A Navarro-Oc, M Valenzuela, M Parra-Lópe, J Calahorra, I Blancas, JA Marchal, S Granados-P
    International journal of biological sciences, 2023-01-01;19(1):204-224.  2023-01-01
  11. Molecular and metabolic alterations of 2,3-dihydroquinazolin-4(1H)-one derivatives in prostate cancer cell lines
    Authors: LA Dahabiyeh, W Hourani, W Darwish, F Hudaib, B Abu-Irmail, PK Deb, KN Venugopala, V Mohanlall, R Abu-Dahab, MH Semreen, Y Bustanji
    Scientific Reports, 2022-12-14;12(1):21599.  2022-12-14
  12. PIM1-Induced Cytoplasmic Expression of RBMY Mediates Hepatocellular Carcinoma Metastasis
    Authors: HH Chua, MH Chang, YH Chen, DJ Tsuei, YM Jeng, PH Lee, YH Ni
    Cellular and Molecular Gastroenterology and Hepatology, 2022-10-01;0(0):.  2022-10-01
  13. Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis
    Authors: S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek
    Journal of ovarian research, 2022-07-26;15(1):87.  2022-07-26
  14. Exosomal CXCL14 Contributes to M2 Macrophage Polarization through NF-kappaB Signaling in Prostate Cancer
    Authors: HY Tian, Q Liang, Z Shi, H Zhao
    Oncogene, 2022-05-27;2022(0):7616696.  2022-05-27
  15. STAT3 Enhances Sensitivity of Glioblastoma to Drug-Induced Autophagy-Dependent Cell Death
    Authors: J Remy, B Linder, U Weirauch, BW Day, BW Stringer, C Herold-Men, A Aigner, K Krohn, D Kögel
    Cancers, 2022-01-11;14(2):.  2022-01-11
  16. Ganoderma lucidum polysaccharides inhibit in vitro tumorigenesis, cancer stem cell properties and epithelial-mesenchymal transition in oral squamous cell carcinoma
    Authors: MR de Camargo, TF Frazon, KK Inacio, FR Smiderle, NG Amôr, TJ Dionísio, CF Santos, CO Rodini, VS Lara
    Journal of ethnopharmacology, 2021-12-12;286(0):114891.  2021-12-12
  17. RHPN1?AS1 promotes ovarian carcinogenesis by sponging miR?6884?5p thus releasing TOP2A mRNA
    Authors: S Cui, F Li
    Oncology reports, 2021-08-20;46(4):.  2021-08-20
  18. A phosphatidic acid-binding lncRNA SNHG9 facilitates LATS1 liquid-liquid phase separation to promote oncogenic YAP signaling
    Authors: RH Li, T Tian, QW Ge, XY He, CY Shi, JH Li, Z Zhang, FZ Liu, LJ Sang, ZZ Yang, YZ Liu, Y Xiong, Q Yan, X Li, HQ Ju, J Liu, LJ Wang, JZ Shao, W Wang, T Zhou, A Lin
    Cell Research, 2021-07-15;0(0):.  2021-07-15
  19. Identification of novel mutations and functional impacts of EPAS1 in colorectal cancer
    Authors: F Islam, V Gopalan, CT Lu, S Pillai, AK Lam
    Cancer Medicine, 2021-07-11;0(0):.  2021-07-11
  20. PD-L1 recruits phospholipase C and enhances tumorigenicity of lung tumors harboring mutant forms of EGFR
    Authors: S Ghosh, NB Nataraj, A Noronha, S Patkar, A Sekar, S Mukherjee, S Winograd-K, L Kramarski, A Verma, M Lindzen, DD Garcia, J Green, G Eisenberg, H Gil-Henn, A Basu, Y Lender, S Weiss, M Oren, M Lotem, B Geiger, E Ruppin, Y Yarden
    Cell Reports, 2021-05-25;35(8):109181.  2021-05-25
  21. TrkB/C-induced HOXC6 activation enhances the ADAM8-mediated metastasis of chemoresistant colon cancer cells
    Authors: GB Park, S Choi, YS Yoon, D Kim
    Molecular Medicine Reports, 2021-04-13;23(6):.  2021-04-13
  22. Endothelin-1 drives invadopodia and interaction with mesothelial cells through ILK
    Authors: I Masi, V Caprara, F Spadaro, L Chellini, R Sestito, A Zancla, A Rainer, A Bagnato, L Rosanò
    Cell Reports, 2021-03-02;34(9):108800.  2021-03-02
  23. DOCK6 promotes chemo- and radioresistance of gastric cancer by modulating WNT/beta-catenin signaling and cancer stem cell traits
    Authors: HC Chi, CY Tsai, CS Wang, HY Yang, CH Lo, WJ Wang, KF Lee, LY Lai, JH Hong, YF Chang, MM Tsai, CT Yeh, CH Wu, CC Hsieh, LH Wang, WJ Chen, KH Lin
    Oncogene, 2020-08-04;0(0):.  2020-08-04
  24. Co-delivery of IKBKE siRNA and cabazitaxel by hybrid nanocomplex inhibits invasiveness and growth of triple-negative breast cancer
    Authors: Z Zhao, Y Li, H Liu, A Jain, PV Patel, K Cheng
    Sci Adv, 2020-07-15;6(29):eabb0616.  2020-07-15
  25. Integrated pharmaco-proteogenomics defines two subgroups in isocitrate dehydrogenase wild-type glioblastoma with prognostic and therapeutic opportunities
    Authors: S Oh, J Yeom, HJ Cho, JH Kim, SJ Yoon, H Kim, JK Sa, S Ju, H Lee, MJ Oh, W Lee, Y Kwon, H Li, S Choi, JH Han, JH Chang, E Choi, J Kim, NG Her, SH Kim, SG Kang, E Paek, DH Nam, C Lee, HS Kim
    Nat Commun, 2020-07-03;11(1):3288.  2020-07-03
  26. Targeting platelets for improved outcome in KRAS-driven lung adenocarcinoma
    Authors: SR Hyslop, M Alexander, AA Thai, A Kersbergen, AJ Kueh, MJ Herold, J Corbin, P Gangatirka, AP Ng, BJ Solomon, WS Alexander, KD Sutherland, EC Josefsson
    Oncogene, 2020-06-13;0(0):.  2020-06-13
  27. Cullin-7 (CUL7) is overexpressed in glioma cells and promotes tumorigenesis via NF-&kappaB activation
    Authors: J Xu, Z Zhang, M Qian, S Wang, W Qiu, Z Chen, Z Sun, Y Xiong, C Wang, X Sun, R Zhao, H Xue, G Li
    J. Exp. Clin. Cancer Res., 2020-04-06;39(1):59.  2020-04-06
  28. PAICS, a Purine Nucleotide Metabolic Enzyme, is Involved in Tumor Growth and the Metastasis of Colorectal Cancer
    Authors: S Agarwal, BVSK Chakravart, M Behring, HG Kim, DS Chandrashe, N Gupta, P Bajpai, A Elkholy, SAH Balasubram, C Hardy, SA Diffalha, S Varambally, U Manne
    Cancers (Basel), 2020-03-25;12(4):.  2020-03-25
  29. Targeting P4HA1 with a Small Molecule Inhibitor in a Colorectal Cancer PDX Model
    Authors: S Agarwal, M Behring, HG Kim, P Bajpai, BVSK Chakravart, N Gupta, A Elkholy, S Al Diffalh, S Varambally, U Manne
    Transl Oncol, 2020-03-18;13(4):100754.  2020-03-18
  30. Hypoxia-induced lncRNA PDIA3P1 promotes mesenchymal transition via sponging of miR-124-3p in glioma
    Authors: S Wang, Y Qi, X Gao, W Qiu, Q Liu, X Guo, M Qian, Z Chen, Z Zhang, H Wang, J Xu, H Xue, X Guo, P Zhang, R Zhao, G Li
    Cell Death Dis, 2020-03-03;11(3):168.  2020-03-03
  31. Nitidine Chloride Is a Potential Alternative Therapy for Glioma Through Inducing Endoplasmic Reticulum Stress and Alleviating Epithelial-Mesenchymal Transition
    Authors: Z Chen, J Zhang, H Xue, M Qian, X Guo, X Gao, J Xu, Y Qi, X Sun, G Li
    Integr Cancer Ther, 2020-01-01;19(0):1534735419900.  2020-01-01
  32. Aspartate &beta-hydroxylase promotes pancreatic ductal adenocarcinoma metastasis through activation of SRC signaling pathway
    Authors: K Ogawa, Q Lin, L Li, X Bai, X Chen, H Chen, R Kong, Y Wang, H Zhu, F He, Q Xu, L Liu, M Li, S Zhang, K Nagaoka, R Carlson, H Safran, K Charpentie, B Sun, J Wands, X Dong
    J Hematol Oncol, 2019-12-30;12(1):144.  2019-12-30
  33. ASPH-notch Axis guided Exosomal delivery of Prometastatic Secretome renders breast Cancer multi-organ metastasis
    Authors: Q Lin, X Chen, F Meng, K Ogawa, M Li, R Song, S Zhang, Z Zhang, X Kong, Q Xu, F He, X Bai, B Sun, MC Hung, L Liu, J Wands, X Dong
    Mol. Cancer, 2019-11-07;18(1):156.  2019-11-07
  34. Bacopasides I and II Act in Synergy to Inhibit the Growth, Migration and Invasion of Breast Cancer Cell Lines
    Authors: HM Palethorpe, E Smith, Y Tomita, M Nakhjavani, AJ Yool, TJ Price, JP Young, AR Townsend, JE Hardingham
    Molecules, 2019-09-30;24(19):.  2019-09-30
  35. MicroRNA-214 targets PTK6 to inhibit tumorigenic potential and increase drug sensitivity of prostate cancer cells
    Authors: P Cagle, S Niture, A Srivastava, M Ramalinga, R Aqeel, L Rios-Colon, U Chimeh, S Suy, SP Collins, R Dahiya, D Kumar
    Sci Rep, 2019-07-05;9(1):9776.  2019-07-05
  36. Identification of a Novel Fusion Gene, FAM174A-WWC1, in Early-Onset Colorectal Cancer: Establishment and Characterization of Four Human Cancer Cell Lines from Early-Onset Colorectal Cancers
    Authors: SC Kim, R Shin, HY Seo, M Kim, JW Park, SY Jeong, JL Ku
    Transl Oncol, 2019-06-20;12(9):1185-1195.  2019-06-20
  37. Low-dose zoledronate for the treatment of bone metastasis secondary to prostate cancer
    Authors: E Akoury, P Ahangar, A Nour, J Lapointe, KP Guérard, L Haglund, DH Rosenzweig, MH Weber
    Cancer Cell Int., 2019-02-08;19(0):28.  2019-02-08
  38. Isoliquiritin Apioside Suppresses in vitro Invasiveness and Angiogenesis of Cancer Cells and Endothelial Cells
    Authors: A Kim, JY Ma
    Front Pharmacol, 2018-12-10;9(0):1455.  2018-12-10
  39. Regulation of extracellular matrix degradation and metastatic spread by IQGAP1 through endothelin-1 receptor signaling in ovarian cancer.
    Authors: Chellini L, Caprara V, Spadaro F, Sestito R, Bagnato A, Rosano L
    Matrix Biol, 2018-10-25;0(0):.  2018-10-25
  40. Repositioning Bazedoxifene as a novel IL-6/GP130 signaling antagonist for human rhabdomyosarcoma therapy
    Authors: H Xiao, HK Bid, X Chen, X Wu, J Wei, Y Bian, C Zhao, H Li, C Li, J Lin
    PLoS ONE, 2017-07-03;12(7):e0180297.  2017-07-03
  41. The effect of bone morphogenetic protein-2 on osteosarcoma metastasis.
    Authors: Gill J, Connolly P, Roth M, Chung S, Zhang W, Piperdi S, Hoang B, Yang R, Guzik H, Morris J, Gorlick R, Geller D
    PLoS ONE, 2017-03-06;12(3):e0173322.  2017-03-06
  42. The LINK-A lncRNA interacts with PtdIns(3,4,5)P3 to hyperactivate AKT and confer resistance to AKT inhibitors.
    Authors: Lin A, Hu Q, Li C, Xing Z, Ma G, Wang C, Li J, Ye Y, Yao J, Liang K, Wang S, Park P, Marks J, Zhou Y, Zhou J, Hung M, Liang H, Hu Z, Shen H, Hawke D, Han L, Zhou Y, Lin C, Yang L
    Nat Cell Biol, 2017-02-20;19(3):238-251.  2017-02-20
  43. Estradiol promotes breast cancer cell migration via recruitment and activation of neutrophils
    Authors: G Vazquez Ro, A Abrahamsso, LD Jensen, C Dabrosin
    Cancer Immunol Res, 2017-02-03;0(0):.  2017-02-03
  44. The GAS6-AXL signaling network is a mesenchymal (Mes) molecular subtype-specific therapeutic target for ovarian cancer.
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  45. Musashi-2 (MSI2) supports TGF-beta signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis.
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    Authors: Hiroaki Kajiho
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    Endocr Relat Cancer, 2015-11-11;23(2):113-24.  2015-11-11
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FAQs

  1. For Cultrex 3D BME Cell Invasion Assay, 96 well,catalog # 3500-096-K,  do we measure the protein concentration of the invasion matrix (part # 3500-096-03) supplied in this kit?

    • No. We donot measure the protein concentration of the invasion matrix (part # 3500-096-03) supplied in catalog # 3500-096-K.

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